Extracellular Matrix, Growth Factors, Genetics: Their Influence on Cell Proliferation and Myotube Formation in Primary Cultures of Adult Mouse Skeletal Muscle

Maley, Moira; Davies, Marilyn; Grounds, Miranda D.

doi:10.1006/excr.1995.1217

Cited by 82 publications

(66 citation statements)

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“…Reported effects of coating on MPC proliferation are controversial. Similar to our findings, mouse myoblasts at passage 0 had a proliferation rate that was independent of protein coating on tissue culture plastic (collagen type IV, laminin, and Matrigel) (20). In contrast, it has also been reported that proliferation was stimulated by different laminin isoforms (13,18,31,34) and collagen type IV (18) compared with BSA (31) or tissue culture plastic (18).…”

Section: Discussionsupporting

confidence: 89%

“…The BM is made up of networks of laminin and collagen type IV, linked by entactin, which provide attachment sites but also serve as a reservoir and regulator of growth factor supply to SCs. In vitro experiments have shown that several matrix proteins, including those present in the BM, affect myoblast proliferation and differentiation (13,18,20). Second, the elasticity of an in vitro environment (tissue culture plastic) is very different from the in vivo environment, which could also affect the stem cell phenotype.…”

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confidence: 99%

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Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation

Boonen¹,

Rosaria-Chak²,

Baaijens³

et al. 2009

American Journal of Physiology-Cell Physiology

115

108

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Boonen KJ, Rosaria-Chak KY, Baaijens FP, van der Schaft DW, Post MJ. Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation. Am J Physiol Cell Physiol 296: C1338 -C1345, 2009. First published March 25, 2009 doi:10.1152/ajpcell.00015.2009.-The use of muscle progenitor cells (MPCs) for regenerative medicine has been severely compromised by their decreased proliferative and differentiative capacity after being cultured in vitro. We hypothesized the loss of pivotal niche factors to be the cause. Therefore, we investigated the proliferative and differentiative response of passage 0 murine MPCs to varying substrate elasticities and protein coatings and found that proliferation was influenced only by elasticity, whereas differentiation was influenced by both elasticity and protein coating. A stiffness of 21 kPa optimally increased the proliferation of MPCs. Regarding differentiation, we demonstrated that fusion of MPCs into myotubes takes place regardless of elasticity. However, ongoing maturation with cross-striations and contractions occurred only on elasticities higher than 3 kPa. Furthermore, maturation was fastest on poly-D-lysine and laminin coatings.

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Section: Discussionsupporting

confidence: 89%

mentioning

confidence: 99%

Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation

Boonen¹,

Rosaria-Chak²,

Baaijens³

et al. 2009

American Journal of Physiology-Cell Physiology

115

108

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“…The SJL ր J and BALB ր c strains are compared because of the well-documented differences in their capacity for muscle regeneration (Mitchell et al 1992;Maley et al 1995;Roberts et al 1997). In addition, the pattern of muscle regeneration in normal mice is compared with that seen in TNF-␣ (-ր -) and TNF-␣ (-ր -) ր LT-␣ (-ր -) mice.…”

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confidence: 99%

The Role of Tumor Necrosis Factor-alpha (TNF-α) in Skeletal Muscle Regeneration

Collins

Grounds²

2001

J Histochem Cytochem.

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142

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The role of tumor necrosis factor-alpha (TNF-α), an important mediator of the inflammatory response after injury, was investigated in regenerating skeletal muscle. The pattern of expression of TNF-α during muscle regeneration was examined by immunohistochemistry in tissue sections of crush-injured or transplanted muscle autografts and in primary cultures of adult skeletal muscle. TNF-α was highly expressed in injured myofibers, inflammatory cells, endothelial cells, fibroblasts, and mast cells. Myoblasts and myotubes also expressed TNF-α in primary muscle cultures and tissue sections. The essential role of TNF-α and its homologue lymphotoxin-alpha (LT-α) during muscle regeneration was assessed by basic histology in TNF-α(–) and TNF-α(-/-)/LT-α(-/-) mice. No difference was apparent in the onset or pattern of muscle regeneration (i.e., inflammatory response, activation and fusion of myoblasts) between the two strains of null mice or between nulls and normal control mice. However, both strains of null mice appeared more prone to bystander damage of host muscle and regeneration distant from the site of injury/transplantation. Although expression of TNF-α may play an important role in muscle regeneration, the studies in the null mice show that redundancy within the cytokine system (or some other response) can effectively compensate for the absence of TNF-α in vivo. (J Histochem Cytochem 49:989–1001, 2001)

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“…Further, extracellular matrix composition (Matrigel, BD Biosciences for gels and the Greiner Bio-One coating for glass) may also change cell behavior. 16,36,37 Hence, different gel coating may affect the outcome. Yet, pair-wise comparison was always performed using matching matrix composition.…”

Section: Discussionmentioning

confidence: 99%

Intracellular tension in periosteum/perichondrium cells regulates long bone growth

Foolen

Donkelaar

Ito

2010

Journal Orthopaedic Research

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Perichondrium/periosteum is involved in regulating long bone growth. Long bones grow faster after removal or circumferential division of periosteum. This can be countered by culturing them in conditioned medium from perichondrium/periosteum cells. Because both complete removal and circumferential division are effective, we hypothesized that perichondrium/periosteum cells require an intact environment to release the appropriate soluble factors. More specifically, we propose that this release depends on their ability to generate intracellular tension. This hypothesis was explored by modulating the ability of perichondrium/periosteum cells to generate intracellular tension and monitoring the effect thereof on long bone growth. Perichondrium/periosteum cells were cultured on substrates with different stiffness. The medium produced by these cultures was added to embryonic chick tibiotarsi from which perichondrium/periosteum was either stripped or left intact. After 3 culture days, long bone growth was proportionally related to the stiffness of the substrate on which perichondrium/periosteum cells were grown while they produced conditioned medium. A second set of experiments demonstrated that the effect occurred through expression of a growth-inhibiting factor, rather than through the reduction of a stimulatory factor. Finally, evidence for the importance of intracellular tension was obtained by showing that the inhibitory effect was abolished when perichondrium/periosteum cells were treated with cytochalasin D, which disrupts the actin microfilaments. Thus, we concluded that modulation of long bone growth occurs through release of soluble inhibitors by perichondrium/periosteum cells, and that the ability of cells to develop intracellular tension through their actin microfilaments is at the base of this mechano-regulated control pathway.

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Extracellular Matrix, Growth Factors, Genetics: Their Influence on Cell Proliferation and Myotube Formation in Primary Cultures of Adult Mouse Skeletal Muscle

Cited by 82 publications

References 0 publications

Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation

Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation

The Role of Tumor Necrosis Factor-alpha (TNF-α) in Skeletal Muscle Regeneration

Intracellular tension in periosteum/perichondrium cells regulates long bone growth

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