Recent progress and trends in the analysis and identification of rhamnolipids

Jiang, Jintao; Jin, Mingjie; Li, Xiaohua; Meng, Qin; Niu, Jian; Long, Xuwei

doi:10.1007/s00253-020-10841-3

Cited by 26 publications

(14 citation statements)

References 119 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oil displacement circle diameter can also indirectly evaluate the yield of biosurfactants producing bacteria (Marchut-Mikołajczyk et al, 2021 ). There is a positive linear correlation between the diameters of oil spreading circle and the concentration of biosurfactants (Zhao et al, 2016 ; Jiang et al, 2020 ). Using the established method, all the strains screened from the samples of oil reservoir produced water and oily sludge can reduce the surface tension of anaerobic culture broth to lower than 45 mN/m.…”

Section: Resultsmentioning

confidence: 99%

How to simply and efficiently screen microbial strains capable of anaerobic biosynthesis of biosurfactants: Method establishment, influencing factors and application example evaluation

Zhao

Wang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Microbial resources capable of anaerobic biosynthesis of biosurfactants are increasingly interested for their application in oxygen-deficient environments, such as in-situ microbial enhanced oil recovery and anaerobic bioremediation. How to simply and efficiently screen microbial strains capable of anaerobic biosynthesis of biosurfactants need be further studied in depth. In this study, an efficient and simple screening method was established based on the oil displacement characteristic of biosurfactants combined with the anaerobic culture technology using microplate assays. Strains whose anaerobic culture in microwells can form oil displacement circles with diameters larger than 10 mm were screened for scale-up culture in anaerobic tubes. The screened strains which can reduce the surface tension of anaerobic culture to lower than 45 mN/m were verified as positive strains. Using this screening method, eight positive strains and thirteen positive strains were screened from oil reservoir produced water and oily sludge, respectively. Through phylogenetic analysis, some screened strains were identified as Pseudomonas sp., Bacillus sp., and Enterobacter sp. This study also found that more microbial strains might be isolated after enrichment culture of environmental samples, whereas more microbial species would be isolated without enrichment. Suspension of environmental samples prepared with distilled water or normal saline had no significant effect. The established screening method is highly targeted and efficient for microbial strains capable of anaerobic biosynthesis of biosurfactants. The diameter of oil displacement circle is a reliable screening indicator. This study will contribute to explore more microbial resources which can anaerobically biosynthesize biosurfactants.

show abstract

Section: Resultsmentioning

confidence: 99%

How to simply and efficiently screen microbial strains capable of anaerobic biosynthesis of biosurfactants: Method establishment, influencing factors and application example evaluation

Zhao

Wang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…RL reached the market in the past decade; in 2019 nearly 25 000 tons were produced that represent almost 68 million US dollars, and the market size is expected to reach over USD 145 million by 2026 . Despite the huge market demand, the industrial applications and commercialization of RL are still limited by the relatively low level of its production . To build strains with enhanced RL production, several metabolic engineering strategies were developed, such as blocking the metabolic bypass that competed precursors with RL biosynthesis, containing exopolysaccharide (Psl and Pel) and polyhydroxyalkanoate (PHA) synthesis pathways .…”

mentioning

confidence: 99%

“…Other strategies like over/coexpressing key enzymes related to RL biosynthesis, such as the rhlAB - R and/or rmlBDAC operons, , the hemoglobin ( vhb ), and the outer membrane protein EstA . Although these attempts to some extent increased RL production, the lack of suitable chassis should be the main bottleneck for RL commercialization, as it greatly affects the production cost …”

mentioning

confidence: 99%

“…9 Despite the huge market demand, the industrial applications and commercialization of RL are still limited by the relatively low level of its production. 10 To build strains with enhanced RL production, several metabolic engineering strategies were developed, such as blocking the metabolic bypass that competed precursors with RL biosynthesis, containing exopolysaccharide (Psl and Pel) and polyhydroxyalkanoate (PHA) synthesis pathways. 11 Other strategies like over/coexpressing key enzymes related to RL biosynthesis, such as the rhlAB-R and/or rmlBDAC operons, 12,13 the hemoglobin (vhb), 14 and the outer membrane protein EstA.…”

mentioning

confidence: 99%

“…15 Although these attempts to some extent increased RL production, the lack of suitable chassis should be the main bottleneck for RL commercialization, as it greatly affects the production cost. 10 In this sense, rapid and high-throughput screening assays must be developed and established considering these high RL, especially high di-RL producers. Conventional screening for RL overproducing strains relies heavily on the CTAB-MB agar plates, because RL form an insoluble complex in the presence of cationic cetyltrimethylammonium bromide (CTAB), which when treated with methylene blue (MB) appears a specific dark blue.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers

Zhang

Cao

et al. 2022

ACS Synth. Biol.

