Partial purification and antifungal profile of chitinase produced by Streptomyces tendae TK-VL_333

Abstract

Production of chitinase by Streptomyces tendae strain TK-VL_333 isolated from laterite soils of the Guntur region, India, was studied in chitin yeast extract salts (CYS) broth. The effect of cultural and nutritional conditions on chitinase productivity of the strain was recorded. The strain elaborated high yields of chitinase when cultured in 1% w/v chitin-supplemented CYS broth containing 0.5% (w/v) xylose and 0.25% (w/v) yeast extract with an initial pH 7 and incubated at temperature 30°C for 72 h. In addition, this strain could utilize fungal biomass as a chitin supplement for chitinase production. The molecular weight of the enzyme purified by ammonium sulphate precipitation (80%) and Sephadex G-100 gel filtration was determined as 45 kDa by SDS-PAGE analysis. The partially purified enzyme was tested for colloidal chitin hydrolysis as well as antifungal activity. It showed a clear zone of colloidal chitin hydrolysis on chitin agar plates, and antifungal activity against Aspergillus niger and Fusarium oxysporum. This is the first report of the optimization and partial purification of chitinase produced by a terrestrial S. tendae.

Chitinolytic Microorganisms and Their Possible Application in Environmental Protection

“…NRG4 Fusarium moniliforme , Aspergillus niger , Mucor rouxi , Rhizopus nigricans [17] Alcaligenes xylosoxydans Fusarium sp. Rhizoctonia bataticola [113] Stenotrohomonas maltophila Fusarium solani , F. oxysporum , R. solani , A. alternata [46, 100] Rhizobium sp Aspergillus flavus , Aspergillus niger , Curvularia lunata , F. oxysporum , Fusarium udum [97] Serratia marcescens strain B2 Botrytis cinerea [95] Vibrio pacini Mucor racemosus , Trichoderma viride , Zygorhynchus heterognmus , Candida albicans [3] Streptomyces halstedii AJ-7 Alternaria alternata , B. cinerea , S. lycopersici , F. oxysporum [49] Streptomyces hygroscopicus Colletotrichum gloeosporioides , Sclerotium rolfsii [80] Streptomyces tendae TK-VL_333 Aspergillus niger , F. oxysporum [51] Streptomyces sp. DA11 Aspergillus niger , Candida albicans [37] Streptomyces roseolus DH Aspergillus spp., Rhizopus chinensis , Penicillium spp., Mucor spp.[47] Streptomyces sporovirgulis Alternaria alternata[104] Streptomyces rimosus Alternaria alternata , F. solani [105]…”

“…Microbial CHIs weigh from 20 to 120 kDa, with most 20–60 kDa [ 49 , 51 ]. The optimum pH and temperature of CHIs are 5–8 and ~40 °C, respectively.…”

Utilization of palm oil mill effluent as a novel substrate for the production of antifungal compounds by Streptomyces philanthi RM-1-138 and evaluation of its efficacy in suppression of three strains of oil palm pathogen

“…The high antifungal activity of S. philanthi RM-1-138 was recorded at pH 7.0 that the maximum production of antifungal compounds from Streptomyces sp. was at pH 7.0 (Kavitha & Vijayalakshmi, 2011;Tanabe et al, 2000;Yano et al, 2008).…”

Response of the wood-decay fungus Schizophyllum commune to co-occurring microorganisms

“…In interactions with bacteria, antagonistic effects known for biocontrol organisms or antibiotics producers were verified [14,[58][59][60]. However, in the interactions with S. marcescens (producing the antifungal red pigment prodigiosin) and Pantoea agglomerans (known to produce the peptide antibiotic 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine; [61,62]), no growth retardation, but an increased growth of S. commune was observed, which seems noteworthy.…”