Extracellular virion proteins of two Chinese CyHV-3/KHV isolates, and identification of two novel envelope proteins

Virus genomes and virus-host interactions in aquaculture animals

“…In recent years, after cyprinid herpesvirus disease became widely reported in the world, (especially in China) [145][146][147], numerous studies on cyprinid herpesviruses and identification of immune-related genes have been conducted [148][149][150]. It is envisaged, therefore, that new insight and better understanding of these cyprinid herpesviruses will emerge and lead to efficient antiviral approaches being developed in the near future.…”

Cyprinid Herpesvirus 3

“…More recently, two independent studies explored the structural proteome of one European and two Chinese CyHV-3 isolates by a combination of virus particle purification, gel electrophoresis, and mass spectrometry-based proteomic approaches (Michel, Leroy, et al, 2010;Yi et al, 2014). A total of 34 structural proteins were identified for all 3 CyHV-3 isolates, and another 12 proteins were found in only 1 or 2 of the 3 studied isolates (Table 6 and Fig.…”

“…Indeed, the architecture and protein composition of fish herpesvirus capsids generally mirror that of mammalian herpesviruses, with the exception of the small protein which forms the hexon tips in mammalian herpesviruses (Booy et al, 1996;Davison & Davison, 1995). Comparison with the closely related AngHV-1 resulted in the identification of 11 tegument or tegumentassociated proteins, including the large tegument protein ORF62 (Michel, Leroy, et al, 2010;van Beurden, Leroy, et al, 2011;Yi et al, 2014). Bioinformatical predictions for signal peptides, transmembrane domains, and glycosylation allowed the identification of a total of 16 putative membrane proteins (Aoki et al, 2007;Michel, Leroy, et al, 2010;Yi et al, 2014).…”

“…In addition, several studies dedicated to specific virion proteins have been carried out (Aoki et al, 2011;Dong et al, 2011;Fuchs, Granzow, Dauber, Fichtner, & Mettenleiter, 2014;Rosenkranz et al, 2008;Tu et al, 2014;Vrancken et al, 2013;Yi et al, 2014). Some of these proteins have been studied in more detail, notably ORF81, which is a type 3 membrane protein and is thought to be one of the most immunogenic (major) membrane van Beurden, Leroy, et al (2011).…”

“…Based on their high abundance and unique locations upon SDS-PAGE of purified proteins, Yi et al (2014) marked ORF43, ORF51, ORF62, ORF68, ORF72, ORF84, and ORF92 as the major structural proteins of CyHV-3 (Yi et al, 2014). Two of these proteins, namely the large tegument protein encoded by ORF62 and ORF68, had previously been identified as major antigenic CyHV-3 proteins by immunoscreening (Aoki et al, 2011).…”