ATL Protein Family: Novel Regulators in Plant Response to Environmental Stresses

Wu, Ming; Musazade, Elshan; Yang, Xiao; Yin, Le; Zhao, Zizhu; Zhang, Yu; Lu, Jingmei; Guo, Liquan

doi:10.1021/acs.jafc.3c05603

Cited by 4 publications

(1 citation statement)

References 174 publications

(349 reference statements)

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“…A large number of differentially expressed genes were identified from RNA-Seq data. Given the importance of ARABIDOPSIS TOXICOS EN LEVADURAs (ATLs) in plant response to environmental stresses ( Wu et al., 2023 ), one upregulated transcript, PsATL33 , was selected for functional characterization. We hypothesized that this upregulation may indicate a crucial role of PsATL33 in the regulation of cold-induced tree peony bud dormancy release.…”

Section: Introductionmentioning

confidence: 99%

A tree peony RING-H2 finger protein, PsATL33, plays an essential role in cold-induced bud dormancy release by regulating gibberellin content

Mao,

Yuan,

Gao

et al. 2024

Front. Plant Sci.

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Bud dormancy is crucial for woody perennial plants to resist low-temperature stress in winter. However, the molecular regulatory mechanisms underlying bud dormancy release are largely unclear. Here, a tree peony (Paeonia suffruticosa) transcript ARABIDOPSIS TOXICOS EN LEVADURA 33 (PsATL33), encoding a RING-H2 finger protein, was selected from previously generated RNA sequencing data of chilling-treated buds. The objective of this study is to investigate the role of PsATL33 in the regulation of cold-induced bud dormancy release. Subcellular localization assay revealed that PsATL33 was localized to the nucleus and plasma membrane. Reverse transcription–quantitative PCR analysis showed that PsATL33 was dramatically upregulated during cold-triggered bud dormancy release. Exogenous treatments with gibberellin (GA3) increased, but abscisic acid (ABA) inhibited the transcription of PsATL33. Ectopic transformation assay indicated that overexpression of PsATL33 in petunia promoted seed germination, plant growth, and axillary bud break. Silencing of PsATL33 in tree peony through virus-induced gene silencing assay delayed bud dormancy release. tobacco rattle virus (TRV)-PsATL33-infected buds exhibited reduced expression levels of dormancy break-related genes EARLY BUD-BREAK 1 (PsEBB1) and CARBOXYLESTERASE 15 (PsCXE15). Silencing of PsATL33 decreased the accumulation of bioactive GAs, GA1 and GA3, rather than ABA. Transcript levels of several genes involved in GA biosynthesis and signaling, including GA20-OXIDASE 1 (PsGA20ox1), GA3-OXIDASE 1 (PsGA3ox1), PsGA3ox3, GA2-OXIDASE 1 (PsGA2ox1), and GA-INSENSITIVE 1A (PsGAI1A), were changed by PsATL33 silencing. Taken together, our data suggest that PsATL33 functions as a positive regulator of cold-induced bud dormancy release by modulating GA production.

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Section: Introductionmentioning

confidence: 99%

A tree peony RING-H2 finger protein, PsATL33, plays an essential role in cold-induced bud dormancy release by regulating gibberellin content

Mao,

Yuan,

Gao

et al. 2024

Front. Plant Sci.

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Energy difference-driven ROS reduction for electrochemical tracking crop growth sensitized with electron-migration nanostructures

Zhang,

Zhou,

Wang

et al. 2024

Analytica Chimica Acta

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OsATL32 ubiquitinates the reactive oxygen species‐producing OsRac5–OsRbohB module to suppress rice immunity

Yan,

Wang,

et al. 2024

JIPB

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Ubiquitination‐mediated protein degradation is integral to plant immunity, with E3 ubiquitin ligases acting as key factors in this process. Here, we report the functions of OsATL32, a plasma membrane‐localized Arabidopsis Tóxicos En Levadura (ATL)‐type E3 ubiquitin ligase, in rice (Oryza sativa) immunity and its associated regulatory network. We found that the expression of OsATL32 is downregulated in both compatible and incompatible interactions between rice and the rice blast fungus Magnaporthe oryzae. The OsATL32 protein level declines in response to infection by a compatible M. oryzae strain or to chitin treatment. OsATL32 negatively regulates rice resistance to blast and bacterial leaf blight diseases, as well as chitin‐triggered immunity. Biochemical and genetic studies revealed that OsATL32 suppresses pathogen‐induced reactive oxygen species (ROS) accumulation by mediating ubiquitination and degradation of the ROS‐producing OsRac5–OsRbohB module, which enhances rice immunity against M. oryzae. The protein phosphatase PHOSPHATASE AND TENSIN HOMOLOG enhances rice blast resistance by dephosphorylating OsATL32 and promoting its degradation, preventing its negative effect on rice immunity. This study provides insights into the molecular mechanism by which the E3 ligase OsATL32 targets a ROS‐producing module to undermine rice immunity.

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ATL Protein Family: Novel Regulators in Plant Response to Environmental Stresses

Cited by 4 publications

References 174 publications

A tree peony RING-H2 finger protein, PsATL33, plays an essential role in cold-induced bud dormancy release by regulating gibberellin content

A tree peony RING-H2 finger protein, PsATL33, plays an essential role in cold-induced bud dormancy release by regulating gibberellin content

Energy difference-driven ROS reduction for electrochemical tracking crop growth sensitized with electron-migration nanostructures

OsATL32 ubiquitinates the reactive oxygen species‐producing OsRac5–OsRbohB module to suppress rice immunity

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