Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation

Abstract

Boonen KJ, Rosaria-Chak KY, Baaijens FP, van der Schaft DW, Post MJ. Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation. Am J Physiol Cell Physiol 296: C1338 -C1345, 2009. First published March 25, 2009 doi:10.1152/ajpcell.00015.2009.-The use of muscle progenitor cells (MPCs) for regenerative medicine has been severely compromised by their decreased proliferative and differentiative capacity after being cultured in vitro. We hypothesized the loss of pivotal niche factors to be the cause. Therefore, we investigated the proliferative and differentiative response of passage 0 murine MPCs to varying substrate elasticities and protein coatings and found that proliferation was influenced only by elasticity, whereas differentiation was influenced by both elasticity and protein coating. A stiffness of 21 kPa optimally increased the proliferation of MPCs. Regarding differentiation, we demonstrated that fusion of MPCs into myotubes takes place regardless of elasticity. However, ongoing maturation with cross-striations and contractions occurred only on elasticities higher than 3 kPa. Furthermore, maturation was fastest on poly-D-lysine and laminin coatings.

Effect of Mesenchymal Cells on Myoblast Sheets Embedded in Collagen Gel

“…An adequate concentration of collagen IV is important to the microenvironment for satellite cells in terms of both regulation of muscle regeneration and satellite cell homeostasis 2,8,19,20) . In the present study, collagen expression differed between the Gel and OS conditions (Fig.…”

Intracellular tension in periosteum/perichondrium cells regulates long bone growth

“…Further, extracellular matrix composition (Matrigel, BD Biosciences for gels and the Greiner Bio-One coating for glass) may also change cell behavior. 16,36,37 Hence, different gel coating may affect the outcome. Yet, pair-wise comparison was always performed using matching matrix composition.…”

“…The relation between gel composition and stiffness was based on previous work. 16,17 These stiffnesses were chosen to span the microenvironment range from soft (brain tissue) to hard biological tissue (collagenous osteoid precursors of bone). 18 To polymerize the mixture of acrylamide (Fluka, St. Louis, MO) and bisacrylamide (Fluka), TEMED (Merck, Darmstadt, Germany) and ammonium persulfate (APS, Fisher Scientific, Breda, The Netherlands) were added with the appropriate amount of de-ionized water.…”

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

“…Laminin, entactin, fibronectin and tenascins are other proteins that constitute the ECM (Thorsteinsdottir et al, 2011). In vitro, various ECM components of the muscle tissue influence the muscle cell behavior (Boonen et al, 2009;Wilschut et al, 2010). Muscle cells grown on laminin-coated substrates resulted in more and thinner myotubes compared with myotubes on collagen type IV and matrigel coating (Boonen et al, 2009) and an improved myogenic differentiation (Wilschut et al, 2010).…”

“…In vitro, various ECM components of the muscle tissue influence the muscle cell behavior (Boonen et al, 2009;Wilschut et al, 2010). Muscle cells grown on laminin-coated substrates resulted in more and thinner myotubes compared with myotubes on collagen type IV and matrigel coating (Boonen et al, 2009) and an improved myogenic differentiation (Wilschut et al, 2010). Since these differences can be both species (Boonen et al, 2009), and cell type dependent (Macfelda et al, 2007), it is important to examine how ECM components influence in vitroin vitro bovine primary muscle cells.…”