Extraction and quantification of phycobiliproteins from the red alga Furcellaria lumbricalis

Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

“…The PBPs are intracellular proteins that requires efficient cell disruption methods for high recovery yield. Numerous methods for PBP extraction from cyanobacteria and macroalgae have been described in the literature, and the choice of the suitable technique and efficiency of PBP extraction is dependent on the rigidity and chemical composition of the cell wall ( de Jesús et al, 2016 ; Tavanandi et al, 2018 ; Pan-utai and Iamtham, 2019 ; Saluri et al, 2019 ; Pereira et al, 2020 ). Researchers have reported the extraction efficiency of Na-phosphate buffer and the advantages of ultrasonic-assisted extraction technique compared with the conventional methods ( Hadiyanto and Suttrisnorhadi, 2016 ).…”

Red Seaweed Pigments from a Biotechnological Perspective

“…Common solvents applied and tested among researchers, to retrieve PE from red seaweeds, are phosphate buffer [35,46,50,58,69,70,73,78,79,89,90,93] and sodium phosphate buffer [53,103], featured prominently in Table 1. However, solvents such as potassium phosphate buffer [96], distilled water [45,77,83,91], acetic acid-sodium acetate [81,102], citrate buffer [57,74], diethyl ether [74], and EDTA [84], have also been found to be effective.…”

“…To separate PE from other pigments and compounds in red macroalgae, the pigment can be isolated and purified by either precipitation with ammonium sulfate [50], gel filtration chromatography [103], gel filtration and ion-exchange chromatography [69,72,73], expanded bed absorption and ion-exchange chromatography [80,83,94], centrifugal precipitation chromatography [85], size exclusion chromatography [57], preparative polyacrylamide gel electrophoresis (SDS-PAGE) [89,97,103], and hydroxyapatite chromatography (hydroxyapatite being a low-cost, laboratory-prepared chromatographic resin, which has also been used as an alternative to chromatography to purify PE from red seaweeds) [35,100,104]. A novel high-pressure liquid chromatography method, coupled with fluorescence and photodiode array detection, was developed recently by Saluri et al [57,74]; however, as seen by Table 1 for Furcellaria lumbricalis, studied by these authors, the results do not stand out when compared to results obtained by other authors; it is noteworthy, though (and already stated), that given the amount of variables pertaining to the method, species, or time of the year when harvesting, it is not unexpected to find a certain degree of variation in the results across the literature.…”

Biorefinery of marine macroalgae into high-tech bioproducts: a review

“…Phycobilins represent a group of pigments such as phycoerythrin, phycocyanin and allophycocyanin which are found in red algae and are commonly used as fluorescent reagents or as colourants in the food, dyes and cosmetic industries (Ragonese et al 2014;Mittal et al 2017). Phycobiliproteins are heat-sensitive pigment-protein complexes (Le Guillard et al 2016; Mittal et al 2017) with stable properties around 4-40 °C and pH values of 6-7 (Saluri et al 2019). Furthermore, these macroalgae biomolecules are known to have antioxidant, antidiabetic, anti-inflammatory and other medical properties (Ragonese et al 2014;Mittal et al 2017).…”