Extraction of carotenoids from Phaffia rhodozyma: A comparison between different techniques of cell disruption

Michelon, Mariano; Borba, Thais de Matos de; Rafael, Ruan da Silva; Burkert, Carlos André Veiga; Burkert, Janaína Fernandes de Medeiros

doi:10.1007/s10068-012-0001-9

Cited by 97 publications

(67 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was incubated at 25 °C and 150 rpm for 48 h or for the time required for the cell count to be at least 10 8 cells/mL, counted by a Neubauer chamber (Optik Labor, Lancing, UK) (9). Bioproduction tests were conducted on an orbital shaker (model TE 425; Tecnal, Piracicaba, Brazil) with controlled stirring rate and temperature.…”

Section: Culture Conditions and Production Of Carotenoidsmentioning

confidence: 99%

“…Therefore, the yeast Phaffi a rhodozyma (also referred to as Xanthophyllomonas dendrorhous) stands out as a natural source of carotenoids (8)(9)(10)(11)(12) due to its ability to assimilate diff erent carbon sources as substrates, heterotrophic metabolism, relatively fast growth rate, ability to reach high cell density and nutrient quality and safety as a food additive. Besides, it has been certifi ed as a GRAS (Generally Recognized as Safe) microorganism.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

Silva¹,

Borba²,

Kalil³

et al. 2016

Food Technol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

SummarySearch for naturally grown food has stimulated the biotechnological production of carotenoids. Therefore, the use of the yeast Xanthophyllomonas dendrorhous has been researched due to its abilities to assimilate diff erent sources as substrates and to produce high amounts of carotenoids. Furthermore, alternative sources have been used as the culture medium to reduce costs and environmental impact. A potent carotenoid is astaxanthin in view of its health-promoting and antioxidative properties. It consists of diff erent geometrical isomers with trans and cis confi guration. In X. dendrorhous this carotenoid is mostly found in the trans form, but cis isomers can also be found. Carotenoid production was investigated in culture medium containing by-products such as raw glycerol (from biodiesel) and parboiled rice effl uent. The eff ects of the culture medium components on biomass concentration and specifi c and volumetric productions of carotenoids were verifi ed by the Plackett -Burman design. Cultivations were carried out with yeast Xanthophyllomonas dendrorhous NRRL Y-17268 at 25 °C and 150 rpm for 168 h. In this study, maximum production of carotenoids was obtained under the following conditions (in g/L): raw glycerol 10, glucose 10, yeast extract 10, malt extract 10 and peptone 1 at pH=6. Resulting specifi c and volumetric productions of carotenoids were 326.8 and 4.1 μg/g, respectively.

show abstract

Section: Culture Conditions and Production Of Carotenoidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

Silva¹,

Borba²,

Kalil³

et al. 2016

Food Technol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The suspension was maintained for 48 h at 25 °C in a rotary incubator at 150 rpm, and growth was monitored by counting in a Neubauer chamber (Michelon et al, 2012).…”

Section: Inoculummentioning

confidence: 99%

“…A significant increase in carotenoid production by various microorganisms has been observed with the use of the methodology of factorial design to evaluate different means of production, culture conditions, such as pH, temperature, aeration, and inoculum concentration, and recovery of these compounds (Ni et al, 2007;Liu;Wu, 2007;Gu et al, 2008;Ni et al, 2008;Valduga et al, 2011;Cabral et al, 2011;Michelon et al, 2012). In previous work conducted by our research group, the use of a culture medium containing rice parboiling wastewater (15 g L -1…”

