A New Spermdensimeter to Determine the Sperm Concentration of Equine Semen

Abstract

This study aimed to develop a spermdensimeter for evaluating stallion semen in a practical and efficient manner. This method is an alternative to the standard method that utilizes a Neubauer chamber under field conditions, which is typically employed because the Karras spermdensimeter ® model (for bovine and swine) is not suitable for determining equine semen concentrations. Two spermdensimeter models were developed. The appliances were made out of acrylic material, and each one had different angulations, with 8° for Model A and 10° for Model B. We tested 123 semen samples from stallions to evaluate and calibrate the two spermdensimeter prototypes based on the Neubauer chamber. Samples were analyzed by two evaluators. These results were compared to those obtained using the Neubauer chamber. Linear regression was used to establish the scale of concentrations for each model. Additionally, the correlation between the scales and the values found using the Neubauer chamber was calculated. Linear regression demonstrated a precise adjustment for the concentration curves, resulting in r² = 0.9395 for Model A and r² = 0.9418 for Model B. The correlations for the concentrations were significant (p < 0.0001). They were high and negatively correlated, measuring-0.83 for Model A and-0.8 for Model B. We concluded that the spermdensimeters we developed were effective for evaluating stallion sperm concentrations. These models can be used in routine practices associated with equine reproduction, achieving the main purpose of this work.