Plasma‐treated, collagen‐anchored polylactone: Its cell affinity evaluation under shear or shear‐free conditions

Abstract

Poly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and "intuitionistic" dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH(3) plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods.

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

“…The surface of the NH 3 plasma pretreatment was reported to be easier to anchor collagen. 24,25 Culture of mouse 3T3 fibroblasts demonstrated that the method can effectively facilitate cell migration and that the cultured cells can be distributed evenly throughout the scaffold. These studies disclosed that plasma treatment was helpful in promotion of bioactive macromolecules to the surface of synthesized polymers.…”

Engineering cell attachments to scaffolds in cartilage tissue engineering

“…Typical surface modification methods include ethanol treatment, 84 alkaline hydrolysis, 85 and plasma oxidization. 86 In plasma oxidization of polystyrene, for example, the outermost surface of the polymer is disrupted and hydrophilic functional groups are produced which can interact strongly with proteins such as fibronectin 87,88 and collagen 89 to improve cell anchorage. Ultimately, a balance between hydrophobicity and hydrophilicity is required for cell attachment through protein adsorption; Groth et al 81 identified the optimal water contact angle as 55-75º based on a fibroblast study.…”

Chitosan-Modified PLGA Nanoparticles with Versatile Surface for Improved Drug Delivery

“…Gas plasma or plasma polymerization has been used to modify and functionalize PLGA surfaces (6)(7)(8). The retention of plasma generated functional groups on PLGA surface appears quite weak (9,10), thus making it hard to have targeting molecule conjugation.…”