Extraction, Detection, and Characterization of Rhamnolipid Biosurfactants from Microorganisms

Kumar, Rajesh; Das, Amar Jyoti

doi:10.1007/978-981-13-1289-2_2

Cited by 4 publications

(2 citation statements)

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“…Following the extraction or precipitation of the biosurfactant combination from the culture broth and subsequent dissolution in an organic phase, crystallization is used to purify the biosurfactant (Kumar and Das 2018). Because of its reduced solubility in the solvent, rhamnolipid crystallizes when a solvent such as hexane is added.…”

Section: Crystallizationmentioning

confidence: 99%

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Extraction, purification and applications of biosurfactants based on microbial-derived glycolipids and lipopeptides: a review

et al. 2021

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Microbial biosurfactants are sustainable alternatives for synthetic counterparts, and display high biodegradability, environmental compatibility, and high selectivity. While previous reports have focussed on production yield, little attention has been paid on downstream processing of biosurfactants, a critical step that controls quality. Here we review methods for biosurfactant downstream processing with focus on glycolipids and lipopeptidesglycolipids and lipopeptides. Solvent extraction, foam fractionation, and sweep floc coagulation allow to reach a purity of 55-70%. While distillation can be implemented prematurely, methods like ultrafiltration and chromatography are used as final processing to recover 90-95% of biosurfactant with 78-95% of purity. We present the cheapest and most efficient downstream techniques for industries to achieve acceptable product quality. This paper puts forth the environmental applications of biosurfactants. We review applications in treating hydrophobic organic pollutants and heavy metals in soils and water. The removal efficiency of biosurfactants reaches 96%. Biosurfactants in the detergent industry, microbial enhanced oil recovery, and soil washing are also discussed.

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Section: Crystallizationmentioning

confidence: 99%

“…The advantages of this process give high purity with high recovery and energy saving. Operating at a lower temperature and is suitable for packaging the biosurfactant but highly concentrated samples are required for this process (Kumar and Das 2018).…”

Section: Crystallizationmentioning

confidence: 99%