Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme fromMetarhizium anisopliae isolates

Dhar, Priyanka; Kaur, Gurvinder

doi:10.1007/bf03175144

Cited by 10 publications

(3 citation statements)

References 31 publications

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“…The optimum pH value here was found at 5 and the enzyme was adequately stable till pH 7. Same results were obtained for Aspergillus terrus, Beauveria bassiana and Metarhizium anisopliae [29,38,39].…”

Section: Discussionsupporting

confidence: 75%

Submerged production, partial purification and characterization of extracellular chitinase from local endophytic fungus for Culex pipiens biocontrol: Strategy for protein stabilization

et al. 2022

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The major barrier which restricts the enzymatic industrial application is their insufficient stability during processing. In this study, a native strain Aspergillus niger M-8, identified and deposited in GenBank under accession number MW940924, showed high chitinase production via submerged fermentation. The optimized operational conditions increased the enzyme production about 13 fold (up to 572.0 U/mg) using medium containing 0.1% untreated chitin, 0.1% starch and 0.3% tryptone at 35°C and pH 5. To increase the enzyme purification efficiency and stability, two co-solvent protein stabilizers (tryptone and yeast extract) were applied in the production medium and fractionation buffers. The results revealed that medium constituents, stabilizer type and pH greatly affected the yield and stability. Tryptone-optimized medium potentiated the enzyme specific activity (8885.0 U/mg), purification fold (16.3) and recovery (10.2%) more than the same medium supplemented with yeast extract (0.4%), under the same fractionation conditions. Furthermore, the fractionation buffer (acetate buffer, pH 5) containing 2 mM tryptone as stabilizer resulted in double the recovery (18.8%) in comparison to the yeast-containing buffer. The purified chitinase fraction using 40% (v/v) ethanol (F40) reached its optimum catalytic activity at a temperature of 30°C and pH 5. Yet, it was also stable and retained most of its initial activity at a wide range of temperature (25-70°C) and pH values (4-7). Interestingly, the more stabilized F40 chitinase had more virulence effect against mosquito larvae (LC95= 222.9 ppm) after 48 h, suggesting that it can be applied for biological control of Culex pipiens.

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Section: Discussionsupporting

confidence: 75%

Submerged production, partial purification and characterization of extracellular chitinase from local endophytic fungus for Culex pipiens biocontrol: Strategy for protein stabilization

et al. 2022

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“…Peptone and yeast extract as nitrogen sources proved to be positive regulators of chitinase (Lopes et al 2008). Dhar and Kaur (2009) reported high yields of chitinase from Metarhizium anisopliae in culture medium supplemented with yeast extract.…”

Section: Resultsmentioning

confidence: 96%

Partial purification and antifungal profile of chitinase produced by Streptomyces tendae TK-VL_333

Kavitha

Vijayalakshmi

2010

Ann Microbiol

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Production of chitinase by Streptomyces tendae strain TK-VL_333 isolated from laterite soils of the Guntur region, India, was studied in chitin yeast extract salts (CYS) broth. The effect of cultural and nutritional conditions on chitinase productivity of the strain was recorded. The strain elaborated high yields of chitinase when cultured in 1% w/v chitin-supplemented CYS broth containing 0.5% (w/v) xylose and 0.25% (w/v) yeast extract with an initial pH 7 and incubated at temperature 30°C for 72 h. In addition, this strain could utilize fungal biomass as a chitin supplement for chitinase production. The molecular weight of the enzyme purified by ammonium sulphate precipitation (80%) and Sephadex G-100 gel filtration was determined as 45 kDa by SDS-PAGE analysis. The partially purified enzyme was tested for colloidal chitin hydrolysis as well as antifungal activity. It showed a clear zone of colloidal chitin hydrolysis on chitin agar plates, and antifungal activity against Aspergillus niger and Fusarium oxysporum. This is the first report of the optimization and partial purification of chitinase produced by a terrestrial S. tendae.

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“…Comparably, Ferreira et al ( 2021 ) showed that culture medium supplemented with sodium nitrate and riboflavin enhanced protease activity and conidia production of Metarhizium robertsii . Similarly, additives such as soybean protein combined with wheat bran induced the protease activity of M. anisopliae (Kim et al 2020 ), and the supplementation with yeast extract improved the chitinase composition of M. anisopliae (Dhar and Kaur 2009 ). Also, it was demonstrated that C:N ratio is a crucial variable that induces enzyme expression, e.g.…”

Section: Resultsmentioning

confidence: 99%

Performance of Metarhizium rileyi Nm017: nutritional supplementation to improve production and quality conidia

Mejía,

Rocha,

Sanabria

et al. 2024

3 Biotech

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This study aimed to analyze the effect of nutritional supplements on improving conidia production of Metarhizium rileyi Nm017 at laboratory scale (yields of conidia/substrate and biomass/substrate, and substrate consumption). Also, the influence on quality parameters were evaluated (germination at 36 and 48 h, enzymatic activity, and insecticidal activity on Helicoverpa zea). Six treatments (T1–T6) were assessed and all of them reached maximum conidia concentration after 7 days fermentation, a feasible production timetable. Yields from treatment T6 (yeast extract + V8 juice) were 1.5–threefold higher than the other treatments. Conidia from T6 reached germinations of 56% and 12% at 36 and 48 h, respectively, higher than T1 (without supplements), which had the lowest values found. M. rileyi conidia obtained from treatment T6 had the highest enzymatic activity (0.45 U chitinase g−1, 0.28 U lipase g−1, and 1.29 U protease g−1). However, treatments with the highest conidia yields and enzymatic activity were not positively correlated to the efficacy against H. zea. When M. rileyi was produced on T5 (yeast hydrolysate + V8 juice), conidia were 35% more virulent than treatment T6. The findings evidenced the noticeable impact of nutritional substrate amended for conidia production and quality. This work showed the relevance of insecticidal activity assessment as a selection criterion in the mass production development of a biocontrol agent.

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Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme fromMetarhizium anisopliae isolates

Cited by 10 publications

References 31 publications

Submerged production, partial purification and characterization of extracellular chitinase from local endophytic fungus for Culex pipiens biocontrol: Strategy for protein stabilization

Submerged production, partial purification and characterization of extracellular chitinase from local endophytic fungus for Culex pipiens biocontrol: Strategy for protein stabilization

Partial purification and antifungal profile of chitinase produced by Streptomyces tendae TK-VL_333

Performance of Metarhizium rileyi Nm017: nutritional supplementation to improve production and quality conidia

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