Extended-Spectrum β-Lactamase CTX-M-1 in
            <i>Escherichia coli</i>
            Isolates from Healthy Poultry in France

Girlich, Delphine; Poirel, Laurent; Carattoli, Alessandra; Kempf, Isabelle; Lartigue, Marie‐Frédérique; Bertini, Alessia; Nordmann, Patrice

doi:10.1128/aem.02491-06

Cited by 137 publications

(135 citation statements)

References 47 publications

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“…The genes coding CTX-M group β-lactamase are harbored by plasmids as IS Ecp1. After determining the presence and transmitting by conjugation of ESBL type CTX-M groups in healthy foodproducing animals, this issue has become increasingly important (12,17).…”

Section: Discussionmentioning

confidence: 99%

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

Ünal¹,

Karagöz²,

Aşkar³

et al. 2017

Turk J Vet Anim Sci

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IntroductionEscherichia coli strains are important pathogens in animals and humans, and are normal commensals in intestinal tracts. The β-lactam group of antibiotics has been intensely used in human and veterinary medicine as infectious disease treatments (1). Extended-spectrum betalactamases (ESBLs) hydrolyze oxyimino-cephalosporins and give resistance to bacteria against the penicillins, cephalosporins (first, second, and third generations), and aztreonam. They are repressed by β-lactamase inhibitors. ESBLs are classified as β-lactamases containing three main families: TEM (named after the patient Temoneria), SHV (sulfhydryl reagent variable), and CTX-M (active on cefotaxime, first isolated in Munich) (2). The prevalence of CTX-M-type β-lactamase-producing E. coli has increased in poultry over the last decade (3).Increasing prevalence of ESBL-producing E. coli in poultry has become a major zoonotic risk because of possible transmission from broilers to humans (3). In addition, ESBL-producing E. coli strains that may have multidrug resistance (MDR) and cause therapeutic problems have been reported in human and veterinary practice (4). The alarming spread of ESBL-producing Enterobacteriaceae poses a serious public health threat and has attracted the attention of scientists, politicians, and the general public globally. There was no previous study about the presence, prevalence, and molecular epidemiology of ESBL-producing E. coli isolates from broilers in Turkey. The aim of the present study was to investigate the resistance phenotype and genotyping characteristics of ESBL-carrying E. coli strains in healthy broilers in Turkey. Materials and methods Samples, isolations, and identificationFrom January to April 2012, 300 samples were collected from cloacal samples of healthy slaughterhouse broilers in two regions of Turkey. Broiler cloacal samples were obtained from a total of 30 different poultry houses

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Section: Discussionmentioning

confidence: 99%

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

Ünal¹,

Karagöz²,

Aşkar³

et al. 2017

Turk J Vet Anim Sci

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“…Failure of conjugation in the present study may be because of the small size plasmids carried by the donors. It is suggestive to study the involvements of the insertion sequence ISEcp1 [37,38], as well as assessment of the incompatibility group [39,40] of the plasmid may help to understand the failure.…”

Section: Discussionmentioning

confidence: 99%

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

et al. 2013

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Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extendedspectrum b-lactamases (ESBLs). A total of 138 enteric bacteria were isolated from 53 faecal samples of pigs collected from different districts of Mizoram, of which 102 (73.91 %) were Escherichia coli, 26 (18.84 %) were Salmonella spp. and 10 (7.25 %) were Klebsiella pneumoniae. Phenotypic confirmatory test (Double Discs Synergy Test) showed that 8 (5.80 %) E. coli isolates were ESBLs producer. PCR analysis confirmed that out of the eight isolate, 7 (5.07 %) harboured bla CTX-M-1 gene and/or bla TEM gene. Of the eight positive isolates, 7 (5.07 %) and 3 (2.17 %) were found to be positive for bla CTX-M-1 gene and bla TEM gene, respectively, of which 3 (2.17 %) isolates were positive for both the genes. Only 4 (2.90 %) E. coli isolates carried bla CTX-M-1 gene alone. Agarose gel electrophoresis showed that all the isolates were carrying plasmids ranging between 0.9 and *30 kb. Out of the seven isolates positive for bla CTX-M-1 and/or bla TEM , 2 (1.84 %) isolates were confirmed for bla CTX-M-1 gene in their plasmid. Only one E. coli isolate was found to be positive for both the genes in its plasmid. The resistance plasmid could not be transferred to a recipient by in vitro horizontal gene transfer method.

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“…This may result in a selective advantage and a consequent increase in the abundance of resistant bacteria in animals, their wastes and surrounding environment [5]. Antibiotic resistant bacteria have been detected in poultry waste [7,8,6,9], commercial poultry production environments [10,11] and poultry products [12,13].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria

Adelowo

Fagade

Agersø

2014

J Infect Dev Ctries

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Introduction: This study investigated the mechanisms of resistance in 36 E. coli isolated from waste, litter, soil and water samples collected from poultry farms in Southwestern Nigeria. Methodology: Minimum inhibitory concentration (MIC) distributions of the isolates were determined using the methods of the Clinical and Laboratory Standard Institute and resistance genes detected by PCR. Results: A total of 30 isolates (94%) showed resistance to more than one antimicrobial. Percentage resistance was: tetracycline 81%, sulphamethoxazole 67%, streptomycin 56%, trimethoprim 47 %, ciprofloxacin 42%, ampicillin 36%, spectinomycin 28%, nalidixic acid 25%, chloramphenicol 22%, neomycin 14%, gentamicin 8%, amoxicillin-clavulanate, ceftiofur, cefotaxime, colistin, florfenicol and apramycin 0%. Resistance genes found among the isolates include bla-TEM (85%), sul2 (67%), sul3 (17%), aadA (65%), strA (70%), strB (61%), catA1 (25%), cmlA1 (13%), tetA (21%) and tetB (17%). Class 1 and 2 integrons were found in five (14%) and six (17%) isolates, respectively, while one isolate was positive for both classes of integrons. Seven out of eight isolates with resistance to ciprofloxacin and MIC ≤ 32 mg/L to nalidixic acid contained qnrS genes. Conclusions: Our findings provided additional evidence that the poultry production environment in Nigeria represents an important reservoir of antibiotic resistance genes such as qnrS that may spread from livestock production farms to human populations via manure and water.

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Extended-Spectrum β-Lactamase CTX-M-1 in Escherichia coli Isolates from Healthy Poultry in France

Cited by 137 publications

References 47 publications

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

PCR-Based Detection of Extended-Spectrum β-Lactamases (bla CTX-M-1 and bla TEM ) in Escherichia coli, Salmonella spp. and Klebsiella pneumoniae Isolated from Pigs in North Eastern India (Mizoram)

Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria

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