Extraction of Tannin from Acacia (Acacia mangium) Bark and its use as a Feed Additive for Protecting in vitro Ruminal Degradation of Tofu Dregs

Sujarnoko, Tekad Urip Pambudi; Ridwan, Roni; Nahrowi, Nahrowi; Jayanegara, Anuraga

doi:10.17582/journal.aavs/2020/8.7.761.765

Cited by 3 publications

(7 citation statements)

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“…Regarding the CTC, among all tested extracts, the EtOAc extract was the only one which showed any significant content (Table 2). The CTC in this study, which was around 8 mg of catechin equivalents per dry residue mg CE/g dr, which was higher compared to the content found by Sujarnoko et al [16]. They found a CTC in the Acacia mangium bark, which did not exceed 2 mg CE/g dr. Statistically, a good positive correlation was found between TPC and CTC (r = 0.58) (Table 3).…”

Section: Yield Extraction Total Phenolic Content (Tpc) and The Reduci...contrasting

confidence: 81%

Copaifera mildbraedii Desf.: Phytochemical Composition of Extracts, Essential Oil, and In Vitro Biological Activities of Bark

Namkona,

Rahmani,

Worowounga

et al. 2024

Plants

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Copaifera mildbraedii Desf. is an evergreen tree with an umbrella-like crown. It is distributed from south-eastern Nigeria eastward to the Central African Republic (CAR). The aim of this study was to assess the chemical composition and biological activities of C. mildbraedii bark, as well as the chemical composition of the essential oil. Ethyl acetate (EtOAc) and methanol (MeOH) extracts showed a high total phenolic content (TPC) (149.9 and 148.8 mg GAE/g dry residue (dr), respectively), which was related to good antioxidant activity (DPPH) with an IC50 of 21.2 and 12.9 µg/mL, respectively. High-performance liquid chromatography coupled with diode array detector (HPLC-DAD) analysis revealed seven phenolic compounds with myricitrin (13.3 mg/g dr) and 2,4-dihydroxy-3,6-dimethyl benzoic acid (30.7 mg/g dr) as major compounds, while gas chromatography-mass spectrometry (GC-MS) analysis enabled detection of 13 volatile compounds (3 before and 10 after derivatization). Thirty compounds were identified in the essential oil, which corresponds to 65% of all identified compounds. Among the latter, E,E-farnesylacetone and γ-gurjunene were considered as major compounds (8.08 and 10.43%, respectively). The EtOAc extract showed a potent potential, simultaneously, against anti-acetylcholinesterase (AChE), anti-15-lipoxygenase (15-LOX), anti-xanthine oxidase (XOD), and cytotoxic (OVCAR) activities, whereas cyclohexane (CYHA) and dichloromethane (DCM) extracts showed a cytotoxic effect with high percentages of inhibition (95.2%).

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Section: Yield Extraction Total Phenolic Content (Tpc) and The Reduci...contrasting

confidence: 81%

Copaifera mildbraedii Desf.: Phytochemical Composition of Extracts, Essential Oil, and In Vitro Biological Activities of Bark

Namkona,

Rahmani,

Worowounga

et al. 2024

Plants

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“…There are deaminating microorganisms in the rumen that can convert feed proteins into ammonia and carbon groups [3]. The rumen can convert non-protein nitrogen compounds into microbial proteins that can be consumed by the host [18].…”

Section: In Vitro Effect Of Temulawak Powder Dosage On Rumen Metabolismmentioning

confidence: 99%

“…Curcumin, which is present in Temulawak powder, has antimicrobial properties [2]. Tannin in temulawak also can bind protein and decrease the deamination proses [3]. Consequently, temulawak powder can act as a protein-protecting agent during the tofu dreg silage process.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Temulawak (Curcuma xhantorrhiza) Dose on Tofu Dreg Plus Complete Feed Silage With Methane Relative Mitigation: In-Vitro

Urip Pambudi Sujarnoko,

Saputra Lim,

Budiono

et al. 2023

E3S Web Conf.

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Temulawak is an antimicrobial herb that can preserve the proteins in the feed from deamination and decay during the silage period. Tofu dregs are a feed ingredient that is difficult to preserve because they have high water content and protein. Temulawak powder may decrease deamination during the silage process because it has the ability as an antimicrobe and protein binder. Temulawak can reduce methane emissions by diminishing the number of protozoa that serve as hosts for methanogenic archaea. Temulawak with a harvest age of 3 months after planting, then dried at 65°C to constant weight, grounded, and tested on mix ration silage at doses of 0%, 0.25%, 0.5%, 0.75 %, and 1% from feed dry matter (DM). The experiment was carried out four times using a complete random design and in vitro silage was evaluated in the following 14 days. The results indicated that adding temulawak would reduce ammonia production with p<0.01, significantly increase the crude protein content of silage P<0.05 and decrease rumen ammonia P<0.01. The treatment increased total volatile fatty acid (VFA) production P<0.01), and reduced methane level P<0.01. The methane relative to dry matter (DM), organic matter, and total gas revealed a significant decrease with P<0.05. The 0.25% treatment level had the lowest total methane concentration and relative. Concluded that the optimal dose of temulawak addition to complete feed plus tofu dreg silage is 0.25% from DM.

