Extracellular enzymatic profiles and taxonomic identification of endophytic fungi isolated from four plant species

Alberto, Raiani Nascimento; Costa, Alessandra Tenório; Polônio, Julio Cesar; Santos, Manoel Luíz dos; Rhoden, Sandro Augusto; Azevedo, João Lúcio de; Pamphile, João Alencar

doi:10.4238/gmr15049016

Cited by 15 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is the first report of hydrocarbon biodegradation by a fungus from the genus Saccharicola (97% ID). However, the production of extracellular protease enzymes has been confirmed in individuals of the genus [63], additional studies have identified fungal proteases due to their hydrolytic activity could participate in hydrocarbon degradation processes [57] [58].…”

Section: Discussionmentioning

confidence: 99%

Total Petroleum Hydrocarbon Degradation by Endophytic Fungi from the Ecuadorian Amazon

Marín¹,

Navarrete²,

Narváez-Trujillo³

2018

AiM

View full text Add to dashboard Cite

The capacity of 133 fungal endophyte isolates for degrading petroleum hydrocarbons was evaluated. The endophytes were isolated from leaf and stem tissues from 23 plants collected in a natural habitat contaminated with crude oil in southwestern Ecuador. Their capacity for hydrocarbon biodegradation was tested by an in vitro colorimetric qualitative test during 10 days, using the Minimal Salt Medium and crude oil as the carbon source. Taxonomic identification of the endophytic fungi that showed bioactivity in the qualitative test was carried out by analysis of the ITS gene of the region 18S of the rDNA. Endophytes showed the best results in the previous qualitative test where selected for a quantitative in vitro test for 30 days. Residual hydrocarbons were tracked by infrared spectroscopy (IR) and gas chromatography (GC) with a flame ionization detector. The maximum removal rates of total petroleum hydrocarbons were 99.6% (IR) and 99.8% (GC), corresponding to fungi of the genus Verticillium sp. and Xylaria sp. 1 respectively. This is the first report of biodegradation of crude oil hydrocarbons by endophytic fungi in a tropical ecosystem. The results suggest these fungal isolates are potential hydrocarbon biodegraders that could be used in bioremediation processes.

show abstract

Section: Discussionmentioning

confidence: 99%

Total Petroleum Hydrocarbon Degradation by Endophytic Fungi from the Ecuadorian Amazon

Marín¹,

Navarrete²,

Narváez-Trujillo³

2018

AiM

View full text Add to dashboard Cite

show abstract

“…In vitro cellulase and amylase activity was analyzed for 43 strains belonging to some of the most abundant taxa. The production of cellulase was assayed using the method described by Sunitha et al (2013) adapted to PDA plates. For each fungal strain a 6 mm diameter mycelial disk was placed in the center of a 9 cm.…”

Section: Methodsmentioning

confidence: 99%

A Survey of Culturable Fungal Endophytes From Festuca rubra subsp. pruinosa, a Grass From Marine Cliffs, Reveals a Core Microbiome

et al. 2019

View full text Add to dashboard Cite

Festuca rubra subsp. pruinosa is a perennial grass that inhabits sea cliffs of the Atlantic coasts of Europe. In this unhospitable environment plants grow in rock crevices and are exposed to abiotic stress factors such as low nutrient availability, wind, and salinity. Festuca rubra subsp. pruinosa is a host of the fungal endophyte Epichloë festucae, which colonizes aerial organs, but its root mycobiota is unknown. The culturable endophytic mycobiota of FRP roots was surveyed in a set of 105 plants sampled at five populations in marine cliffs from the northern coast of Spain. In total, 135 different fungal taxa were identified, 17 of them occurred in more than 10% of plants and in two or more populations. Seven taxa belonging to Fusarium, Diaporthe, Helotiales, Drechslera, Slopeiomyces, and Penicillium appeared to be constituents of the core microbiome of Festuca rubra subsp. pruinosa roots because they occurred in more than 20% of the plants analyzed, and at three or more populations. Most fungal strains analyzed (71.8%) were halotolerant. The presence of Epichloë festucae in aboveground tissue was detected in 65.7% of the plants, but its presence did not seem to significantly affect the structure of the core or other root microbiota, when compared to that of plants free of this endophyte. When plants of the grass Lolium perenne were inoculated with fungal strains obtained from Festuca rubra subsp. pruinosa roots, a Diaporthe strain significantly promoted leaf biomass production under normal and saline (200 mM NaCl) watering regimes. These results suggest that the core mycobiome of Festuca rubra subsp. pruinosa could have a role in host plant adaptation, and might be useful for the improvement of agricultural grasses.

