Extracts from Amazonian plants have inhibitory activity against tyrosinase: an in vitro evaluation

Macrini, Daclé Juliani; Suffredini, Ivana Barbosa; Varella, Antonio Drauzio; Younes, Riad Naim; Ohara, Mitsuko Taba

doi:10.1590/s1984-82502009000400015

Cited by 20 publications

(22 citation statements)

References 15 publications

(20 reference statements)

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“…[46], with modifications. This method relies on the inhibition of tyrosinase in the presence of its substrate tyrosine, interrupting the synthesis of melanin.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Resveratrol Analogs on Mushroom Tyrosinase Activity

et al. 2012

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Skin pigmentation disorders typically involve an overproduction or uneven distribution of melanin, which results in skin spots. Resveratrol can inhibit tyrosinase, the active enzyme in the synthesis of melanin, but it does not inhibit the synthesis of melanin to an extent that enables its use alone as a skin whitening agent in pharmaceutical formulations, so its use as a coadjuvant in treatment of hyperpigmentation is suggested. Six resveratrol analogs were tested for tyrosinase inhibitory activity in vitro. Among the analogs tested, compound D was the most powerful tyrosinase inhibitor (IC50 = 28.66 µg/mL), two times more active than resveratrol (IC50 = 57.05 µg/mL), followed by the analogs A, E, B, F and C, respectively. This demonstrated that the hydroxylation at C4' on the phenolic ring was the molecular modification with most importance for the observed activity.

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“…[46], with modifications. This method relies on the inhibition of tyrosinase in the presence of its substrate tyrosine, interrupting the synthesis of melanin.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Resveratrol Analogs on Mushroom Tyrosinase Activity

et al. 2012

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“…Vários produtos naturais, especialmente substâncias com atividade antifúngica, antiviral, antibacteriana e tripanosomicida, foram avaliados por este método e demonstraram uma relação signicativa. É também um ensaio rápido, simples, prático e de baixo custo (LEITE et al, 2009 se novos agentes com esta ação, principalmente inibidores de enzimas envolvidas na melanogênese, como a tirosinase (MACRINI et al, 2009). Nas concentrações 100, 300 e 1000 µg/mL avaliadas de extrato bruto, não foi observada interferência sobre a atividade da tirosinase, pois os valores obtidos não foram signicativos quando comparados ao controle.…”

Section: Gráfico 1 -Porcentagem De Inibição Do Complexo Fosfomolibdênunclassified

PHYTOCHEMICAL STUDY AND BIOLOGICAL ACTIVITIES OF SUNFLOWER BUD (Helianthus annuus L.)

Mercali¹,

Hirota²,

Miyazaki³

et al. 2012

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O girassol é cultivado especialmente para produção do óleo e, recentemente, o broto de girassol passou a ser consumido por um grupo específico de pessoas, principalmente os vegetarianos. Devido ao aumento do uso destes brotos como alimento, este trabalho teve como objetivo investigar a composição fitoquímica e as atividades biológicas do broto de girassol. Para tanto, o extrato etanólico bruto e frações dos brotos foram submetidos aos ensaios de toxicidade frente à Artemia salina, atividade antioxidante pela formação do complexo fosfomolibdênico, atividade hemolítica e atividade sobre a enzima tirosinase. O ensaio de toxicidade demonstrou que as frações hexano e hidroalcoólica não apresentaram toxicidade. As amostras não causaram hemólise e o extrato etanólico bruto não apresentou atividade sobre a enzima tirosinase. O extrato bruto, a fração clorofórmio e acetato de etila revelaram notável capacidade antioxidante.

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“…Devido a grande biodiversidade do cerrado brasileiro, os agentes podem ser encontrados e, eventualmente, usados em cosméticos e produtos farmacêuticos (Macrini et al, 2009).…”

Section: Introductionunclassified

Avaliação da atividade antioxidante e inibição da tirosinase do extrato das folhas do jatobá (Hymenaea stigonocarpa Mart. ex Hayne)

Miranda

Castro

Silvério

2014

Rev. bras. plantas med.

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Determination of total phenol, antioxidant activity and inhibition by tyrosinase extract of jatobá (Hymenaea stigonocarpa Mart. ex Hayne) leaves. The jatobá species, also known as Brazilian cherry, are traditionally used for the treatment of various diseases. Chemotaxonomic studies have described the Hymenaea genus as a potential source of phenolic compounds, tannins and flavonoids, which have antioxidant activity, thus being potential inhibitors of tyrosinase, which is the enzyme responsible for skin pigmentation defects. There are approximately 15 species in the genus Hymenaea of which 13 are found in Brazil. This study was conducted to evaluate the phenols, the antioxidant activity, the ability to chelate copper ions and the ability to inhibit tyrosinase of the extract of the H. Stigonocarpa leaves. The plant material (leaves) was harvested from trees in the savannah (Brazilian Cerrado) area of environmental preservation of the FESURV campus -University of Rio Verde -state of Goiás, dried in a forced circulation oven at 42° C for 2 days and subjected to extraction with hexane (hexane crude extract) and extraction with absolute ethanol (ethanol crude extract). When determining the phenolic content performed with the Folin-Ciocalteu reagent, we found that the crude ethanol extract (CEE) presented the highest concentration (235.7 mg gallic acid equivalent per gram of CEE). In the evaluation of radical scavenging activity, using the DPPH free radical, the ethanol extract again showed higher antioxidant activity (IC50 = 19 ± 0.1 ppm). For the procedure for chelation of copper ions, the crude ethanol extract tested showed no such ability. For the process of inhibiting the tyrosinase enzyme, the crude ethanol extract tested after 30 and 60 minutes presented inhibition of 38 and 48%, respectively.

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Extracts from Amazonian plants have inhibitory activity against tyrosinase: an in vitro evaluation

Cited by 20 publications

References 15 publications

Inhibitory Effects of Resveratrol Analogs on Mushroom Tyrosinase Activity

Inhibitory Effects of Resveratrol Analogs on Mushroom Tyrosinase Activity

PHYTOCHEMICAL STUDY AND BIOLOGICAL ACTIVITIES OF SUNFLOWER BUD (Helianthus annuus L.)

Avaliação da atividade antioxidante e inibição da tirosinase do extrato das folhas do jatobá (Hymenaea stigonocarpa Mart. ex Hayne)

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