Engineering cell attachments to scaffolds in cartilage tissue engineering

Steward, Andrew J.; Liu, Yongxing; Wagner, Diane R.

doi:10.1007/s11837-011-0062-x

Cited by 50 publications

(46 citation statements)

References 114 publications

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“…Cell-matrix interactions alone are known to play a key role in determining stem cell fate [13]. Fibrin better supported MSC proliferation and collagen production relative to those in agarose; however MSCs in agarose produced significantly more sGAG.…”

Section: Discussionmentioning

confidence: 98%

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Cell–matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

et al. 2012

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Section: Discussionmentioning

confidence: 98%

“…Cell-matrix interactions also play a key role in the chondrogenic differentiation of MSCs [13]. Arginine-glycine-aspartic acid (RGD) is an amino acid sequence that integrins are known to bind to, and it is commonly used to allow cells to adhere to scaffolds that do not have any binding sites.…”

Section: Introductionmentioning

confidence: 99%

Cell–matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

et al. 2012

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“…In the superficial zone, near the articular surface, the collagen fibers are mostly aligned parallel to the surface and contribute in resisting shear stresses. In the middle zone, the fibers are more randomly aligned to resist isotropic pressure from compressive loads, and in the deep zone the fibers aligned perpendicular to the surface plays an important role in securing the cartilage to the bone by anchoring the collagen fibrils to the subchondral bone [3,4]. Articular cartilage covers the end of the long bones and act as a smooth (low friction) bearing surface to facilitate the movement of the joint.…”

Section: Introductionmentioning

confidence: 99%

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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Abstract:In a synovial joint, the articular cartilage is directly affected during the progression of Osteoarthritis (OA). The characterization of early stage modification in extra-cellular matrix of cartilage is essential for detection as well as understanding the progression of disease. The objective of this study is to demonstrate the potential and capability of nonlinear optical microscopy for the morphological investigation of early stage osteoarthritic cartilage. ICRS Grade-I cartilage sections were obtained from the femoral condyle of the human knee. The surface of articular cartilage was imaged by second harmonic generation and two-photon excited fluorescence microscopy. Novel morphological features like microsplits and wrinkles were observed, which would otherwise not be visible in other clinical imaging modalities (e.g., CT, MRI, ultrasound and arthroscope. The presence of superficial layer with distinct collagen fibrils parallel to the articular surface in 4 specimens out of 14 specimens, indicates that different phases of OA within ICRS Grade-I can be detected by SHG microscopy. All together, the observed novel morphologies in early stage osteoarthritic cartilage indicates that SHG microscopy might be a significant tool for the assessment of cartilage disorder.

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“…those generated outside the cell and transmitted inwards via the pericellular matrix (PCM) and those generated within the cell in response to its substrate) have been well characterized as key regulators of mesenchymal stem/stromal cell (MSC) differentiation (Engler et al, 2006;Kelly and Jacobs, 2010;Reilly and Engler, 2010;Steward et al, 2011Steward et al, , 2012Steward et al, , 2013Thorpe et al, 2012). Dynamic compression (DC) has been shown to enhance chondrogenesis of MSCs depending on the timing and duration of its application (Campbell et al, 2006;Haugh et al, 2011;Huang et al, 2004Huang et al, , 2005Huang et al, , 2010Mouw et al, 2007;Thorpe et al, 2010); however, the underlying mechanisms responsible for this temporal mechanosensitivity are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Exploring the roles of integrin binding and cytoskeletal reorganization during mesenchymal stem cell mechanotransduction in soft and stiff hydrogels subjected to dynamic compression

Steward

Wagner

Kelly

2014

Journal of the Mechanical Behavior of Biomedical Materials

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Mesenchymal Mechanobiology Cytoskeleton Mechanotransduction Focal adhesion a b s t r a c tThe objective of this study was to explore how the response of mesenchymal stem cells (MSCs) to dynamic compression (DC) depends on their pericellular environment and the development of their cytoskeleton. MSCs were first seeded into 3% agarose hydrogels, stimulated with the chondrogenic growth factor TGF-β3 and exposed to DC ( $10% strain at 1 Hz) for 1 h on either day 7, 14, or 21 of culture. At each time point, the actin, vimentin and tubulin networks of the MSCs were assessed using confocal microscopy. Similar to previous results, MSCs displayed a temporal response to DC; however, no dramatic changes in gross cytoskeletal organization were observed with time in culture. Vinculin (a membrane-cytoskeletal protein in focal adhesions) staining appeared more intense with time in culture. We next aimed to explore how changes to the pericellular environment, independent of the duration of exposure to TGF-β3, would influence the response of MSCs to DC. To this end, MSCs were encapsulated into either 'soft' or 'stiff' agarose hydrogels that are known to differentially support pericellular matrix (PCM) development. The application of DC led to greater relative increases in the expression of chondrogenic marker genes in the stiffer hydrogels, where the MSCs were found to have a more well developed PCM. These increases in gene expression were not observed following the addition of RGDS, an integrin blocker, suggesting that integrin binding plays a role in determining the response of MSCs to DC. Microtubule organization in MSCs was found to adapt in response to DC, but this effect was not integrin mediated, as this cytoskeletal reorganization was also observed in the presence of RGDS. In conclusion, although the PCM, integrin binding, and cytoskeletal reorganization are all involved in mechanotransduction of DC, none of these factors in isolation was able to completely explain the temporal mechanosensitivity of MSCs to dynamic compression.& 2013 Elsevier Ltd. All rights reserved. j o u r n a l o f t h e m e c h a n i c a l b e h a v i o r o f b i o m e d i c a l m a t e r i a l sPlease cite this article as: Steward, A.J., et al., Exploring the roles of integrin binding and cytoskeletal reorganization during mesenchymal stem cell mechanotransduction in soft and stiff hydrogels subjected to dynamic compression.

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Engineering cell attachments to scaffolds in cartilage tissue engineering

Cited by 50 publications

References 114 publications

Cell–matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

Cell–matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Exploring the roles of integrin binding and cytoskeletal reorganization during mesenchymal stem cell mechanotransduction in soft and stiff hydrogels subjected to dynamic compression

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