Extract from Conyza canadensis as a modulator of plasma protein oxidation induced by peroxynitrite in vitro

Abstract

Abstract:The antioxidative activity of the extract from Conyza canadensis in plasma treated with peroxynitrite (ONOO -) (0.1 mM) was studied. C. canadensis is known to possess a broad set of pharmacological effects because of content of various antioxidants, antiplatelet and anticoagulant compounds. The aim of our study was to assess if this extract protects plasma proteins against oxidative/nitrative damages induced by ONOO -. The plasma components are continuously exposed to reactive oxygen/nitrogen species action. Peroxynitrite evokes oxidative stress and induces undesirable effects in biological systems and causes damage to biomolecules. The extract from Conyza (50 -2500 mg/ml) caused a dose-dependent reduction of protein nitration by 90%. The oxidation of plasma proteins was diminished by about 75%. ONOO -oxidized the plasma thiol groups and this process was inhibited by tested extract. The level of reduced protein thiols was increased thrice at the lowest concentration of extract (50 mg/ml). The highest concentration of extract decreased twice the level of protein thiols in reduced forms and increased the homocysteine level about 4.5 times. The obtained results demonstrated that the extract from Conyza possesses antioxidative properties in vitro, protects plasma proteins against toxicity induced by peroxynitrite and has modulating effects on thiol/disulfide redox status.

Effectivity of polyphenolic polysaccharide-proteins isolated from medicinal plants as potential cellular immune response modulators

“…The antioxidant activity of Echinacea polysaccharides has been supported by study of Lee et al (2009). Next, the antioxidant effect of polyphenolic-polysaccharide conjugates from Erigeron and other selected medicinal plants was demonstrated by Saluk-Juszczak et al ( 2010a and 2010b ).…”

Medicinal plants possessed antioxidant and free radical scavenging effects (part 3)- A review

“…The highest concentration of extract decreased twice the level of protein thiols in reduced forms and increased the homocysteine level about 4.5 times. Accordingly, extract possessed antioxidative properties in vitro, protected plasma proteins against toxicity induced by peroxynitrite and had modulating effects on thiol/disulfide redox status [128].The protective effects of the polysaccharide extract from the plant on platelet proteins against nitrative and oxidative damage induced by ONOO − were studied . The extract of the plant distinctly reduced oxidation and nitration of proteins in blood platelets treated with ONOO − (0.1 mM) and O 2 production in these cells.…”

LC–DAD–MS Phenolic Characterisation of Six Invasive Plant Species in Croatia and Determination of Their Antimicrobial and Cytotoxic Activity

“…Previous phytochemical studies revealed the presence of different bioactive compounds in those species: Ailanthus altissima (Mill.) Swingle [ 16 ], Ambrosia artemisiifolia L. [ 17 ], Conyza canadensis (L.) Cronquist [ 18 ], Dittrichia viscosa (L.) Greuter [ 19 ], Xanthium strumarium L. [ 20 ], and Erigeron annuus (L.) Pers. [ 21 ].…”