Section: Introductionmentioning

confidence: 99%

RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma

et al. 2015

View full text Add to dashboard Cite

The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL -1 (259.1 μg g -1 ) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2 IV 6-2 fractional factorial design sequenced by a 2 4 central composite design). The conditions for maximizing the production of carotenoids were (g L -1) malt extract (16.25), peptone (8.75), sucrose (15) and rice parboiling wastewater (87.5), with a pH of 5 at 25 °C and 150 rpm for 144 h, which produced a concentration 5.3 μg mL -1 (628.8 μg g -1 ).Index terms: Biodye; submerged cultive; response surface methodology; yeast. RESUMOO interesse pela produção de carotenoides por fontes naturais é crescente, em decorrência da possibilidade de atuar como corante e sua capacidade antioxidante biológica potente. Neste estudo, avaliou-se o uso da água de parboilização do arroz, como substrato alternativo para a bioprodução de carotenoides, usando a levedura Phaffia rhodozyma que, como única fonte de nutrientes, foi promissora, alcançando 0,6 µg mL -1 (259,1 µg g -1

show abstract

“…Aos sobrenadantes foram adicionados 10 mL de solução de NaCl 20% (p.v -1 ) e 10 mL de éter de petróleo. Após a formação de duas fases a fase apolar foi filtrada com sulfato de sódio (Na 2 SO 4 ), dando origem aos extratos carotenogênicos (MICHELON, et al, 2012).…”

Section: Rompimento Celular E Obtenção Dos Extratos Carotenogênicosunclassified

INTERFERÊNCIA DO pH INICIAL E VOLUME DE MEIO NA PRODUÇÃO DE CAROTENOIDES POR Sporidiobolus pararoseus

Tavares¹,

Sá²,

Borba³

et al. 2015

Anais Do Congresso Brasileiro De Engenharia Química Em Iniciação Científica - Cobeq IC 2015

View full text Add to dashboard Cite

RESUMO -Fatores como composição do meio, temperatura, aeração e pH podem interferir na produção de microbiana de carotenoides. Em vista disso, o presente trabalho teve como objetivo avaliar o efeito do pH inicial (3 à 5) e a relação de volume de meio para volume de reator (3:10 à 7:10) no cultivo em frascos agitados para produção de carotenoides por S. pararoseus. Na faixa estudada, apenas a variável pH presentou um efeito significativo (p<0,05) sobre a resposta carotenoides totais. O valores máximo de carotenoides totais alcançados foram de 204,7 (ensaio 2) a 463,9 µg/L (ensaio 1) e para biomassa de 4,6 (ensaio 4) a 13,6 g/L (ensaio 1), sendo que os menores valores na produção de carotenoides e de biomassa ocorreram nos ensaios com pH 5,0 (nível +1). A produção em pH próximo a 3 levou a uma aumento e aproximadamente 56% na produção de carotenoides e 66% na biomassa foi produzida nesse pH do que em comparação ao pH 5. INTRODUÇÃOOs carotenoides estão amplamente distribuídos na natureza, apresentando papel de destaque na fotossíntese e fotoproteção de sistemas vegetais e como precursores de vitamina A nos seres humanos. Além de funções nos sistemas biológicos, apresentam também grande importância para a indústria farmacêutica na elaboração de cosméticos e como aditivo alimentar na indústria de alimentos (DAMODARAN, et al., 2010).Diversos estudos demonstram que a composição do meio empregado pode interferir na bioprodução desses pigmentos, além disso, outras condições externas do cultivo como temperatura, agitação, aeração e pH também levam a alterações como variedade e quantidade de carotenoides produzidos pelo micro-organismo (RIBEIRO, et al., 2011;SCHWARTZ, 2010).Em vista do apresentado o presente trabalho possui como objetivo avaliar a interferência do pH inicial e a relação Vmeio:Vreator na produção de carotenoides por Sporidiobolus pararoseus em frascos agitados MATERIAL E MÉTODOS

show abstract

Extraction of carotenoids from Phaffia rhodozyma: A comparison between different techniques of cell disruption

Cited by 97 publications

References 31 publications

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

Raw Glycerol and Parboiled Rice Effluent for Carotenoid Production: Effect of the Composition of Culture Medium and Initial pH

RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma

INTERFERÊNCIA DO pH INICIAL E VOLUME DE MEIO NA PRODUÇÃO DE CAROTENOIDES POR Sporidiobolus pararoseus

Contact Info

Product

Resources

About