Extended-spectrum cephalosporin-resistant gram-negative organisms in livestock: An emerging problem for human health?

Seiffert, Salome Nadja; Hilty, Markus; Perreten, Vincent; Endimiani, Andrea

doi:10.1016/j.drup.2012.12.001

Cited by 250 publications

(242 citation statements)

References 252 publications

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“…Their broadspectrum ability combined with low toxicity in comparison with other antimicrobials makes these antimicrobials ideal for human and veterinary medicine (Seiffert et al, 2013). For the majority of bacteria, the cell wall requires enzymatic linkage of pentapeptid precursor molecules into the peptidoglycan cell wall in order to synthesise.…”

Section: ß-Lactamsmentioning

confidence: 99%

“…There are a number of different resistance mechanisms associated with β-lactam resistance. The most important mechanism associated with β-lactam resistance in gram-negative bacteria such as E. coli, involves the inactivation of the antimicrobial by the production of β-lactamase enzymes that are capable of hydrolysing and inactivating the ß-lactam ring (Allocati et al, 2013;Bradford, 2001;Bush, 2010;Bush, 2012;Li et al, 2007;Seiffert et al, 2013;Sosa, 2009). Resistance may also occur due to mutations in the PBPs, decreased outer membrane permeability and multidrug efflux pumps (Babic et al, 2006;Bush, 2010;Woodford et al, 2007).…”

Section: ß-Lactamsmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Section: ß-Lactamsmentioning

confidence: 99%

Section: ß-Lactamsmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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“…Seftriakson için söz edilen tüm bu yan etkilerin toplam görülme olasılığı %3.7'dir. Seftriakson penisiline yüksek dirençli S. pneumoniae enfeksiyonlarında, dirençli gonokok enfeksiyonların-da, MRSA enfeksiyonlarında, K. oxytoca veya Pseudomonas enfeksiyonlarında, hastanedeki nazokomiyal enfeksiyonlarda, ESBL (genişlemiş spektrumlu beta laktamaz üreten) pozitif gram negatif bakterilerin enfeksiyonlarında, anaerop enfeksiyonlarda, VRE enfeksiyonlarında etkisizdir (10,11) . Günü-müzde özellikle ayaktan vakaların bakteriyel alt solunum yolu enfeksiyonlarında, pnömoni veya bronkopnömonilerinde, ağır veya komplike otit veya sinüzit vakalarında, deri, kemik veya eklem enfeksiyonlarında, üriner sistem enfeksiyonlarında, dirençli olmayan gonore vakalarında, meningokoksemi tedavisinde, bakteriyel menenjit tedavisinde kullanılır.…”

Section: Günümüzde Sefalosporinler Ve Antibiyotik Direnciunclassified

Cephalosporin Antibiotics and Antibiotic Rezistance in Three Decades

Yıldız¹,

Varkal²,

Ünüvar³

2015

Cocuk Dergisi

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“…Outros micro-organismos, como Klebsiella pneumoniae, Salmonella, Staphylococcus aureus, Enterococcus e Acinetobacter também foram estudados (HALSTED; ABID; DOWZICKY, 2007;HIROI et al, 2012;SEIFFERT et al, 2013;SORAAS et al, 2013;EGERVARN et al, 2014;PATTERNEL et al, 2014;BENINATI et al, 2015;CLEMENTE et al, 2015). Observou-se entre os resultados, ainda, que somente 8% (8/98) dos artigos não utilizou nenhuma técnica de biologia molecular, revelando que tais técnicas representam o presente e o futuro das pesquisas com micro-organismos resistentes no Brasil e no mundo.…”

Section: Introductionunclassified

PRESENÇA DE Escherichia coli PRODUTORA DE BETA-LACTAMASE DE ESPECTRO ESTENDIDO EM ALIMENTOS: ESTUDO PROSPECTIVO

Leite

Guimarães

2016

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RESUMOEsse artigo objetiva realizar uma prospecção dos trabalhos científicos já publicados a respeito da presença de Escherichia coli produtora de beta-lactamase de espectro estendido (ESBL) em alimentos. Para tanto, analisaram-se 21 combinações das palavras-chave E. coli, beta-lactamase, ESBL, food, food of animal origin e product of animal origin, nas plataformas Web of Science, ScienceDirect, Scopus e Scielo, a fim de identificar a combinação que originasse 100 resultados que mais se aproximassem do objetivo da pesquisa. Selecionou-se para análise 124 resultados obtidos com a pesquisa das palavras-chave ESBL, E. coli e food na plataforma Scopus. Identificou-se que as pesquisas neste tema têm aumentado nos últimos seis anos e estão concentradas especialmente na Europa e China. As técnicas de biologia molecular foram as mais frequentemente utilizadas, sendo necessário intensificar o seu uso no Brasil para aumentar o número de publicações brasileiras em periódicos de alto impacto, incrementando, assim, a sua visibilidade internacional.Palavras-chave: E. coli. ESBL.Alimentos. ABSTRACTThis article aims to conduct a survey of scientific papers published about the presence of betalactamase extended spectrum (ESBL)-producing Escherichia coli in food. Therefore, 21 combinations of keywords E. coli, beta-lactamase, ESBL, food, food of origin animal or product of animal origin in the Web of Science, ScienceDirect, Scopus and Scielo platforms were analyzed, in order to identify the combination that originated 100 results that most closely approached the objective of the research. One hundred twenty-four results obtained with the search of keywords ESBL, E. coli and food in Scopus platform were selected for analysis. It was identified that research in this area has increased in the last six years and are concentrated especially in Europe and China. Molecular biology techniques were the most frequently used, being necessary to step up its use in Brazil to increase the number of Brazilian publications in high impact journals, increasing thus its international visibility.

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Extended-spectrum cephalosporin-resistant gram-negative organisms in livestock: An emerging problem for human health?

Cited by 250 publications

References 252 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Cephalosporin Antibiotics and Antibiotic Rezistance in Three Decades

PRESENÇA DE Escherichia coli PRODUTORA DE BETA-LACTAMASE DE ESPECTRO ESTENDIDO EM ALIMENTOS: ESTUDO PROSPECTIVO

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