Extraction optimisation, antioxidant activity and inhibition on α‐amylase and pancreatic lipase of polyphenols from the seeds of Syzygium cumini

Abstract

Summary The objectives of this study were to determine the optimal extraction conditions of polyphenols from Syzygium cumini seeds by response surface methodology and investigate their antioxidant activity and inhibition on α‐amylase and pancreatic lipase. As results, the optimal extraction conditions in the ultrasonic extraction process which maximised total polyphenols content, minimised the IC50 values of α‐amylase and pancreatic lipase were determined as follows: extraction time 60 min, ethanol concentration 63% and solvent/solid ratio 44 mL g−1. The main phenolic compounds in partially purified fraction of Syzygium cumini seeds were catechin, epicatechin, kaempferol, gallic, 5‐caffeoylquinic, caffeic and ferulic acids. In addition, the partially purified fraction inhibited 87.66 ± 5.55 and 86.61 ± 3.15% of α‐amylase and pancreatic lipase, respectively. The results suggested that Syzygium cumini seeds could be explored as a natural antioxidant and could be used as a source of highly antidiabetic and anti‐obesity bioactive compounds.

Production and Characterization of Sodium Alginate/Gum Arabic Based Films Enriched with Syzygium cumini Seeds Extracts for Food Application

“…The obtained SCSE was analyzed by HPLC and the results were recorded as our previous report [18]. The main phenolic constituents were epicatechin, kaempferol, catechin, gallic, 5-caffeoylquinic, caffeic and ferulic acids.…”

“…The strongest effect to scavenge the tested free radicals mainly attributed to the presence of polyphenols inside films. The SCSE mainly possessed epicatechin, catechin, kaempferol, 5-caffeoylquinic, caffeic, gallic and ferulic acids and these polyphenols might be the main reason for scavenging of DPPH and ABTS radicals [18]. However, the control films without SCSE showed ability to reduce the tested stable radicals and this might be explained by the reason that alginate polymer has antioxidant effect and this mainly increases by breaking the polymer chain [39,40].…”

“…The seeds were dried at 50 °C for 12 h and ground to a fine powder. The extraction method was performed based on the optimization conditions reported by our previous method [18] to afford the target extract.…”

Valorization of red raspberry (Rubus idaeusL.) seeds as a source of health beneficial compounds: Extraction by different methods

“…One of the most important effects of ellagic acid is the anticancer effect and one of the main reasons for the anticancer activity is the modulation of the toxin metabolism and prevention of carcinogenesis induced by these compounds (Seeram et al., 2006). Further, polyphenolic compounds, such as ellagic acid, have a strong effect on the inhibition of the α‐amylase and pancreatic lipase, which can be helpful in solving diabetic and obesity problems (Abdin et al., 2019). Ellagic acid also inhibits the activity of aflatoxin and in that way exerts its anti‐microbiological properties (Soni, Lahiri, Chackradeo, Bhide, & Kuttan, 1997).…”

Enhanced the entrapment and controlled release of Syzygium cumini seeds polyphenols by modifying the surface and internal organization of Alginate‐based microcapsules

“…According to Abdin et al. (2019), the fruits were washed and sunny dried for 2 days and then, transferred to microwave oven for half minute to extract seeds easily. The seeds were oven dried at 50°C for 12 hr.…”