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“…Salah satu senyawa yang mampu dan banyak terdapat di daerah tropis adalah tanin. Tanin merupakan senyawa yang mampu memproteksi protein dari proses degradasi oleh mikroba (Sujarnoko et al 2020). Setiap tanaman memiliki tanin yang spesifik (EFSA 2004).…”

Section: Pendahuluanunclassified

“…Tanin terhidrolisis memiliki kestabilan yang rendah sehingga mudah dipecah menjadi asam fenol dan gula sederhana (Garlack et al 2018). Kestabilan CT yang tinggi dapat dimanfaatkan sebagai by-pass nutrien yang baik untuk ternak ruminansia (Sujarnoko et al 2020). Hal ini dapat terlihat dari penurunan jumlah mikroba yang tinggi saat pemberian terhadap rumen ternak (Jayanegara et al 2019).…”

Section: Pendahuluanunclassified

PENGARUH PEMBERIAN EKSTRAK TANIN ASAL CHESNUT PADA SISTEM KEKEBALAN DARAH TIKUS (Rattus norvegicus)

Sujarnoko¹,

Firmansyah

2022

JSTSV

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This study aimed to observe effect of tannin from chestnut extract on the immune system of rats. Tannin in chestnut easily hydrolyzed into sugar and phenol groups, this is very different with largely tannin from other plants which are more condensed. The phenol group in chestnut tannins increased the rat immune, because it is capable of being an antioxidant and antibacterial in the digestive and blood metabolism of rats. In this observation used 8 male adult rats with age more than 90 days. The first treatment is rat feeder without chesnut extract and the second treatment is rat feeder with 0.25% chestnut extract from dry matter of feed. This research was being tested with independent T-test method with 4 replication. The results of the study that the addition of 0.25% chestnut extract from dry matter had no effect on consumption and increased body weight, increased cell capacity of rats p<0.05, there was an increase in active cells p<0.01, and increased bacterial mortality of Salmonella pullorum in the blood test. ABSTRAKPenelitian ini bertujuan mengamati pengaruh pemberian tanin dari ekstrak chesnut terhadap daya tahan tubuh tikus. Tanin pada chesnut merupakan senyawa yang mudah terhidrolisis menjadi gugus gula dan fenol, hal ini sangat berbeda dengan tanin pada beberapa tumbuhan yang lebih bersifat terkondensasi. Gugus fenol pada tanin chesnut diperkirakan dapat meningkatkan daya tahan tubuh tikus, karena mampu menjadi antioksidan dan antibakteri di dalam metabolisme pencernaan dan darah tikus. Pengamatan pada penelitian ini dilakukan pada 8 ekor tikus dewasa dengan umur lebih dari 90 hari yang diberi perlakuan berbeda. Perlakuan pertama adalah pemberian pakan tanpa ekstrak tanin chesnut dan perlakuan kedua adalah tikus dengan pakan yang ditambah ekstrak tanin chesnut sebanyak 0,25% dari bahan kering pakan. Rancangan analisis pada penelitian ini menggunakan uji T independent dengan 4 ulangan pada setiap perlakuan. Hasil penelitian penambahan 0,25% ekstrak chesnut dari bahan kering tidak berpengaruh terhadap konsumsi dan peningkatan bobot ternak, kapasitas sel tikus meningkat p<0,05, terjadi penambahan sel aktif p<0,01, dan meningkatnya kematian baketri Salmonella pullorum pada uji darah tikus yang dilakukan uji tantang.

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Extraction of Tannin from Acacia (Acacia mangium) Bark and its use as a Feed Additive for Protecting in vitro Ruminal Degradation of Tofu Dregs

Cited by 3 publications

References 0 publications

Copaifera mildbraedii Desf.: Phytochemical Composition of Extracts, Essential Oil, and In Vitro Biological Activities of Bark

Copaifera mildbraedii Desf.: Phytochemical Composition of Extracts, Essential Oil, and In Vitro Biological Activities of Bark

The Effect of Temulawak (Curcuma xhantorrhiza) Dose on Tofu Dreg Plus Complete Feed Silage With Methane Relative Mitigation: In-Vitro

PENGARUH PEMBERIAN EKSTRAK TANIN ASAL CHESNUT PADA SISTEM KEKEBALAN DARAH TIKUS (Rattus norvegicus)

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