show abstract

“…In the current study, more than 85% of the isolates produced all these three enzymes which is in agreement with the previous reports of endophytic fungi from two mangrove species (Raviraja, 2005). Notable enzyme production (amylase, lipase and protease) could be observed for endophytic fungal species from medicinal plants also (Alberto 2016, Toghueo, 2017.…”

Section: Enzymatic Activitymentioning

confidence: 99%

“…It can be used in several areas, including food processing, leather industry, detergents, pharmaceuticals and medical therapy (Alberto, 2016;Lobo, 2005;Martinho, 2019).…”

Section: Introductionmentioning

confidence: 99%

Diversity and bioactive profile of endophytic mycoflora in mangroves

Revathy

Mohan

Kesavan

et al. 2022

Preprint

View full text Add to dashboard Cite

The present study deals with the mangrove associated endophytic fungi and their bioactivity profile. The fungal endophytes were isolated from the leaves of three mangrove species (Aegiceras corniculatum, Lumnitzera racemosa and Ceriops tagal) and identified by morphological and molecular methods (Internal Transcribed Spacer and β-tubulin (Ben A) sequencing). The isolates mainly belonged to Ascomycota (95%) and the Basidiomycota comprised only 5%. They come under 5 classes, 11 orders, 16 genera and 25 species. Under Ascomycota, four classes, viz., Sordariomycetes (44.5%), Eurotiomycetes (32.46%), Dothideomycetes (17.28%) and Saccharomycetes (1.83%) comprising 10 orders were recorded. Under Basidiomycota a single class viz., Agaricomycetes (3.93%) comprising 1 order (Agaricales) was observed. Colletotrichum (30.2%) was the dominant genus followed by Aspergillus (17.8%), Penicillium (14.1%), Phyllosticta (9.1%) etc. Penicillium citrinum was the dominant fungus (22%) in A. corniculatum; Colletotrichum siamense (31%) in L. racemosa and Aspergillus sydowii (35%) in C. tagal (Fig. 3). Among the isolates, the Colonization frequency (CF%) was maximum for Colletotrichum siamense (18%) followed by Phyllosticta capitalensis (11.6%). Penicillium citrinum was found in all the three plant species. Isolates from Ceriops tagal were found to exhibit higher antibacterial as well as hydrolytic enzyme production potential. Aspergillus montevidensis EF 30, Cladosporium oxysporum EF 316, Colletotrichum siamense EF 272, Penicillium chrysogenum EF 362, Colletotrichum fruticola EF 22, Ascotricha chartarum EF 374, Aspergillus sydowii EF 239, Talaromyces purpurogenus EF 313, Meyerozyma caribbica EF 347 and Schizophyllum commune EF 309 were segregated as potential strains for hydrolytic enzyme production. A. aculeatus EF 10, T. purpurogenus EF 313, C. oxysporum EF 316, C. fruticola EF 74, P. capitalensis EF 187, A. sydowi EF 226 and P. chrysogenum EF 363 showed notable antimicrobial property. T purprogenus EF 313 endowed with pigment (red) production has potential for application in dyeing industry. During the current study, 25 different species of endophytic fungal species could be recovered from three host mangrove plants and some of them were having high bioactive potential for possible commercial applications with respect to bioremediation and as antimicrobials in medicine and aquaculture.

show abstract

Extracellular enzymatic profiles and taxonomic identification of endophytic fungi isolated from four plant species

Cited by 15 publications

References 33 publications

Total Petroleum Hydrocarbon Degradation by Endophytic Fungi from the Ecuadorian Amazon

Total Petroleum Hydrocarbon Degradation by Endophytic Fungi from the Ecuadorian Amazon

A Survey of Culturable Fungal Endophytes From Festuca rubra subsp. pruinosa, a Grass From Marine Cliffs, Reveals a Core Microbiome

Diversity and bioactive profile of endophytic mycoflora in mangroves

Contact Info

Product

Resources

About