Expression pattern of placenta specific 8 and keratin 20 in different types of gastrointestinal cancer

Abstract

The aim of the present study was to investigate the expression of keratin 20 (KRT20) and placenta specific 8 (PLAC8) in gastrointestinal (GI) cancer with various differentiation phenotypes. The present study retrospectively investigated archived formalin-fixed paraffin-embedded tissue samples from 12 patients at different stages of GI cancer [four with gastric cancer, four with pancreatic cancer and four with colorectal cancer (crc)]. The stages were pre-determined, according to differentiation phenotypes, by a pathologist of the Department of Pathology at Sijhih Cathay General Hospital. KRT20 and PLAC8 expression levels were assessed using immunohistochemistry. The crc cell lines SW620 and caco-2 were used to assess interactions between KRT20 and PLAC8 via reverse transcription-quantitative Pcr. PLAC8 and KRT20 expression was observed consistently only in the well-differentiated CRC tissue samples. Low KRT20 expression levels were observed in the PLAC8 knockdown SW620 cells. In addition, there was a positive association between PLAC8 and KRT20 expression in the differentiated caco-2 cells. according to the results of the present study, the differentiation status of GI cancer influenced KrT20 expression, particularly in crc, which may explain why patients with well-differentiated CRC display better clinical outcomes. Therefore, the prognostic significance of KrT20 and PLAC8 may be particularly crucial for patients with CRC displaying a well-differentiated phenotype.

A gut butyrate‑producing bacterium <em>Butyricicoccus&nbsp;pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

“…no. 4368813; Thermo Fisher Scientific, Inc.), as previously described ( 35 ). To quantify the expression of GPR43, the reaction mixture containing the cDNA sample, QuantiTect SYBR-Green PCR Master mix (Qiagen GmbH), QuantiTect Primer assay (cat.…”

Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq

“…After the organoids were generated, we did not observe differences in growth and morphology by bright-field microscopy. We assessed the expression of the homeobox protein CDX2, which is a transcription factor responsible for the differentiation and maintenance of the intestinal phenotype 16 and keratin 20 (CK20), whose expression is observed consistently higher in the well‑differentiated CRC tissue samples 17 (Fig. 2c and Supplementary Fig.…”

Functional Plasmon-Activated Water Increases Akkermansia muciniphila Abundance in Gut Microbiota to Ameliorate Inflammatory Bowel Disease

“…muciniphila ; this finding accords with that reported in previous studies specifically, that PAW has therapeutic potential for inflammatory diseases [ 12 ]. CK20 and COX-2 are critical in the development and progression of CRC [ 56 , 57 , 58 ]. Collectively, the negative results of CK20 and COX-2 and the IHC results for IL-6 and TNF-α indicate that PAW does not increase tumorigenicity.…”