Tailoring the surface architecture of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) scaffolds

Abstract

This study was designed to determine whether the surface modifications of the various poly (3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer scaffolds fabricated would enhance mouse fibroblast cells (L929) attachment and proliferation. The P(3HB-co-4HB) copolymer with a wide range of 4HB monomer composition (16-91 mol %) was synthesized by a local isolate Cupriavidus sp. USMAA1020 by employing the modified two-stage cultivation and by varying the concentrations of 4HB precursors, namely c-butyrolactone and 1,4-butanediol. Five different processing techniques were used in fabricating the P(3HB-co-4HB) copolymer scaffolds such as solvent casting, salt-leaching, enzyme degradation, combining salt-leaching with enzyme degradation, and electrospinning. The increase in 4HB composition lowered melting temperatures (T m ) but increased elongation to break. P(3HB-co-91 mol % 4HB) exhibited a melting point of 46C and elongation to break of 380%. The atomic force analysis showed an increase in the average surface roughness as the 4HB monomer composition increased. The mouse fibroblasts (L929) cell attachment was found to increase with high 4HB monomer composition in copolymer scaffolds. These results illustrate the importance of a detailed characterization of surface architecture of scaffolds to provoke specific cellular responses.

Synthesis of P(3HB‐co‐4HB) copolymer with target‐specific 4HB molar fractions using combinations of carbon substrates

“…Poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate) [P(3HB‐ co ‐4HB)] is one of the PHAs that gained attention as a promising biomaterial especially in the medical and pharmaceutical application. P(3HB‐ co ‐4HB) has gained interest as cardiovascular patches, drug delivery and tissue engineering application . The incorporation of 4HB monomers into the P(3HB) improves the material application potentials as the copolymer showed a wide range of physical properties from crystalline to rubber‐like material .…”

“…P(3HB-co-4HB) has gained interest as cardiovascular patches, drug delivery and tissue engineering application. 5…”

Synthesis of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate)/chitosan/silver nanocomposite material with enhanced antimicrobial activity

“…Tensile strength, Young's modulus and elongation at break were deduced from the stress/strain curves. The average value of five replicates was taken for each sample …”

PHB (poly‐β‐hydroxybutyrate) and its enzymatic degradation

“…The perspective area of PHB application is in development of implanted medical devices as biodegradable screws and plates for cartilage and bone, biodegradable membranes for periodontal treatment, biodegradable surgical sutures for dental, orthopaedic, cranio‐maxillofacial, hernioplastic, and skin surgery (Figure ). The PHB offers great potential in modern pharmacology for creating novel systems for sustained delivery of antiproliferative and anti‐inflammatory drugs in form of PHB films . The release of drugs occurs by methods, diffusion, and degradation, operating simultaneously.…”

“…The PHB offers great potential in modern pharmacology for creating novel systems for sustained delivery of antiproliferative and anti-inflammatory drugs in form of PHB films. 58 The release of drugs occurs by methods, diffusion, and degradation, operating simultaneously. The rate of the release of a drug depends on its nature, the thickness of the PHB films containing the drug, the weight ratio drug and polymer, and the molecular weight of PHB.…”