External quality control in the andrology laboratory: an experimental multicenter trial

Neuwinger, J.; Behre, Hermann M.; Nieschlag, Eberhard

doi:10.1016/s0015-0282(16)53709-1

Cited by 170 publications

(11 citation statements)

References 8 publications

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“…Since this method does not count sperm cells individually, a variation in each number on the scale was adapted to include concentrations that were between the values of the function. This is because even the objective method (Neubauer chamber) has a tolerance of 10% for each side, and there are reports of even greater variations (Brazil et al, 2004, Neuwinger et al, 1990. Tables 1 and 2 show the mean sperm concentration values (10 6 /mL) obtained by performing linear regression, as well as the lower and upper limits, for each degree of turbidity using each spermdensimeter.…”

Section: Resultsmentioning

confidence: 99%

A New Spermdensimeter to Determine the Sperm Concentration of Equine Semen

Lima

Freitag

Kozicki

2020

AVS

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This study aimed to develop a spermdensimeter for evaluating stallion semen in a practical and efficient manner. This method is an alternative to the standard method that utilizes a Neubauer chamber under field conditions, which is typically employed because the Karras spermdensimeter ® model (for bovine and swine) is not suitable for determining equine semen concentrations. Two spermdensimeter models were developed. The appliances were made out of acrylic material, and each one had different angulations, with 8° for Model A and 10° for Model B. We tested 123 semen samples from stallions to evaluate and calibrate the two spermdensimeter prototypes based on the Neubauer chamber. Samples were analyzed by two evaluators. These results were compared to those obtained using the Neubauer chamber. Linear regression was used to establish the scale of concentrations for each model. Additionally, the correlation between the scales and the values found using the Neubauer chamber was calculated. Linear regression demonstrated a precise adjustment for the concentration curves, resulting in r² = 0.9395 for Model A and r² = 0.9418 for Model B. The correlations for the concentrations were significant (p < 0.0001). They were high and negatively correlated, measuring-0.83 for Model A and-0.8 for Model B. We concluded that the spermdensimeters we developed were effective for evaluating stallion sperm concentrations. These models can be used in routine practices associated with equine reproduction, achieving the main purpose of this work.

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Section: Resultsmentioning

confidence: 99%

A New Spermdensimeter to Determine the Sperm Concentration of Equine Semen

Lima

Freitag

Kozicki

2020

AVS

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“…It is essential that the laboratory performs the analysis according to the current WHO guidelines and participate at an external quality control (EQC) program. In fact, wide discrepancies in the assessments of sperm count, motility, and morphology can result when comparing semen analysis outcomes from different laboratories [4,5]. Together with EQC programs, participating in periodical teaching courses improves the experience of the laboratory and allows to maximize the probability of obtaining the established standards of quality [6].…”

Section: Semen Analysis: General Conceptsmentioning

confidence: 99%

Clinical Interpretation of Semen Analysis

Krausz

Farnetani

2022

Practical Clinical Andrology

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Semen analysis is a fundamental step in the evaluation of the male fertility potential. However, fertility is a “couple-concept” implying the importance of the partner’s fertility status. In fact, a part from extreme conditions (e.g., azoospermia, necrozoospermia, total immotile spermatozoa, etc.) sperm parameters are not fully predictive for natural pregnancy. Semen analysis is divided into macroscopic and microscopic evaluation, and it provides information on the efficiency of spermatogenesis and the integrity of post-testicular structures. Based on the WHO reference values, it is possible to identify alterations of semen parameters, which may derive from many different etiologies. It is essential that the laboratory performs the analysis according to the current WHO guidelines and participate at an external quality control (EQC) program. The clinical interpretation of these alterations is the focus of this chapter.

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“…S emen analysis is one of the first diagnostic methods used to evaluate male infertility. While manual microscopic semen analysis by qualified staff remains the reference technique, significant intra-and inter-laboratory variability can occur [1]. Spermatozoa motility, morphology, and concentration can also be analyzed using automated methods.…”

Section: Technical Reportmentioning

confidence: 99%

Impact of the presence of debris on the semen analysis using an automated system (SQA-V)

Ilardo¹

2021

Int J Med Biochem

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Objectives: This study examined the influence of the manual assessment of debris on the results of sperm concentration, progressive motility, and normal morphology measured using the SQA-V system (Medical Electronic Systems LLC, Los Angeles, CA, USA). Methods: Sixty samples were analyzed simultaneously and independently by different operators using the manual technique and the SQA-V analyzer. Three measurements (sperm concentration, progressive motility, and normal morphology) of each sample were assessed using the 4 debris assessment levels: none/few, moderate, many, and gross. Results: Given an optimal assessment of debris, the study data indicate that the SQA-V provided results that had very good agreement with those of the manual method: sperm concentration (rho=0.987, regression analysis formula: y=0.961x+1.962, p<0.0001), progressive motility (rho=0.949, regression analysis formula: y=0.989x+0.418, p<0.0001), and normal morphology (rho=0.694, regression analysis formula: y=0.678x+4.061, p<0.0001). Underestimation of debris increased sperm concentration and decreased motility and normal morphology, while overestimation of debris decreased sperm concentration and increased motility and normal morphology. Conclusion:The results indicated that the performance of the SQA-V remains subject to the competency of the operator.

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External quality control in the andrology laboratory: an experimental multicenter trial

Cited by 170 publications

References 8 publications

A New Spermdensimeter to Determine the Sperm Concentration of Equine Semen

A New Spermdensimeter to Determine the Sperm Concentration of Equine Semen

Clinical Interpretation of Semen Analysis

Impact of the presence of debris on the semen analysis using an automated system (SQA-V)

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