Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice

Abstract

E3 ubiquitin ligases are involved in many processes, regulating the response to biotic and abiotic stresses. In this study, 11 E3 ubiquitin ligase genes from Arabidopsis, which were hypothesized to function in response to biotic or abiotic stresses were selected, and the homologous genes in rice were found. Their functions were analyzed in rice. These 11 E3 ubiquitin ligase genes showed different patterns of expression under different treatments. The BMV:OsPUB39-infiltrated seedlings showed decreased resistance to Magnaporthe grisea (M. grisea) when compared with BMV:00-infiltrated seedlings, whereas the BMV:OsPUB34- and BMV:OsPUB33-infiltrated seedlings showed increased resistance. The involvement of these genes in the resistance against M. grisea may be attributed to the regulation of the accumulation of reactive oxygen species (ROS) and expression levels of defense-related genes. Seedlings infiltrated by BMV:OsATL69 showed decreased tolerance to drought stress, whereas BMV:OsPUB33-infiltraed seedlings showed increased tolerance, possibly through the regulation of proline content, sugar content, and expression of drought-responsive genes. BMV:OsATL32-infiltrated seedlings showed decreased tolerance to cold stress by regulating malondialdehyde (MDA) content and the expression of cold-responsive genes.

GhVIM28, a negative regulator identified from VIM family genes to depredate protein systematically in cotton under salt stress

“…It was reported that 11 E3 ubiquitin ligase genes in rice showed different expression patterns under different treatments [31].In our study, 29 GhVIM genes exhibited different expression patterns under various treatments. For instance, GhVIM2, GhVIM8, GhVIM19, GhVIM23, and GhVIM29 showed higher expression levels in stems, while GhVIM13 and GhVIM23 exhibited higher expression levels in roots.…”

Tripartite motif family – its role in tumor progression and therapy resistance: a review

“…Zinc finger motifs can be seen there. The RING finger domain is a feature of E3 ubiquitin ligases that mediates the transfer of ubiquitin to heterologous substrates as well as to itself [6]. Ubiquitination is a dynamic and reversible process in biology.…”

ATL Protein Family: Novel Regulators in Plant Response to Environmental Stresses

“…Modulation of the response to environmental stressors by OsATL69 and OsATL32 is contingent upon the presence of ABA. 121 In contrast, AtATL80 122 in Arabidopsis and OsATL38 105 in rice function as negative regulators of cold-stress tolerance. Furthermore, ATL80 was implicated in the negative regulation of Pi mobilization.…”

“…Similarly, silencing OsATL32 in rice plants led to reduced cold stress tolerance, potentially affecting the MDA level and the expression of genes associated with the cold response. Modulation of the response to environmental stressors by OsATL69 and OsATL32 is contingent upon the presence of ABA …”