Extracellular chitinase production by <i>Trichoderma harzianum</i> in submerged fermentation

Sandhya, Chandran; Adapa, Leela Krishna; Nampoothiri, K. Madhavan; Binod, Parameswaran; Szakács, George; Pandey, Ashok

doi:10.1002/jobm.200310284

Cited by 86 publications

(58 citation statements)

References 33 publications

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“…The optimum temperature reflects a balance between the effect on the activity and the maximum yield of the enzyme protein. This was positively correlated on the chitinase production by Trichoderma harzianum in submerged fermentation [14,15].…”

Section: Effect Of Ph and Temperaturementioning

confidence: 86%

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Parani¹,

Shetty²,

Saha³

2011

Indian J Microbiol

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Serratia marcescens, strain SR 1 was isolated from the local soil of a cultivated farm and it was screened as potent strain for chitinase production. Maximum chitinase production (77.3 u Mh -1 100 -1 ) was observed after 96 h of incubation period with pH 5.5 at 30°C under shake conditions (120 rpm). Compare to still flasks, shake culture with prawn fish colloidal chitin of 0.5% (w/v) concentration, showed a better enzyme yield. Crude enzyme showed antifungal activity against plant pathogens.

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Section: Effect Of Ph and Temperaturementioning

confidence: 86%

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Parani¹,

Shetty²,

Saha³

2011

Indian J Microbiol

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“…Production of commercially important enzymes such as amylases, cellulases, 1-3 beta glucanases, and chitinases were extensively studied and this technology is continuously being updated (Harman et al, 2004;Ahamed and Vermette, 2008;Sandhya et al 2004). Recently, they have been found useful in the production of silver nanoparticles (Maliszewska et al, 2009;Vahabi et al, 2011).…”

Section: Potential Applications In Modern Agriculture and Sustainablementioning

confidence: 99%

Trichoderma: A significant fungus for agriculture and environment

Waghunde¹,

Shelake²,

Sabalpara³

2016

Afr. J. Agric. Res.

128

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The novel technologies in all areas of agriculture have improved agricultural production, but some modern practices affect the environment. The recent challenge faced by advanced farming is to achieve higher yields in environment-friendly manner. Thus, there is an immediate need to find eco-friendly solutions such as wider application of biocontrol agents. Among various types of species being used as biocontrol agents, including fungi and bacteria, fungal genus Trichoderma produces different kinds of enzymes which play a major role in biocontrol activity like degradation of cell wall, tolerance to biotic or abiotic stresses, hyphal growth etc. The understanding of filamentous fungi belonging to the genus Trichoderma has continuously evolved since last two decades, from the simple concepts of biocontrol agents to their recently established role as symbionts with different beneficial effects to the plants. Recent findings from structural and functional genomics approaches suggest the additional use of these microbes as model to study mechanisms involved in multiple player interactions that is, microbes-microbes-plant-environment. In this work, historical development of Trichoderma spp., mode of action against different biological agents, potential applications and recent mass production techniques are summarized and discussed in detail with updated advances with their application in the agriculture and sustainable environment.

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“…The presence of easily available carbon source (dextrose), suppresses the chi-tinase production at first but in the later phases of growth there is little enhancement in chitinase production when the dextrose is fully or partially being used up. Sandhya et al (2004) documented the same pattern of chitinase production with the use of different carbon and nitrogen source for the fungus Trichoderma harzianum. Maximum chitinase activity was observed in Medium III constituting colloidal chitin as carbon source and Yeast extract as a nitrogen source, compared to the other three media.…”

Section: Discussionmentioning

confidence: 78%

Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme fromMetarhizium anisopliae isolates

Dhar

Kaur

2009

Ann. Microbiol.

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-Metarhizium anisopliae, an entomopathogenic hyphomycete, is being used effectively in Integrated Pest Management (IPM) system. Foliar application of these fungi is quite satisfactory as it invades its host by adhering to insect cuticles and formation of penetration structures called appresoria, which produces various extracellular enzymes, including chitinase that causes the insect cuticle breaching. The induction and repression mechanism of chitinase activity is not entirely understood and activity of this enzyme is different in response to different carbon and nitrogen sources. This report illustrates the effect of two carbon sources viz. colloidal chitin and dextrose and a nitrogen source, yeast extract on the chitinase production of fourteen M. anisopliae isolates. The chitinase activity varied among the isolates and the different media used. A high enzymatic activity was observed in the medium containing an extra nitrogen source (yeast extract) followed by the medium containing colloidal chitin as a sole source of carbon and nitrogen. The exochitinase activity and the chitinase activity gel were also studied for the isolates showing high chitinase enzyme production. An array of chitinase isozymes were observed on chitinase activity gel with a common 14.3 kDa enzyme for all the isolates.

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Extracellular chitinase production by Trichoderma harzianum in submerged fermentation

Cited by 86 publications

References 33 publications

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Trichoderma: A significant fungus for agriculture and environment

Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme fromMetarhizium anisopliae isolates

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