Extracellular production of reactive oxygen species during seed germination and early seedling growth in Pisum sativum

Kranner, Ilse; Roach, Thomas; Beckett, Richard P.; Whitaker, C.; Minibayeva, Farida

doi:10.1016/j.jplph.2010.01.019

Cited by 130 publications

(100 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The signaling effect of galactinol and RFOs (Keunen et al 2013) seems to be related to the formation of reactive oxygen species (ROS) in tissues exposed to a broad range of abiotic stresses (Rosenwasser et al 2013). The production of extracellular ROS during pea seed germination and seedling development (Kranner et al 2010) is accelerated during seedling desiccation (Roach and Kranner 2011. It cannot be excluded that galactinol, raffinose and stachyose act as antioxidants Nishizawa-Yokoi et al 2008).…”

Section: Accumulation Of Galactinol and Rfosmentioning

confidence: 99%

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

et al. 2015

View full text Add to dashboard Cite

The objective of the present study was to recognize the molecular background of the accumulation of raffinose family oligosaccharides (RFOs) in pea (Pisum sativum L.) seedlings under osmotic stress conditions. The exposure of 5-day-old pea seedlings to osmotic stress for 48 h created by immersing roots in PEG8000 solution (-1.5 MPa) induced synthesis of galactinol and RFOs (raffinose and stachyose) in the epicotyl and root tissues, but not in cotyledons. After 24 h of recovery, galactinol completely disappeared, raffinose decreased fourfold and stachyose decreased twofold in roots, but increased in epicotyls. The temporary accumulation of RFOs resulted from a dramatic increase in the enzymatic activity and changes in expression of galactinol synthase (PsGolS), raffinose synthase (PsRS) and stachyose synthase (PsSTS) genes. PsGolS was induced by osmotic stress in both epicotyls as well as in roots. PsRS and PsSTS were induced only in epicotyls, but repressed or remained unaffected in roots, respectively. During recovery, the expression and activity of PsGolS, PsRS and PsSTS dramatically decreased. The expression of PsGolS gene, that level of mRNA transcript significantly decreased during recovery and whose promoter region was identified to contain some stress-related regulating elements, seems to play a crucial role in the biosynthesis of RFOs under osmotic stress. Possible signals that may trigger the induction of expression of PsGolS, PsRS and PsSTS genes and accumulation of RFOs in pea seedlings are discussed.

show abstract

Section: Accumulation Of Galactinol and Rfosmentioning

confidence: 99%

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Thus, it is possible to suppose action of the enzyme already in the initial periods of germination. Kranner et al (2010) observed an increase in O 2 -production with an increase in temperature during experiments carried out with Pisum sativum seeds. Wang et al (2012) observed an increase in production of ROSs, especially hydrogen peroxide and an increase in lipid peroxidation in soybean seeds under high temperature and moisture conditions.…”

Section: Resultsmentioning

confidence: 84%

Production of reactive oxygen species in Dalbergia nigra seeds under thermal stress

et al. 2014

View full text Add to dashboard Cite

-Seed germination is dependent on abiotic factors, temperature being one of the main ones, whose influence causes seed damage under extreme conditions. The aim of the present study was to investigate the effect of different temperatures during germination of D. nigra seeds and their physiological and biochemical implications. We assessed germination percentage and production of superoxide anion (O 2 -) and hydrogen peroxide (H 2 O 2 ) in seeds subjected to temperatures of 5, 15, 25, 35 and 45 ºC for different periods of time. Hydration is promoted at 45 ºC and inhibited at 5ºC, without germination in either, whereas it is minimal at 15 °C and at a maximum level at 25 °C. Superoxide production increases at higher temperatures (25 and 35 ºC) after 72 hours of hydration, coinciding with the beginning of radicle protrusion. Production of hydrogen peroxide decreases at all temperatures, except for 5 ºC, with values near each other at temperatures of 15, 25, and 35 ºC, where there was radicle protrusion. Termos para indexação: floresta, fisiologia, bioquímica, jacarandá-da-bahia.

show abstract

“…Alterations in extracellular redox potential might protect germinating seeds and young seedlings against pathogen attack (Schopfer et al 2001). Elevated amount of ROS in the surrounding medium of germinating embryos can also provide cell wall loosening to facilitate radical protrusion (Kranner et al 2010;Müller et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Gaseous molecules acting as inhibitors of mitochondrial respiration chain, mainly inhibitors of cytochrome c oxidase, such as hydrogen cyanide (HCN) or nitric oxide (NO) may alleviate seed dormancy and/or stimulate germination (Bailly et al 2008;Bethke et al 2006;Gniazdowska et al 2010a, b;Kranner et al 2010;Oracz et al 2009). Moreover, both HCN and NO trigger ROS production in germinating embryos of sunflower (Helianthus annuus) (Oracz et al 2009) and apple (Gniazdowska et al 2010b).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide and hydrogen cyanide as regulating factors of enzymatic antioxidant system in germinating apple embryos

Krasuska

Gniazdowska

2011

Acta Physiol Plant

View full text Add to dashboard Cite

Short-term (3 or 6 h) pre-treatment of apple (Malus domestica Borkh.) embryos with nitric oxide (NO) or hydrogen cyanide (HCN) induces transient accumulation of reactive oxygen species (ROS) leading to dormancy removal and germination. We demonstrated that enhanced NO emission by apple embryos during early phase of germination ''sensu stricto'' is required for seed transition from dormant into non-dormant state, and may be described by the model of ''nitrosative door'', analogous to ''oxidative window''. Cellular ROS concentration, resulting from NO or HCN embryo pre-treatment, seems to be under severe control of antioxidant system. Activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPX) and total peroxidases (Prxs) was determined during NO and HCNmediated germination ''sensu stricto'' of embryos. CAT and SOD activity increased transiently 24 h after embryos pre-treatment, while GR and Prx activity was stimulated mainly after 96 h. The most evident alterations were detected in GPX activity, being more than threefold stimulated by NO or HCN. Based on this results, we conclude that these reactive molecules act simultaneously crossing their signaling pathways and we propose that ROS, reactive nitrogen species, HCN at accurate level are essential during seed germination as signaling factors.

show abstract

Extracellular production of reactive oxygen species during seed germination and early seedling growth in Pisum sativum

Cited by 130 publications

References 30 publications

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

Osmotic stress induces genes, enzymes and accumulation of galactinol, raffinose and stachyose in seedlings of pea (Pisum sativum L.)

Production of reactive oxygen species in Dalbergia nigra seeds under thermal stress

Nitric oxide and hydrogen cyanide as regulating factors of enzymatic antioxidant system in germinating apple embryos

Contact Info

Product

Resources

About