An immunochromatographic test for the detection of anti-tuberculosis antibodies

Abstract

Tuberculosis is a dangerous socially significant disease of various animal species. According to Rosselkhoznadzor data for 2022, the situation with bovine tuberculosis is endemic, stable, long-term trends are decreasing, epidemic thresholds for ill health and incidence have not been overcome. It should be noted that in 2022, bovine tuberculosis was not officially detected, but this infection was registered in several pigs and wild boars. Take a point that the reduction in the number of cases of tuberculosis in cattle is decreased (to zero), the overall strategy for combating this disease is successful, but this does not mean that the need to develop new tests for the accelerated diagnosis of this infection has completely disappeared. The safety of service personnel directly depends on minimizing the risk of infection with pathogens common to humans and animals, one of which is tuberculosis. The fastest way to indicate infectious agents is by immunochromatographic analysis. This publication is devoted to the details that it is desirable to focus on when developing a diagnostic test based on immunochromatographic analysis, using the example of bovine tuberculosis. As an antigen in the presented study, native antigens of Mycobacterium bovis grown on a nutrient medium Levenshtein-Jensen with proven antigenic activity were used. Conjugation of antispecies antibodies was carried out with a colloidal solution of gold, (diameter of particle is 25±0,9 nm). In the course of the work, the optimal conditions for the manufacture of a diagnostic test for the detection of anti-tuberculosis antibodies by immunochromatographic analysis were shown.