Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae)

The biological activity and chemistry of the southern African Combretaceae

“…The solvents used in the study were compared by allocation of points on an arbitrary five point weighted scale, and acetone achieved the best results (Eloff, 1998b). In investigation of many species using different extractants subsequently, acetone was always one of the best extractants (Kotze and Eloff, 2002).…”

“…Combretum microphyllum was used to prepare extracts of dried ground leaves using solvents with different polarities, including hexane, carbon tetrachloride, di-isopropylether, ethyl ether, methylene dichloride, tetrahydrofuran, acetone, ethanol, ethyl acetate, methanol and water (Kotze and Eloff, 2002). Thin layer chromatography (TLC) and a microplate serial dilution method were used to determine chemical composition and antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis of extracts, respectively.…”

“…The use of different extractants to extract leaf material of Combretum microphyllum was examined (Kotze and Eloff, 2002). It was found that there was a surprising similarity in the chemical composition of the non-polar components of extracts using extractants of widely varying polarity, and belonging to different selectivity groups.…”

Antifungal activities of six South African Terminalia species (Combretaceae)

“…The TLC plates were developed under saturated conditions with one of the three eluent systems developed in our laboratory that separates components of Combretaceae extracts well, i.e., ethyl acetate/methanol/water (40:5.4:5): [EMW] (polar/neutral); chloroform/ethyl acetate/formic acid (5:4:1): [CEF] (intermediate polarity/acidic); benzene/ethanol/ammonia hydroxide (90:10:1): [BEA] (non-polar/basic) (Kotze and Eloff, 2002).…”

Inhibitory properties of selected South African medicinal plants against Mycobacterium tuberculosis

“…Several studies have shown acetone to be an efficient extractant of diverse bioactive compounds (Eloff, 1998;Kotze and Eloff, 2002;Madamombe and Afolayan, 2003;Asres et al, 2006) that may act in synergy and produce a greater antimycobacterial activity, resulting in high susceptibility patterns with low MIC values observed in this study. Flavonoids and steroids have been extracted using acetone (Eloff, 1998).…”