The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing

Gaffney, Erin M.; Simoska, Olja

doi:10.3390/bios11020048

Cited by 16 publications

(11 citation statements)

References 91 publications

(155 reference statements)

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“…Current density production was observed at a negative potential (-0.16 V/SHE) after two or three days of polarization. This is in agreement with the results of Torres et al (2009) and Gaffney et al 2021, who observed a faster start-up at lower potentials when marine sediments were used as inoculum. Maximum current density peaks were reached after 16 days of polarization.…”

Section: Halotolerant Bioanode Growthsupporting

confidence: 93%

Microbial electrochemical system for obtaining value-added products using a halotolerant bioanode

González¹,

Escobar²,

Tapia-Tussell³

et al. 2021

RMIQ

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Section: Halotolerant Bioanode Growthsupporting

confidence: 93%

Microbial electrochemical system for obtaining value-added products using a halotolerant bioanode

González¹,

Escobar²,

Tapia-Tussell³

et al. 2021

RMIQ

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“…Biohybrid electrochemical systems, where biological catalysts are coupled to abiotic electrodes, represent a sustainable approach for a variety of technological applications spanning from biosensing and water quality monitoring, − bioelectrosynthesis, − and micro to low power generation. − Additionally, the use of photosynthetic entities as the biocatalyst allows utilizing sunlight, one of the most attractive energy sources, to power such systems, paving the way to the field of semiartificial photosynthesis. − Using whole, metabolically active, microorganisms greatly simplifies the preparation of the biocatalyst (no enzyme isolation/purification required) and potentially enhances stability of the system thanks to their self-repairing and replication features. Purple nonsulfur bacteria have been used as model organisms for studying bacterial photosynthesis. , Additionally, purple bacteria are of great interest for their potential application for H 2 synthesis, , as well as bioremediation and biosensing, with Rhodobacter capsulatus ( R. capsulatus ), representing a very interesting candidate as biophotocatalyst due to their extreme metabolic versatility .…”

Section: Introductionmentioning

confidence: 99%

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

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Interfacing intact and metabolically active photosynthetic bacteria with abiotic electrodes requires both establishing extracellular electron transfer and immobilizing the biocatalyst on electrode surfaces. Artificial approaches for photoinduced electron harvesting through redox polymers reported in literature require the separate synthesis of artificial polymeric matrices and their subsequent combination with bacterial cells, making the development of biophotoanodes complex and less sustainable. Herein, we report a one-pot biocompatible and sustainable approach, inspired by the byssus of mussels, that provides bacterial cells adhesion on multiple surfaces under wet conditions to obtain biohybrid photoanodes with facilitated photoinduced electron harvesting. Purple bacteria were utilized as a model organism, as they are of great interest for the development of photobioelectrochemical systems for H 2 and NH 3 synthesis, biosensing, and bioremediation purposes. The polydopamine matrix preparation strategy allowed the entrapment of active purple bacteria cells by initial oxygenic polymerization followed by electrochemical polymerization. Our results unveil that the deposition of bacterial cells with simultaneous polymerization of polydopamine on the electrode surface enables a 5-fold enhancement in extracellular electron transfer at the biotic/abiotic interface while maintaining the viability of the cells. The presented approach paves the way for a more sustainable development of biohybrid photoelectrodes.

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“…The understanding of microorganisms' metabolism has increased the reaction’s efficiency [ 7 , 8 ]. The use of MFCs for electricity production is still not optimized, and the amount of electric current generated by this system is low; however, the potential of such systems is great.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the optimum conditions for electricity generation by haloalkaliphilic archaeon Natrialba sp. GHMN55 using the Plackett–Burman design: single and stacked MFCs

2022

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The production of bioelectricity via the anaerobic oxidation of organic matter by microorganisms is recently receiving much interest and is considered one of the future alternative technologies. In this study, we aimed to produce electrical current by using facultative halophilic archaeon Natrialba sp. GHMN55 as a biocatalyst at the anode of a microbial fuel cell (MFC) to generate electrons from the anaerobic breakdown of organic matter to produce electrical current. Since the MFC’s performance can be affected by many factors, the Plackett–Burman experimental design was applied to optimize the interaction between these factors when tested together and to identify the most significant factors that influence bioelectricity generation. We found that the factors that significantly affected electrical current generation were casein, inoculum age, magnet-bounded electrodes, NaCl, resistor value, and inoculum size; however, the existence of a mediator and the pH showed negative effects on bioelectricity production, where the maximum value of the 200 mV voltage was achieved after 48 h. The optimum medium formulation obtained using this design led to a decrease in the time required to produce bioelectricity from 20 days (in the basal medium) to 2 days (in the optimized medium). Also, the overall behavior of the cell could be enhanced by using multiple stacked MFCs with different electrical configurations (such as series or parallel chambers) to obtain higher voltages or power densities than the single chambers where the series chambers were recorded at 27.5 mV after 48 h of incubation compared with 12.6 mV and 1.1 mV for parallel and single chambers, respectively. These results indicate that the order of preferred MFC designs regarding total power densities would be series > parallel > single.

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The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing

Cited by 16 publications

References 91 publications

Microbial electrochemical system for obtaining value-added products using a halotolerant bioanode

Microbial electrochemical system for obtaining value-added products using a halotolerant bioanode

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Investigation of the optimum conditions for electricity generation by haloalkaliphilic archaeon Natrialba sp. GHMN55 using the Plackett–Burman design: single and stacked MFCs

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