Extraction and Serological Properties of Mycobacterium Cell Surface and Excreted Proteins

Khaertynov, Kamil S.; Valeeva, Anna R.; Ivanov, Arkadiy V.; Mukminov, Malik N.; Urazov, Nail; Хаертынова, И. М.; Александрова, Н. М.; Moskvicheva, Albina V.; Ефимова, М. А.; Ахмадеев, Р. М.; Samigullina, Elvira S.; Nabatov, Alexey A.; Shuralev, Eduard A.

doi:10.1007/s12668-017-0492-1

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…It is abundant on the surface of MTB, so it can be used as a simulant of MTB. 29,30 We performed surface functionalization on the prepared sensor. First, the surface of the sensor was treated with oxygen plasma, and then the above packaging method was used to encapsulate the microfluidic chip biosensor.…”

Section: Preparation Of Biosensors and Integration Of Microfluidic Chipsmentioning

confidence: 99%

Rapid detection of airborne protein from Mycobacterium tuberculosis using a biosensor detection system

Jiang

et al. 2022

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Section: Preparation Of Biosensors and Integration Of Microfluidic Chipsmentioning

confidence: 99%

Rapid detection of airborne protein from Mycobacterium tuberculosis using a biosensor detection system

Jiang

et al. 2022

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“…The effectiveness of this approach was demonstrated earlier when developing diagnostic tests for other infections. [9] The question of increasing the declared properties of specific drugs can be solved using globulins obtained for diagnostically significant antigens of the rabies virus and polymethodic evaluation of their properties. [10,11] The purpose of this study was to optimize the conditions for the production of antirabic diagnostic immunoglobulins.…”

Section: Introductionmentioning

confidence: 99%

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Ефимова¹

2018

AJP

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Aim: The objective of this research was to obtain highly purified antirabic immunoglobulins to diagnostically significant fractions of the rabies virus antigen obtained by buoyant density separation in a step gradient of sucrose by ultracentrifugation at 30,000 g. Materials and Methods: As an immunogen, the rabies virus antigen of the "Ovechiy" GNKI strain with infectious titer Lg 10 −5.25 was used. Purification of the virus was carried out by separation in a stepwise gradient of sucrose 10-50% on Optima L-90K ultracentrifuge (Beckman) followed by gel filtration through the ENrich™ SEC 70 columns. Results: As a result of the separation, the fractions of the viral material, selected from the sucrose zone 10-20%, with the localization range of the antigenic determinants of 50-65 kDa were the most active, which was confirmed by the results of electrophoresis in the separating polyacrylamide gel and immunoblot. These characteristics of the obtained drug served as the basis for its use as a material for the hyperimmunization of clinically healthy sheep were carried out according to the generally accepted scheme. Hyperimmune serum with a titer of 1:6400-1:12800 in ELISA was used to isolate γ-globulin fractions by threefold reprecipitation with a saturated solution of ammonium sulfate, followed by concentration in dialysis bags, resulting in 5 final fractions of antirabic globulins. Conclusion: According to the results of ELISA and electrophoresis, globulin fractions were characterized by the greatest activity and specificity, the distribution of polypeptides in which corresponded to the location of antigenic determinants. The immunochemical properties of the obtained globulin drugs make them suitable in the future for use as specific components of improved ELISA-based express test systems for the diagnosis of rabies.

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An immunochromatographic test for the detection of anti-tuberculosis antibodies

Khammadov,

Khaertynov,

Akhmadeev

et al. 2023

jour

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Tuberculosis is a dangerous socially significant disease of various animal species. According to Rosselkhoznadzor data for 2022, the situation with bovine tuberculosis is endemic, stable, long-term trends are decreasing, epidemic thresholds for ill health and incidence have not been overcome. It should be noted that in 2022, bovine tuberculosis was not officially detected, but this infection was registered in several pigs and wild boars. Take a point that the reduction in the number of cases of tuberculosis in cattle is decreased (to zero), the overall strategy for combating this disease is successful, but this does not mean that the need to develop new tests for the accelerated diagnosis of this infection has completely disappeared. The safety of service personnel directly depends on minimizing the risk of infection with pathogens common to humans and animals, one of which is tuberculosis. The fastest way to indicate infectious agents is by immunochromatographic analysis. This publication is devoted to the details that it is desirable to focus on when developing a diagnostic test based on immunochromatographic analysis, using the example of bovine tuberculosis. As an antigen in the presented study, native antigens of Mycobacterium bovis grown on a nutrient medium Levenshtein-Jensen with proven antigenic activity were used. Conjugation of antispecies antibodies was carried out with a colloidal solution of gold, (diameter of particle is 25±0,9 nm). In the course of the work, the optimal conditions for the manufacture of a diagnostic test for the detection of anti-tuberculosis antibodies by immunochromatographic analysis were shown.

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Extraction and Serological Properties of Mycobacterium Cell Surface and Excreted Proteins

Cited by 3 publications

References 30 publications

Rapid detection of airborne protein from Mycobacterium tuberculosis using a biosensor detection system

Rapid detection of airborne protein from Mycobacterium tuberculosis using a biosensor detection system

Untitled

An immunochromatographic test for the detection of anti-tuberculosis antibodies

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