Development, evaluation, and optimisation of downstream process concepts for rhamnolipids and 3-(3-hydroxyalkanoyloxy)alkanoic acids

Biselli, Andreas; Willenbrink, Anna-Lena; Leipnitz, Martin; Jupke, Andreas

doi:10.1016/j.seppur.2020.117031

Cited by 15 publications

(16 citation statements)

References 35 publications

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“…However, for industrial-scale processes, complex media are not suited. Furthermore, previous work within our consortium has demonstrated that the high protein/peptide content therein is detrimental for downstream processing ( Biselli et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…A pH value-dependent extraction screening of rhamnolipids from the cultivation supernatant was performed according to Biselli et al (2020) using ethyl acetate as organic solvent ( Schilling et al, 2016 ). Cells were removed via centrifugation.…”

Section: Methodsmentioning

confidence: 99%

“…The performance of the liquid–liquid extraction process was evaluated, according to Biselli et al (2020) , by the reduction of rhamnolipids from the aqueous phase, using the following equation:…”

Section: Methodsmentioning

confidence: 99%

“…Since Biselli et al (2020) showed that liquid–liquid extraction of rhamnolipids from the cultivation supernatant based on the LB medium using ethyl acetate is highly selective with purities of rhamnolipids in ethyl acetate close to 100 w%, purities will not be investigated at this point.…”

Section: Methodsmentioning

confidence: 99%

“…Protein/peptide content of aqueous samples in liquid–liquid extraction was determined by BCA Protein Assay (Thermo Fisher Scientific, Waltham, MA, United States) according to Biselli et al (2020) .…”

Section: Methodsmentioning

confidence: 99%

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Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production Using Pseudomonas putida

Tiso

Ihling

Kubicki

et al. 2020

Front. Bioeng. Biotechnol.

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E. coli HB101 pRK2013 Sm R , hsdR-M + , proA2, leuB6, thi-1, recA; harboring plasmid pRK2013 Ditta et al., 1980 E. coli DH5α pYTSK01K_0G7 DH5α harboring plasmid pYTSK01K_0G7 This study E. coli DH5α pVLT33-PA_rhlABC DH5α harboring plasmid pVLT33-PA_rhlABC Wittgens et al., 2017 E. coli DH5α pBNT Km DH5α harboring plasmid pBNTmcs(t)Km This study S. cerevisiae VL6-48 pYTSK10K_0G7_rhlAB VL6-48 harboring plasmid pYTSK10K_0G7_rhlAB This study E. coli DH5α pYTSK10K_1G7_rhlAB DH5α harboring plasmid pYTSK10K_1G7_rhlAB This study E. coli DH5α pRhon5Hi-2-eyfp DH5α harboring plasmid pRhon5Hi-2-eyfp Troost et al., 2019 S. cerevisiae VL6-48 pYTSK40K_1G7_rhlAB VL6-48 harboring plasmid pYTSK40K_1G7_rhlAB This study E. coli DH5α pYTSK40K_0G7_rhlAB DH5α harboring plasmid pYTSK40K_0G7_rhlAB This study E. coli S17-1 pYTSK40K_1G7_rhlAB S17-1 harboring plasmid pYTSK40K_1G7_rhlAB This study E. coli DH5αλpir pSK02 DH5α λpir harboring Tn7 delivery vector pSK02 for chromosomal integration Bator et al., 2020b E. coli DH5α pSW-2 DH5α harboring plasmid pSW-2 encoding I-SceI nuclease Martinez-Garcia and de Lorenzo, 2011 E. coli DH5αλpir pTNS-1 DH5α λpir harboring plasmid pTNS-1 Choi et al., 2005 E. coli DH5αλpir p pha DH5α λpir harboring plasmid p pha Mato Aguirre, 2019 E. coli PIR2 pEMG-flag1 PIR2 harboring plasmid pEMG-flag1 Blesken et al., 2020 E. coli PIR2 pEMG-flag2 PIR2 harboring plasmid pEMG-flag2 Blesken et al., 2020 E. coli DH5αλpir pMaW03 DH5α λpir harboring plasmid pMaW03 This study P. putida chassis strains P. putida KT2440 Wild type Bagdasarian et al., 1981 P. putida KT2440 flag PP_4328-PP_4344 and PP_4351-PP_4397 Blesken et al., 2020 P. putida KT2440 phaG PP_1408 This study P. putida KT2440 pha PP_5003-PP_5008 Blesken et al., 2020 P. putida KT2440 phaG pha PP_1408 and PP_5003-PP_5008 (phaC1ZC2DFI) This study P. putida KT2440 flag pha PP_4328-PP_4344, PP_4351-PP_4397 and PP_5003-PP_5008 This study P. putida strains used for biosurfactant production P. putida KT2440 pPS05 KT2440 harboring plasmid pPS05 Tiso et al., 2016 P. putida KT2440 SK4 rhlAB, attTn7 integrated, P 14ffg This study P. putida KT2440 sal mRL E SK40 rhlAB, eYFP, attTn7 integrated, P nagAa /nagR, Gm R , tnsABCD This study P. putida KT2440 flag SK4 PP_4328-PP_4344 and PP_4351-PP_4397, rhlAB, attTn7 integrated, P 14ffg Blesken et al., 2020 P. putida KT2440 phaG SK4 PP_1408, rhlAB, attTn7 integrated, P 14ffg This study P. putida KT2440 pha SK4 PP_5003-PP_5008, rhlAB, attTn7 integrated, P 14ffg Blesken et al., 2020 P. putida KT2440 phaG pha SK4 PP_1408 and PP_5003-PP_5008, rhlAB, attTn7 integrated, P 14ffg This study P. putida KT2440 flag pha SK4 PP_4328-PP_4344 and PP_4351-PP_4397 and PP_5003-PP_5008, rhlAB, attTn7 integrated, P 14ffg

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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