Inhibitory properties of selected South African medicinal plants against Mycobacterium tuberculosis

Green, Ezekiel; Samie, Amidou; Chikwelu, Larry; Ndip, Roland N.

doi:10.1016/j.jep.2010.04.033

Cited by 88 publications

(81 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They identified three species, S. verbenaca, S. radula and S. dolomitica, which exhibited MIC value at 0.10 mg/mL and which also showed antibacterial activity. Green et al (2010) reported on the activities of acetone extracts of four plants, while Berchemia discolor showed efficacy at MIC 12. 5µg/mL, on H37Ra and 10.5µg/mL on the clinical isolate; the others (Bridelia micrantha, Warbugia salutaris, and Terminalia sericea) showed efficacy at 25µg/mL on both H37Ra and clinical isolate.…”

Section: Discussionmentioning

confidence: 99%

Antimycobacterial Activity Some Different Lamiaceae Plant Extracts Containing Flavonoids and Other Phenolic Compounds

Aşkun¹,

Tümen²,

Satıl³

et al. 2012

Understanding Tuberculosis - New Approaches to Fighting Against Drug Resistance

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Antimycobacterial Activity Some Different Lamiaceae Plant Extracts Containing Flavonoids and Other Phenolic Compounds

Aşkun¹,

Tümen²,

Satıl³

et al. 2012

Understanding Tuberculosis - New Approaches to Fighting Against Drug Resistance

View full text Add to dashboard Cite

“…Moreover, an acetone extract of the leaf was reportedly less active against two Mycobacterium tuberculosis strains, with MIC of ˃0.1 mg/ml compared to the reference drug (isoniazid) which exhibited a MIC of 0.0001 and 0.005 mg/ml (Green et al, 2010). The result of ˃0.1 mg/ml is not useful information as it cannot be compared to information from other authors.…”

mentioning

confidence: 91%

Securidaca longipedunculata Fresen (Polygalaceae): A review of its ethnomedicinal uses, phytochemistry, pharmacological properties and toxicology

Mongalo

McGaw

Finnie

et al. 2015

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacologicalrelevance: Securidaca longipedunculata Fresen (Polygalaceae) is a multi-purpose plant with a long history of use in African traditional medicine to treat various sexually transmitted infections, hernias, coughs, fever, ascariasis, constipation, headaches, rheumatism, stomach ache, malaria, tuberculosis, pain, epilepsy, pneumonia, skin infections, and it is also used as an aphrodisiac for men. The current paper provides an overview of the present phytochemistry, toxicology, ethnomedicinal uses and pharmacological properties of S. longipedunculata. Materials and methods:The information reported in this paper was collected from a literature search using various computerised databases including ScienceDirect, Moreover, the pharmacokinetic properties of extracts and compounds of the species need to be explored as there is insufficient data available on these aspects.

show abstract

“…According to previous research done, some plants have shown significant activity against Mycobacterium in in vitro studies, with a minimum inhibitory concentration (MIC) of lower than 50ug/ml. These plants include; Berchemia discolor with an MIC of 12.5 µg/ml, Warbugia salutaris (25 µg/ml), Terminalia sericea (25 µg/ml), Bridelia micrantha, and Diospyros mespiliformis (12.5 µg/ml) (Green et al, 2010;Mahapatra et al, 2007). Euclea natalensis, a plant belonging to the Ebenaceae family has been used by multiple communities in southern Africa including, Tsonga, Shangaan, Venda and the Zulu tribes.…”

Section: Introductionmentioning

confidence: 99%

In vitro and In vivo antimycobacterial, hepatoprotective and immunomodulatory activity of Euclea natalensis and its mode of action

Lall

Kumar

Meyer

et al. 2016

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacological relevance: The Natal gwarri or Natal ebony (Euclea natalensis A.DC.) is a deciduous tree found widespread throughout southern Africa, especially in Kwazulu-Natal and the southern cost. It has been widely used by indigenous communities such as the Zulus, Tsongas and Vendas for symptoms related to tuberculosis (TB). The decoctions made from the plant parts are administered for chest diseases to treat complications such as chest pains, bronchitis, pleurisy and asthma. TB is prevalent in immune-compromised patients and it is evident that TB-drugs cause hepatotoxicity. The objective of the present study was therefore to evaluate the antimycobacterial activity of the 2 ethanolic extract of E. natalensis against TB and its hepatoprotective and immunomodulatory activities. Materials and methods:The antimycobacterial, antioxidant, hepatoprotective, immunomodulatory activity and cytotoxicity of the ethanolic extract of the shoots of E. natalensis were determined in vitro. The mechanism of action of the antituberculosis activity was determined by investigating the inhibitory effect on mycothiol disulfide reductase enzyme. Furthermore, the acute, sub-acute toxicity (50-2000 mg/kg) and antimycobacterial effect (300 mg/kg) of E. natalensis shoot extract were investigated in Balb/c mice.Hepatoprotective activity of the extract (50-150 mg/kg) was evaluated on isoniazid and rifampicin (50 mg/kg; i.p.) induced hepatic damage in a rat model. Results:The minimum inhibitory concentration of the extract was found to be 125 µg/ml against Mycobacterium tuberculosis. The extracts fifty percent inhibitory concentration (IC 50 ) against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was found to be 22.55 µg/ml. The plant showed a hepatoprotective effect (50% at 12.5 µg/ml) and the ability to increase Thelper 1 cell cytokines; Interleukin 12, Interleukin 2 and Interferon α by up to 12 fold and the ability to decrease the T-helper 2 cell cytokine Interleukin 10 4 fold when compared to baseline cytokine production. No cellular toxicity was observed in primary peripheral blood mononuclear cells (PBMC's) and two secondary cell lines; U937 monocytes and Chang liver cells (a derivative of the HepG2 cell line). During mechanistic studies, the extract showed a 50% inhibition of mycothiol reductase activity at 38.62 µg/ml. During the acute and subacute studies, E. natalensis exhibited no toxic effect and the fifty percent lethal dose (LD 50 ) was established to be above 2000 mg/kg. The extract was able to reduce the mycobacterial load (1.5-fold reduction) in infected mice. Isoniazid and rifampicin caused significant hepatic damage in rats, and the extract was able to reduce the toxicity by 15% and 40% at 50 and 150 mg/kg respectively. Conclusion:The present study supports the traditional usage of the plant against tuberculosis symptoms. The study showed the ability of E. natalensis shoot extract to inhibit mycobacterial growth, stimulate an appropriate immune response and have a hepatic protective effect. Due to the ...

show abstract

Inhibitory properties of selected South African medicinal plants against Mycobacterium tuberculosis

Cited by 88 publications

References 46 publications

Antimycobacterial Activity Some Different Lamiaceae Plant Extracts Containing Flavonoids and Other Phenolic Compounds

Antimycobacterial Activity Some Different Lamiaceae Plant Extracts Containing Flavonoids and Other Phenolic Compounds

Securidaca longipedunculata Fresen (Polygalaceae): A review of its ethnomedicinal uses, phytochemistry, pharmacological properties and toxicology

In vitro and In vivo antimycobacterial, hepatoprotective and immunomodulatory activity of Euclea natalensis and its mode of action

Contact Info

Product

Resources

About