Extracellular Electrophysiological Measurements of Cooperative Signals in Astrocytes Populations

Mestre, A.; Inácio, Pedro M. C.; Elamine, Youssef; Asgarifar, Sanaz; Lourenço, Ana Sofia; Cristiano, Maria Lurdes Santos; Aguiar, Paulo; Medeiros, Maria C. R.; Araújo, Inês M.; Ventura, J.; Gomes, Henrique L.

doi:10.3389/fncir.2017.00080

Cited by 13 publications

(10 citation statements)

References 30 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the measurement of bioelectrical signals generated by non-electrogenic cells has started to attract attention. Using gold-based electrodes, we previously reported the recording of these low frequency signals in astrocyte populations and in glioma cells [25][26][27][28], and others have also reported the recording of signals in prostate cancer cells [29] using similar electrodes. Other approaches have used organic-based transistors to stimulate astrocytes [30] and silver nanowires to record astrocyte signals [31].…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Ultra-low noise PEDOT:PSS electrodes on bacterial cellulose: A sensor to access bioelectrical signals in non-electrogenic cells

Inácio

Medeiros

Carvalho

et al. 2020

Organic Electronics

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 95%

“…6 (c) for both voltage and current detection modes. The comparative effect of detecting signals in voltage or in current has been addressed in our previous works [26].…”

Section: Small Signal Impedance Of the Sensing Electrodesmentioning

confidence: 99%

Ultra-low noise PEDOT:PSS electrodes on bacterial cellulose: A sensor to access bioelectrical signals in non-electrogenic cells

Inácio

Medeiros

Carvalho

et al. 2020

Organic Electronics

View full text Add to dashboard Cite

“…We think improvements brought from the flexible electronics may improve neuroscientist's knowledge of the brain, indeed, despite numerous findings about many computational principles of the cerebral cortex underlying network dynamic pattern, many aspects remain unclear. For example, the role of glial cells, which represents the majority of the brain cells, is still controversial losing the old neuroscientists' perspective of merely supporting elements to active structures for the control of synaptic activity with an extracellular autonomous activity [17].…”

Section: Introductionmentioning

confidence: 99%

The rise of flexible electronics in neuroscience, from materials selection to in vitro and in vivo applications

Maiolo

Polese

Convertino

2019

Advances in Physics: X

View full text Add to dashboard Cite

Neuroscience deals with one of the most complicate system we can study: the brain. The huge amount of connections among the cells and the different phenomena occurring at different scale give rise to a continuous flow of data that have to be collected, analyzed and interpreted. Neuroscientists try to interrogate this complexity to find basic principles underlying brain electrochemical signalling and human/animal behaviour to disclose the mechanisms that trigger neurodegenerative diseases and to understand how restoring damaged brain circuits. The main tool to perform these tasks is a neural interface, a system able to interact with brain tissue at different levels to provide a uni/bidirectional communication path. Recently, breakthroughs coming from various disciplines have been combined to enforce features and potentialities of neural interfaces. Among the different findings, flexible electronics is playing a pivotal role in revolutionizing neural interfaces. In this work, we review the most recent advances in the fabrication of neural interfaces based on flexible electronics. We define challenges and issues to be solved for the application of such platforms and we discuss the different parts of the system regarding improvements in materials selection and breakthrough in applications both for in vitro and in vivo tests.

show abstract

“…Despite this agreement, future work is required to correlate the magnitude and duration of calcium transients with OCa 2+ Ts. While previous studies have reported simultaneous electrical stimulation and calcium imaging(Mestre et al, 2017b) none have simultaneously recorded OCa 2+ Ts using MEAs to date.…”

mentioning

confidence: 99%

Electrically-evoked oscillating calcium transients in mono- and co-cultures of iPSC glia and sensory neurons

Lawson

LaVancher

DeAlmeida

et al. 2023

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Activated glia are known to exhibit either neuroprotective or neurodegenerative effects, depending on their phenotype, while participating in chronic pain regulation. Until recently, it has been believed that satellite glial cells and astrocytes are electrically slight and process stimuli only through intracellular calcium flux that triggers downstream signaling mechanisms. Though glia do not exhibit action potentials, they do express both voltage- and ligand-gated ion channels that facilitate measurable calcium transients, a measure of their own phenotypic excitability, and support and modulate sensory neuron excitability through ion buffering and secretion of excitatory or inhibitory neuropeptides (i.e., paracrine signaling). We recently developed a model of acute and chronic nociception using co-cultures of iPSC sensory neurons (SN) and spinal astrocytes on microelectrode arrays (MEAs). Until recently, only neuronal extracellular activity has been recorded using MEAs with a high signal-to-noise ratio and in a non-invasive manner. Unfortunately, this method has limited compatibility with simultaneous calcium transient imaging techniques, which is the most common method for monitoring the phenotypic activity of astrocytes. Moreover, both dye-based and genetically encoded calcium indicator imaging rely on calcium chelation, affecting the culture’s long-term physiology. Therefore, it would be ideal to allow continuous and simultaneous direct phenotypic monitoring of both SNs and astrocytes in a high-to-moderate throughput non-invasive manner and would significantly advance the field of electrophysiology. Here, we characterize astrocytic oscillating calcium transients (OCa2+Ts) in mono- and co-cultures of iPSC astrocytes as well as iPSC SN-astrocyte co-cultures on 48 well plate MEAs. We demonstrate that astrocytes exhibit OCa2+Ts in an electrical stimulus amplitude- and duration-dependent manner. We show that OCa2+Ts can be pharmacologically inhibited with the gap junction antagonist, carbenoxolone (100 μM). Most importantly, we demonstrate that both neurons and glia can be phenotypically characterized in real time, repeatedly, over the duration of the culture. In total, our findings suggest that calcium transients in glial populations may serve as a stand-alone or supplemental screening technique for identifying potential analgesics or compounds targeting other glia-mediated pathologies.

show abstract

Extracellular Electrophysiological Measurements of Cooperative Signals in Astrocytes Populations

Cited by 13 publications

References 30 publications

Ultra-low noise PEDOT:PSS electrodes on bacterial cellulose: A sensor to access bioelectrical signals in non-electrogenic cells

Ultra-low noise PEDOT:PSS electrodes on bacterial cellulose: A sensor to access bioelectrical signals in non-electrogenic cells

The rise of flexible electronics in neuroscience, from materials selection to in vitro and in vivo applications

Electrically-evoked oscillating calcium transients in mono- and co-cultures of iPSC glia and sensory neurons

Contact Info

Product

Resources

About