Extrapolation from Experimental Chronic Staphylococcal Mastitis in Mice to Experimental Infections in Cattle

Anderson, James C.; Heneghan, D.J.S.

doi:10.1016/s0007-1935(17)30005-2

Cited by 11 publications

(6 citation statements)

References 5 publications

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“…For these reasons, the pathogen can efficiently colonise the MG by staying, at least for a time, a step ahead of the innate immune response. Of interest, the bovine MG contains more resident phagocytic cells than the mouse, and chronic subclinical infections are more likely to occur [20]. The intracellular component of infection is believed to play a significant role in the persistence of S. aureus during antibiotic therapy for different types of infections, including bovine mastitis [21].…”

Section: Fundamental Observations Concerning the Mmsammentioning

confidence: 99%

The pathogenesis and control of Staphylococcus aureus-induced mastitis: study models in the mouse

Brouillette

Malouin

2005

Microbes and Infection

105

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Section: Fundamental Observations Concerning the Mmsammentioning

confidence: 99%

The pathogenesis and control of Staphylococcus aureus-induced mastitis: study models in the mouse

Brouillette

Malouin

2005

Microbes and Infection

105

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“…Although acute mastitis can sometimes be treated, chronic mastitis has a cure rate of only 50% (9) or less (21). It has been suggested (1,4,19) that chronicity may be due to survival of the microbe within phagocytes. This intracellular location may afford the microbe protection from the effects of the antibiotic (5,8).…”

mentioning

confidence: 99%

Evaluation of antibacterial agents in a high-volume bovine polymorphonuclear neutrophil Staphylococcus aureus intracellular killing assay

Sanchez¹,

Ford²,

Yancey³

1986

Antimicrob Agents Chemother

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A bovine polymorphonuclear leukocyte (PMN) monolayer system was used to determine the ability of different antibiotics to kill surviving intracellular Staphylococcus aureus. The following classes of antimicrobial agents were tested in this high-volume assay procedure: aminocyclitol, I(-lactam, coumarin, lincosaminide, macrolide, naphthalenic ansamycin, paulomycin, peptide, quinolone, and tetracycline. The activities of these compounds were compared with those of positive (rifampin), negative (cloxacillin), and non-antibiotic-treated controls. Only oxytetracycline, the ansamycins (rifampin, rifamycin SV, streptovaricin A, C, and D), paulomycin, and paldimycin caused a significant reduction in the viable count of intracellular S. aureus. Of these, however, the intracellular killing by the streptovaricins was directly related to the cytotoxicity (as determined by trypan blue exclusion) of these compounds for the PMNs. Although the paulomycins were cytotoxic for the PMNs, the cytotoxic and the intracellular killing activity of these new compounds could be distinguished. The relevance of these results to the therapeutic effectiveness of these antibiotics in the treatment of bovine staphylococcal mastitis is discussed.

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“…Incorporation within liposomes is a recognized method of' directing the delivery of drugs in iriiro (Gregoriadis, 1977;Kimelberg & Mayhew, 1978). There is evidence from studies in uitro using macrophage nionolayers that the addition of antibiotics in liposomes enhances the intr;rcellular killing of S. a w e w (Bonventre 8c Gregoriadis, 1978; T h e essential feature of chronic mastitis is survival of the organisms in neutrophils and maci-ophages in the mammary gland (Anderson & Heneghan, 1979;Nickerson & Heald, 1981). I t was therefore considered that the al., 1981).…”

Section: N T R O D U C?'i 0 Nmentioning

confidence: 99%

“…The technique of producing mastitis in mice by intramammary inoculation of organisms isolated from clinical cases of bovine mastitis provides a reliable model of the disease in cattle (Anderson & Heneghan, 1979). New forms of therapy of bovine mastitis can therefore be first tested in mice; a successful outcome would indicate progression to clinical trials in dairy cattle.…”

Section: 25tmentioning

confidence: 99%

The effect of incorporation of cloxacillin in liposomes on treatment of experimental staphylococcal mastitis in mice

Anderson

Kirby

1986

Vet Pharm & Therapeutics

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The effect of incorporation of cloxacillin in liposomes on the treatment of staphylococcal mastitis was assessed bacteriologically 18 h after treatment of experimental infections in mice caused by two strains of Staphylococcus aureus. Intramammary treatments were cloxacillin incorporated in liposomes, cloxacillin in combination with liposomes, empty liposomes, cloxacillin in saline and saline alone. In none of the experiments did entrapment of cloxacillin within liposomes enhance its antibacterial effects. Electron microscopic studies demonstrated the presence of liposomes in neutrophils which also contained staphylococci. The results support the hypothesis that intracellular staphylococci are metabolically dormant and therefore not susceptible to the action of inhibitors of cell wall synthesis such as cloxacillin.

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Extrapolation from Experimental Chronic Staphylococcal Mastitis in Mice to Experimental Infections in Cattle

Cited by 11 publications

References 5 publications

The pathogenesis and control of Staphylococcus aureus-induced mastitis: study models in the mouse

The pathogenesis and control of Staphylococcus aureus-induced mastitis: study models in the mouse

Evaluation of antibacterial agents in a high-volume bovine polymorphonuclear neutrophil Staphylococcus aureus intracellular killing assay

The effect of incorporation of cloxacillin in liposomes on treatment of experimental staphylococcal mastitis in mice

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