View full text Add to dashboard Cite

Rhamnolipids (RLs) are biosurfactants with great economic significance that have been used extensively in multiple industries. Pseudomonas aeruginosa is a promising microorganism for sustainable RL production. However, current CTAB-MB based screening of RL-producing strains is time-consuming, labor-intensive, and unable to distinguish monoand di-RL. In this study, we developed a novel transcription-associated fluorescence-activated droplet sorting (FADS) method to specifically target the di-RL hyperproducers. We first investigated critical factors associated with this method, including the specificity and sensitivity for discriminating di-RL overproducers from other communities. Validation of genotype−phenotype linkage between the GFP intensity, rhlC transcription, and di-RL production showed that rhlC transcription is closely correlated with di-RL production, and the GFP intensity is responsive to rhlC transcription, respectively. Using this platform, we screened out ten higher di-RL producing microorganisms, which produced 54−208% more di-RL than the model P. aeruginosa PAO1. In summary, the droplet-based microfluidic platform not only facilitates a more specific, reliable, and rapid screening of P. aeruginosa colonies with desired phenotypes, but also shows that intracellular transcription-associated GFP intensity can be used to measure the yield of di-RL between populations of droplets containing different environmental colonies. This method also can be integrated with transposon mutation libraries to target P. aeruginosa mutants.

show abstract

Quantitative determination of rhamnolipid using HPLC‐UV through carboxyl labeling

Zhou

Miao

Yang

et al. 2023

Biotech and App Biochem

View full text Add to dashboard Cite

Rhamnolipid, as a low‐toxic, biodegradable and environmentally friendly biosurfactant, has broad application prospects in many industries. However, the quantitative determination of rhamnolipid is still a challenging task. Here, a new sensitive method for the quantitative analysis of rhamnolipid based on a simple derivatization reaction was developed. In this study, 3‐[3′‐(l‐rhamnopyranosyloxy) decanoyloxy] decanoic acid (Rha‐C10‐C10) and 3‐[3′‐(2′‐O‐α‐l‐rhamnopyranosyloxy) decanoyloxy] decanoic acid (Rha‐Rha‐C10‐C10) were utilized as the representative rhamnolipids. Liquid chromatography–mass spectrometry and high‐performance liquid chromatography‐ultra violet results showed that these two compounds were successfully labeled with 1 N1‐(4‐nitrophenyl)‐1,2‐ethylenediamine. There was an excellent linear relationship between rhamnolipid concentration and peak area of labeled rhamnolipid. The detection limits of the Rha‐C10‐C10 and Rha‐Rha‐C10‐C10 were 0.018 mg/L (36 nmol/L) and 0.014 mg/L (22 nmol/L), respectively. The established amidation method was suitable for the accurate analysis of rhamnolipids in the biotechnological process. The method had good reproducibility with the relative standard deviation of 0.96% and 0.79%, respectively, and sufficient accuracy with a recovery of 96%–100%. This method was applied to quantitative analysis of 10 rhamnolipid homologs metabolized by Pseudomonas aeruginosa LJ‐8. The single labeling method was used for the quantitative analysis of multiple components, which provided an effective method for the quality evaluation of other glycolipids with carboxyl groups.

show abstract

Recent progress and trends in the analysis and identification of rhamnolipids

Cited by 26 publications

References 119 publications

How to simply and efficiently screen microbial strains capable of anaerobic biosynthesis of biosurfactants: Method establishment, influencing factors and application example evaluation

How to simply and efficiently screen microbial strains capable of anaerobic biosynthesis of biosurfactants: Method establishment, influencing factors and application example evaluation

Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers

Quantitative determination of rhamnolipid using HPLC‐UV through carboxyl labeling

Contact Info

Product

Resources

About