Optimization of the Freezing-Thawing Method for Extracting Phycobiliproteins from Arthrospira sp.

Abstract

The freezing–thawing method had been reported to be the best phycobiliprotein extraction technique. However, optimum parameters of this extraction method for Arthrospira sp. (one of the major phycobiliprotein sources) still remained unclear. Hence, this study aimed to optimize the freezing–thawing parameters of phycobiliprotein extraction in Arthrospira sp. (UPMC-A0087). The optimization of the freezing–thawing method was conducted using different solvents, biomass/solvent ratios, temperatures, time intervals and freezing–thawing cycles. The extracted phycobiliproteins were quantified using a spectrophotometric assay. Double distilled water (pH 7) with a 0.50% w/v biomass/solvent ratio was the most efficient solvent in extracting high concentrations and purity of phycobiliproteins from Arthrospira sp. In addition, the combination of freezing at −80 °C (2 h) and thawing at 25 °C (24 h) appeared to be the optimum temperature and extraction time to obtain the highest amount of phycobiliproteins. A minimum of one cycle of freezing and thawing was sufficient for extracting high concentrations of phycobiliproteins. The findings from this study could reduce the cost and labor needed for extracting high quality phycobiliproteins. It also allowed the harvesting of large amounts of valuable phycobiliproteins.

The Critical Studies of Fucoxanthin Research Trends from 1928 to June 2021: A Bibliometric Review

“…In addition, other parameters such as solvent types, solvent volume, temperature, etc., are crucial in determining the concentration and purity of fucoxanthin. Therefore, optimum conditions of these parameters are necessary for obtaining the highest wield possible [140]. Several review articles have discussed in depth the extraction and purification of fucoxanthin.…”

A Recommendation for a Pre-Standardized Marine Microalgal Dry Weight Determination Protocol for Laboratory Scale Culture Using Ammonium Formate as a Washing Agent

“…Biomass determination is indispensable in microalgae studies as it is a crucial parameter in estimating biomass concentration, productivity, and percentages of particular cell components [ 10 , 11 ]. Yet, various settings of several parameters in microalgal DW determination have been utilized in previous studies ( Table 1 ), and accurate comparison is almost impossible.…”

“…Zhu and Lee [ 6 ] studied four different washing agents on marine microalgae and recommended either ammonium formate or ammonium bicarbonate to obtain accurate DW values. Two different approaches frequently utilized for microalgal biomass studies are DW [ 10 , 11 ] and ash-free dry weight (AFDW) [ 12 ]. Although AFDW is typically considered as the most accurate predictor for biomass determination [ 13 ], DW is often preferred because the process of obtaining AFDW is a time-consuming, expensive, and tedious procedure [ 14 ].…”

Uncovering Research Trends of Phycobiliproteins Using Bibliometric Approach

“…Hence, it is critical to focus on each step of the phycobiliproteins production process to improve the accumulation of high-quality phycobiliproteins from each species [1]. Several studies have been carried out in depth for this purpose [1,2,71,72]. For example, Manirafasha et al [5] reviewed the techniques to increase phycobiliprotein production, from algae strain selection to culture parameter optimization and phycobiliprotein extraction to phycobiliprotein purification.…”

“…The bioactivity properties of phycobiliproteins (specifically antioxidant activity) have piqued the interest of researchers since 2011 and have remained a research frontier until now [1,5]. Economically sustainable and environmentally friendly phycobiliprotein extraction methods have also gained popularity and have helped broaden the consumer acceptability of cheaper and safer natural pigments [71,72,97,98].…”