Antifungal activities of six South African Terminalia species (Combretaceae)

Masoko, Peter; Picard, J.; Eloff, Jacobus Nicolaas

doi:10.1016/j.jep.2005.01.061

Cited by 180 publications

(108 citation statements)

References 12 publications

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“…The minimum inhibitory concentration (MIC) of the crude acidified 70% acetone extracts and different fractions against four bacterial strains and three fungal strains were determined using a broth microdilution technique (Eloff, 1998) for the bacterial assay and modified for fungal assay by Masoko et al (2005).…”

Section: Minimum Inhibitory Concentration Evaluationmentioning

confidence: 99%

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Ahmed

McGaw

Elgorashi

et al. 2014

Journal of Ethnopharmacology

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Graphical abstractPhenolic-enriched extracts of four Combretum species namely Combretum bracteosum, Combretum padoides, Combretum vendae and Combretum woodii used in South African traditional medicine to treat various ranges diseases were evaluated against some diarrhoeal aetiologies. All the crude extracts and their fractions of varied polarities had good antimicrobial (non-polar fractions) or good antioxidant (polar fractions) activity. The crude extract of all the plant species were cytotoxic to Vero cell lines but did not lead to COX enzyme inhibition. Phenolic-enriched extractsFour Combretum species traditionally used for treating infectious and gastrointestinal complications in southern Africa In vitro assays: antioxidant, anti-inflammation, antimicrobial, cytotoxicity AbstractEthnopharmacological importance: Infections and gastrointestinal (GIT) disorders such as diarrhoea causes many problems in human health and animal production. Many Combretum species are used in traditional medicine to treat various diseases by rural people in Africa and Asia. Much of the work done to date on some species to validate their ethnopharmacological use was on the non-polar or intermediate polarity components. Many species are yet to be studied against relevant disease parameters using more polar extracts. Aims:The polar components were extracted and fractionated by solvent-solvent fractionation to yield fractions of different polarities. The activity of these fractions on different parameters that could be involved in infectious and gastrointestinal track (GIT) disorders was investigated. The cytotoxic activities of the extracts were also determined to evaluate the potential of these extracts to combat diarrhoea in production animals. Materials and Methods:Phenolic-enriched leaf extracts of Combretum bracteosum (Cob), Combretum padoides (Cop), Combretum vendae (Cov) and Combretum woodii (Cow) were obtained by extracting with a mixture of 70% acetone acidified with 1% HCl and n-hexane. The extract was sequentially treated by solvent-solvent fractionation with dichloromethane, ethyl acetate, and butanol to yield fractions with a large variation in polarity. The phenolic constituents of the extracts and fractions were determined using standard procedures. The antioxidant activities were determined using various standard methods. The minimum inhibitory concentrations (MICs) of the crude extracts and fractions against four bacterial and three fungal strains were assessed with a microplate serial dilution method.Cyclooxygenase (COX) and lipoxygenase (LOX) enzyme inhibitory assays and cytotoxicity studies against Vero cells were also carried out.Result: Some of the fractions had much higher antioxidant activity than the positive controls. The average EC 50 values of the extracts for the DPPH and ABTS antioxidant assays were 0.21-12 µg/ml (Cop), 0.25-16 µg/ml (Cov), 0.33-9.41 µg/ml (Cow) and 4.97-85 µg/ml (Cob) respectively while the mean EC 50 values for the positive controls ascorbic acid and trolox were 1.28-1.51 and 1.02-1.19 ...

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Section: Minimum Inhibitory Concentration Evaluationmentioning

confidence: 99%

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Ahmed

McGaw

Elgorashi

et al. 2014

Journal of Ethnopharmacology

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“…Various extracts from plants are traditionally used to protect crops and seeds (Shai et al, 2009). Some of these extracts and other metabolites as flavonoides showed significant antifungal activity than standards as fluconazol (Masoko et al, 2005;Orhan et al, 2010). Then, the significant antifungal activity of S. dulcis could be helpful for sanitary purpose in humans, plants and animals.…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative effect of <i>Scoparia dulcis</i> L. against bacterial and fungal strains

Coulibaly¹,

Konaté²,

Youl³

et al. 2013

Int. J. Bio. Chem. Sci

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Scoparia dulcis L. was sequentially extracted with hexane, chloroform and methanol and soaked with aqueous-acetone (80%) to check for its antimicrobial activities against five bacterial and four fungal strains. 250 µg of each extract loaded on a whatman paper disc exhibited significant antimicrobial activities on all the fungus and against Gram-negative and Gram-positive bacteria. Therefore, P. mirabilis is less sensitive to all the extracts while B. cereus, a β-lactamase producer bacterium, was resistant to the activity of the polar methanol and aqueous-acetone extracts. By the microdilution method, the most active extracts were chloroform extract on B. cereus with minimal inhibitory concentration (MIC) of 1.56 mg/ml and Aqueous-acetone extract on S. typhimurium (MIC = 1.56 mg/ml); the antifungal activity was strongest for hexane extract (MIC = 6.25 mg/ml) on both A. niger and P. roquefortii.

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“…Since then it has also been used to determine antifungal activity (Masoko et al, 2005) and drug susceptibility for mycobacteria (Gomez-Flores et al, 1995). The tetrazolium salt is converted to a coloured formazan salt in the presence of actively dividing cells.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of two biflavonoids from Garcinia livingstonei leaves against Mycobacterium smegmatis

Kaikabo

Eloff

2011

Journal of Ethnopharmacology

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Amentoflavone and 4′ monomethoxy amentoflavone were previously isolated from Garcinia livingstonei leaves. These compounds had good activities (MIC 6 and 8 μg/ml) against some nosocomial bacteria. In this study, the activity of these purified compounds were tested against fast-growing non-pathogenic Mycobacterium smegmatis. Amentoflavone was the most active compound, with an MIC of 0.60 ± 0.70 mg/ml. The MIC of 4′ monomethoxy amentoflavone and the positive control isoniazid against Mycobacterium smegmatis were similar 1.40 ± 1.56 and 1.30 ± 1.70 mg/ml respectively. Although, Mycobacterium smegmatis is a non-pathogenic fast growing mycobacterium the activities of these compounds may also be useful in combating infections by pathogenic Mycobacterium spp.

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Antifungal activities of six South African Terminalia species (Combretaceae)

Cited by 180 publications

References 12 publications

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Anti-proliferative effect of <i>Scoparia dulcis</i> L. against bacterial and fungal strains

Antibacterial activity of two biflavonoids from Garcinia livingstonei leaves against Mycobacterium smegmatis

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