Extremozymes and Extremoproteins in Biosensor Applications

Vasudevan, Namasivayam; Annamalai, Jayshree

doi:10.1002/9781119143802.ch72

Cited by 12 publications

(7 citation statements)

References 118 publications

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“…Genomic and structural analysis have recently established that enzymes of halophilic bacteria are negatively charged due to excessive acidic residues [ 23 ], thereby enhancing their solubility and allowing for the formation of a solvation shell to maintain the enzyme stability under high salinity conditions [ 85 ]. Such novel halophilic enzymes isolated from halotolerant microorganisms could provide enzyme stability and activity and the ability to operate as biosensing elements under extreme, harsh conditions, opening up new exciting opportunities for the design of enzymatic biosensors [ 86 ]. Finally, halophilic enzymes could also be expressed on cell surfaces via surface expression anchors, where microorganisms can serve as a support matrix for the enzymes.…”

Section: Future Directions and Perspectives On Utilizing Halophilimentioning

confidence: 99%

The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing

Gaffney

Simoska

2021

Biosensors

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Halophilic bacteria are remarkable organisms that have evolved strategies to survive in high saline concentrations. These bacteria offer many advances for microbial-based biotechnologies and are commonly used for industrial processes such as compatible solute synthesis, biofuel production, and other microbial processes that occur in high saline environments. Using halophilic bacteria in electrochemical systems offers enhanced stability and applications in extreme environments where common electroactive microorganisms would not survive. Incorporating halophilic bacteria into microbial fuel cells has become of particular interest for renewable energy generation and self-powered biosensing since many wastewaters can contain fluctuating and high saline concentrations. In this perspective, we highlight the evolutionary mechanisms of halophilic microorganisms, review their application in microbial electrochemical sensing, and offer future perspectives and directions in using halophilic electroactive microorganisms for high saline biosensing.

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Section: Future Directions and Perspectives On Utilizing Halophilimentioning

confidence: 99%

The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing

Gaffney

Simoska

2021

Biosensors

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“…The receiver operating characteristic (ROC) curve of comparative analyses of baseline models is shown in Figure 6. The entire computational experiments were conducted by Python programming language [93] (for training and testing the model).…”

Section: Results Of Random Forest Classification Of Symptomatic and N...mentioning

confidence: 99%

Biosensors: 10th Anniversary Feature Papers

2022

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“…Thermozymes possess remarkable properties such as high thermostability, substrate selectivity, and elevated enzymatic activity [ 13 – 15 ]. They are not prone to proteolysis and chemical denaturation in the presence of detergents, extreme pH, chaotropic agents, and organic solvents [ 16 – 18 ]. Thermozymes can retain native protein structure and function at extreme temperatures because the protein is highly stabilized.…”

Section: Bio-fabrication Of Thermozyme-based Nano-biosensors: Essential Componentsmentioning

confidence: 99%

Bio-fabrication of thermozyme-based nano-biosensors: their components and present scenario

Soy

Sharma

Nigam

2022

J Mater Sci: Mater Electron

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An amalgamation of microbiology, biocatalysis, recombinant molecular biology, and nanotechnology is crucial for groundbreaking innovation in developing nano-biomedicines and sensoristics. Enzyme-based nano-biosensor finds prospective applications in various sectors (environmental, pharmaceutical, food, biorefineries). These applications demand reliable catalytic efficiency and functionality of the enzyme under an extreme operational environment for a prolonged period. Over the last few years, bio-fabrication of nano-biosensors in conjunction with thermozymes from thermophilic microbes is being sought after as a viable design. Thermozymes are known for their robustness, are chemically resistant toward organic solvents, possess higher durability for constant use, catalytic ability, and stability at elevated temperatures. Additionally, several other attributes of thermozymes like substrate specificity, selectivity, and sensitivity make them desirable in developing a customized biosensor. In this review, crucial designing aspects of enzyme-based nano-biosensors like enzyme immobilization on an electrode surface, new materials derived from microbial sources (biopolymers based nanocomposites), improvisation measures for sensitivity, and selectivity have been addressed. It also covers microbial biosynthesis of nanomaterials used to develop sensoristic devices and its numerous applications such as wastewater treatment, biorefineries, and diagnostics. The knowledge will pave the way toward creating consistent eco-friendly, economically viable nanostructured-based technologies with broad applicability and exploitation for industrial use in the near future.

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Extremozymes and Extremoproteins in Biosensor Applications

Cited by 12 publications

References 118 publications

The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing

The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing

Biosensors: 10th Anniversary Feature Papers

Bio-fabrication of thermozyme-based nano-biosensors: their components and present scenario

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