Extensive multiplex PCR diagnostics reveal new insights into the epidemiology of viral respiratory infections

Nickbakhsh, Sema; Thorburn, Fiona; Wissmann, Beatrix von; McMenamin, Jim; Gunson, Rory; Murcia, Pablo R.

doi:10.1017/s0950268816000339

Cited by 61 publications

(85 citation statements)

References 37 publications

(49 reference statements)

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“…Rhinovirus infection was seen throughout the year with many of the other viruses only observed during the colder months in keeping with seasonal trends in the UK 25,28 . infection.…”

Section: Discussionsupporting

confidence: 60%

Outcomes of Nosocomial Viral Respiratory Infections in High-Risk Neonates

et al. 2016

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk Financial disclosure: The other authors have no financial relationships relevant to this article to disclose. Outcomes of nosocomial viral respiratory infections in high-risk neonates Conflicts of interest:The other authors have no potential conflicts of interest to disclose. Abbreviations:VRTIs -viral respiratory tract infections BPD -bronchopulmonary dysplasia NICU -neonatal intensive care unit NEC -necrotising enterocolitis CPAP -continuous positive airway pressure Bi-PAP -bi-level positive airway pressure HFOV -high frequency ventilation ECMO -extracorporeal membrane oxygenation RT-PCR -real time polymerase chain reaction RVP -respiratory viral panel NHS -National Health Service What's Known on This SubjectViral respiratory tract infections cause severe respiratory morbidity in ex-preterm infants after NICU discharge. They are now recognized to be more prevalent in the NICU but their longterm impact, prior to discharge, during this early period of life is unclear. What This Study AddsThis study identifies the adverse impact viral respiratory infections, particularly rhinovirus, have on newborn infants during their initial NICU admission. Identification of the associated significant respiratory morbidity and healthcare costs should focus efforts on reducing these nosocomially acquired infections. Contributors' Statements:Dr Don Sharkey conceptualized and designed the study, drafted the initial manuscript, and approved the final manuscript as submitted.Drs Shairbanu Zinna, Arthi Lakshmanan, Shin Tan, Shiu Soo, Lisa Szatkowski and Miss Rebecca McClaughry and Mr Martin Clarkson carried out the data collection, initial analyses, reviewed and revised the manuscript, and approved the final manuscript as submitted.All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work. ABSTRACTBackground Neonatal respiratory disease, particularly bronchopulmonary dysplasia, remains one of the leading causes of morbidity and mortality in newborn infants. There is increasing evidence that nosocomially acquired viral respiratory tract infections (VRTIs) are not uncommon in the neonatal intensive care unit but there are few studies on their impact on neonatal respiratory outcomes and the associated healthcare costs. This study aimed to assess the association between nosocomial VRTI and neonatal respiratory disease.

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“…Rhinovirus infection was seen throughout the year with many of the other viruses only observed during the colder months in keeping with seasonal trends in the UK 25,28 . infection.…”

Section: Discussionsupporting

confidence: 60%

Outcomes of Nosocomial Viral Respiratory Infections in High-Risk Neonates

et al. 2016

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“…Similar to the previous study, RV detected throughout the year with a peak in September. A Korean study in which the majority of the patients were children also reported RV as the number one etiologic factor …”

Section: Discussionmentioning

confidence: 99%

Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients

Appak

Duman

Belet

et al. 2019

Journal of Medical Virology

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Syndromic diagnosis by multiplex nucleic acid amplification tests is the most practical approach to respiratory tract infections since the symptoms are rarely agent‐specific. The aim of this study was to investigate the respiratory viruses in children admitted to a university hospital with acute respiratory tract infection during the last 8 years by a multiplex polymerase chain reaction (PCR) assay. A total of 3162 respiratory samples collected from children between April 2011 and April 2018 tested by a multiplex real‐time PCR assay. Two different commercial assays were used during the study period, "AusDiagnostics/Respiratory Pathogens 12 (AusDiagnostics)" used between April 2011 and December 2015, which changed to "Fast Track Diagnostics/Respiratory Pathogens 21 (Fast Track Diagnostics)" after January 2016 to cover more viruses. Nucleic acid extraction was done by EZ1 Advanced XL platform (QIAGEN). Respiratory pathogens detected in 1857 of the 3162 (58.7%) samples. The most prevalent viruses during the 8‐year period were rhinovirus/enterovirus (RV/EV; 36.2%), respiratory syncytial virus (RSV; 19%), and influenza virus A/B (14.7%). Rhinovirus was the main contributor to the RV/EV group as shown by the assay used during the 2016‐2018 period. RV/EV and adenoviruses detected throughout the year. Influenza virus was most frequently detected during January to March when both RSV and metapneumovirus were also in circulation. The coinfection percentage was 10.2%. Rhinovirus was the most common virus in coinfections while RSV plus rhinovirus/enterovirus were the most frequent combination. RSV and metapneumovirus showed a similar seasonal distribution to the influenza virus, which made it necessary to use a virological diagnostic assay during the influenza season.

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“…Multiplex respiratory panels are likely to allow the epidemiology of certain pathogens to be better defined. In an epidemiological analysis of 44,230 patients with respiratory illness, multiplex molecular testing identified that infection with human coronavirus was more common during the influenza season than previously recognized (47). Multiplex testing may lead to the diagnosis of some infections that have been commonly missed due to a lack of clinical suspicion or available routine testing.…”

Section: Advantages and Limitationsmentioning

confidence: 99%

Syndromic Panel-Based Testing in Clinical Microbiology

et al. 2018

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The recent development of commercial panel-based molecular diagnostics for the rapid detection of pathogens in positive blood culture bottles, respiratory specimens, stool, and cerebrospinal fluid has resulted in a paradigm shift in clinical microbiology and clinical practice. This review focuses on U.S. Food and Drug Administration (FDA)-approved/cleared multiplex molecular panels with more than five targets designed to assist in the diagnosis of bloodstream, respiratory tract, gastrointestinal, or central nervous system infections. While these panel-based assays have the clear advantages of a rapid turnaround time and the detection of a large number of microorganisms and promise to improve health care, they present certain challenges, including cost and the definition of ideal test utilization strategies (i.e., optimal ordering) and test interpretation.

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Extensive multiplex PCR diagnostics reveal new insights into the epidemiology of viral respiratory infections

Cited by 61 publications

References 37 publications

Outcomes of Nosocomial Viral Respiratory Infections in High-Risk Neonates

Outcomes of Nosocomial Viral Respiratory Infections in High-Risk Neonates

Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients

Syndromic Panel-Based Testing in Clinical Microbiology

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