Extraction and characterisation of analytical grade C-phycocyanin from Euhalothece sp.

Mogany, Trisha; Kumari, Sheena; Swalaha, Feroz Mahomed; Bux, Faizal

doi:10.1007/s10811-018-1661-5

Cited by 32 publications

(17 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Common food preservatives, such as citric and benzoic acids, have also been shown to improve the thermostability of PC, which may be linked to a reduction in pH of the solution to levels that favour stability (e.g. pH 5.5) (Mishra et al, 2008;Mogany et al, 2019).…”

Section: Food Colourantsmentioning

confidence: 99%

Phycobiliproteins from extreme environments and their potential applications

Puzorjov

McCormick²

2020

Journal of Experimental Botany

View full text Add to dashboard Cite

The light-harvesting phycobilisome complex is an important component of photosynthesis in cyanobacteria and red algae. Phycobilisomes are composed of phycobiliproteins, including the blue phycobiliprotein phycocyanin, that are considered high-value products with applications in several industries. Remarkably, several cyanobacteria and red algal species retain the capacity to harvest light and photosynthesise under highly selective environments such as hot springs, and flourish in extremes of pH and elevated temperatures. These thermophilic organisms produce thermostable phycobiliproteins, which have superior qualities much needed for wider adoption of these natural pigment–proteins in the food, textile, and other industries. Here we review the available literature on the thermostability of phycobilisome components from thermophilic species and discuss how a better appreciation of phycobiliproteins from extreme environments will benefit our fundamental understanding of photosynthetic adaptation and could provide a sustainable resource for several industrial processes.

show abstract

Section: Food Colourantsmentioning

confidence: 99%

Phycobiliproteins from extreme environments and their potential applications

Puzorjov

McCormick²

2020

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…Ultrasonication produces cavitation effect, which, in turn, helps in the cell wall breakage allowing the solvent to penetrate into the biomass and increasing the contact surface area between the solvent and compounds, resulting in increased mass transfer of PC from cell into the solvent. In the subsequent freeze–thaw method, when cells are frozen, intracellular ice formation occurs, which causes further disruption of cell membrane during thawing ( Papadaki et al, 2017 ; Mogany et al, 2019 ). In a study with biomass of Arthospira platensis , higher PC extraction efficiency was attained with a combined cell disruption method of ultrasonication plus freezing and thawing ( Tavanandi et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

Khazi

Liaqat

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A620/A280 ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A620/A280 ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.

show abstract

“…(total phycobiliprotein (TPB): 128 ± 0.13 mg/g) [ 24 ], Oscillatoria quadripunctulata (PC: 137.15 mg/g) [ 34 ] and Euhalothece sp. (PC: 75 mg/g) [ 37 ]. On the other hand, freezing in liquid nitrogen and thawing at 4 °C yielded a lower amount and quality of PC [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of the Freezing-Thawing Method for Extracting Phycobiliproteins from Arthrospira sp.

et al. 2020

View full text Add to dashboard Cite

The freezing–thawing method had been reported to be the best phycobiliprotein extraction technique. However, optimum parameters of this extraction method for Arthrospira sp. (one of the major phycobiliprotein sources) still remained unclear. Hence, this study aimed to optimize the freezing–thawing parameters of phycobiliprotein extraction in Arthrospira sp. (UPMC-A0087). The optimization of the freezing–thawing method was conducted using different solvents, biomass/solvent ratios, temperatures, time intervals and freezing–thawing cycles. The extracted phycobiliproteins were quantified using a spectrophotometric assay. Double distilled water (pH 7) with a 0.50% w/v biomass/solvent ratio was the most efficient solvent in extracting high concentrations and purity of phycobiliproteins from Arthrospira sp. In addition, the combination of freezing at −80 °C (2 h) and thawing at 25 °C (24 h) appeared to be the optimum temperature and extraction time to obtain the highest amount of phycobiliproteins. A minimum of one cycle of freezing and thawing was sufficient for extracting high concentrations of phycobiliproteins. The findings from this study could reduce the cost and labor needed for extracting high quality phycobiliproteins. It also allowed the harvesting of large amounts of valuable phycobiliproteins.

show abstract

Extraction and characterisation of analytical grade C-phycocyanin from Euhalothece sp.

Cited by 32 publications

References 66 publications

Phycobiliproteins from extreme environments and their potential applications

Phycobiliproteins from extreme environments and their potential applications

Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

Optimization of the Freezing-Thawing Method for Extracting Phycobiliproteins from Arthrospira sp.

Contact Info

Product

Resources

About