Expression of yeast Hem1 gene controlled by Arabidopsis HemA1 promoter improves salt tolerance in Arabidopsis plants

Zhang, Zhi Ping; Yao, Quan Hong; Wang, Liang Ju

doi:10.5483/bmbrep.2010.43.5.330

Cited by 21 publications

(12 citation statements)

References 20 publications

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“…Transgenic rice plants with overexpressed M. xanthus PPOX gene were characterized by more stable Fv/Fm, lower concentration of H 2 O 2 and less MDA compared with the wild-type under incubated oxyfluorfen stress (0.1-10 µmol/L), and this indicated that the transgenic lines of rice had enhanced resistance (Jung and Back 2005). Transgenic Arabidopsis plants with yeast ALAS gene (YHem1) showed higher resistance when exposed to NaCl stress, their germination, growth, chlorophyll and heme contents were much higher than the wild type (Zhang et al 2010). Besides, in the study of transgenic rice, which overexpressed B. japonicum FECH gene, resulted in increasing activity of FECH, then raising content of heme and enhancing the tolerance of oxidative stress (Kim et al 2014).…”

Section: Manipulation Of Relative Genes In Ala Pathwaymentioning

confidence: 99%

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

Liao

Dawuda

et al. 2018

Plant Growth Regul

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Crop productivity is restricted by various abiotic stresses such as drought, salinity, heat, and cold. Many efforts have been taken to decrease the inhibition of plant growth by alleviating the abiotic stresses. Exogenous applications of hormones, plant growth regulators, and/or small signaling molecules have been reported as a means to enhance plant resistance to stress. One of the small signaling molecules utilized is 5-aminolevulinic acid (ALA) that has been shown to enhance plant growth under abiotic stress. As a metabolic intermediate in higher plants, ALA is a precursor of all tetrapyrroles such as chlorophyll, heme and siroheme. The pathway towards biosynthesis upstream and the metabolism downstream of ALA contains multiple regulatory points that are affected by positive/negative factors. However, report about the regulatory aspects of the ALA metabolic pathway and the role of ALA in stimulating physiochemical processes in higher plants under stress have not been collated and summarized systematically. In this regard, we summarize recent developments in understanding the mechanisms of plant responses to abiotic stress which are affected by ALA as well as new information on the metabolic pathway of ALA. We find that exogenous application of ALA can enhance some key physiological and biochemical processes in plants such as photosynthesis, nutrient uptake, antioxidant characteristics and osmotic equilibrium, however, more in-depth research on the specific mechanisms are needed.

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Section: Manipulation Of Relative Genes In Ala Pathwaymentioning

confidence: 99%

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

Liao

Dawuda

et al. 2018

Plant Growth Regul

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“…Arabidopsis ( Arabidopsis thaliana ) of wild-type (Col-0) and ALA-over-producing transgenic lines ( YHem1 ; Zhang et al, 2010 ) that derived from Col-0 background were used in this study. Seeds were surface sterilized with bleaching power (5%, w/v) for 20 min, washed with sterilized water three times, then germinated and grown on vermiculite.…”

Section: Methodsmentioning

confidence: 99%

“…5-Aminolevulinic acid-overproducing transgenic Arabidopsis ( YHem1 ) have been obtained by expressing yeast Hem1 gene under the control of Arabidopsis HemA1 promoter ( Zhang et al, 2010 ). To test our hypothesis, here, we first investigated whether ALA inhibited ABA-induced stomatal closure.…”

Section: Introductionmentioning

confidence: 99%

ALA Inhibits ABA-induced Stomatal Closure via Reducing H2O2 and Ca2+ Levels in Guard Cells

Liu

Chen

et al. 2016

Front. Plant Sci.

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5-Aminolevulinic acid (ALA), a newly proved natural plant growth regulator, is well known to improve plant photosynthesis under both normal and stressful conditions. However, its underlying mechanism remains largely unknown. Stomatal closure is one of the major limiting factors for photosynthesis and abscisic acid (ABA) is the most important hormone in provoking stomatal closing. Here, we showed that ALA significantly inhibited ABA-induced stomatal closure using wild-type and ALA-overproducing transgenic Arabidopsis (YHem1). We found that ALA decreased ABA-induced H2O2 and cytosolic Ca2+ accumulation in guard cells with stomatal bioassay, laser-scanning confocal microscopy and pharmacological methods. The inhibitory effect of ALA on ABA-induced stomatal closure was similar to that of AsA (an important reducing substrate for H2O2 removal), CAT (a H2O2-scavenging enzyme), DPI (an inhibitor of the H2O2-generating NADPH oxidase), EGTA (a Ca-chelating agent), and AlCl3 (an inhibitor of calcium channel). Furthermore, ALA inhibited exogenous H2O2- or Ca2+-induced stomatal closure. Taken together, we conclude that ALA inhibits ABA-induced stomatal closure via reducing H2O2, probably by scavenging, and Ca2+ levels in guard cells. Moreover, the inhibitive effect of ALA on ABA-induced stomatal closure was further confirmed in the whole plant. Finally, we demonstrated that ALA inhibits stomatal closing, but significantly improves plant drought tolerance. Our results provide valuable information for the promotion of plant production and development of a sustainable low-carbon society.

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“…Arabidopsis ( Arabidopsis thaliana ) of wild-type (Col-0, WT), ALA-over-producing transgenic lines ( YHem1 ) (Zhang et al, 2010) and CHS tt4 mutant that derived from Col-0 background were used in this study. Seeds were surface sterilized with bleaching power (5%, w/v) for 20 min, washed with sterilized water three times, then germinated and grown on Murashige and Skoog (MS) medium.…”

Section: Methodsmentioning

confidence: 99%

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Feng

Liu

et al. 2016

Front. Plant Sci.

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5-aminolevulinic acid (ALA), a new plant growth regulator, can inhibit stomatal closure by reducing H2O2 accumulation in guard cells. Flavonols are a main kind of flavonoids and have been proposed as H2O2 scavengers in guard cells. 5-aminolevulinic acid can significantly improve flavonoids accumulation in plants. However, whether ALA increases flavonols content in guard cells and the role of flavonols in ALA-regulated stomatal movement remains unclear. In this study, we first demonstrated that ALA pretreatment inhibited ABA-induced stomatal closure by reducing H2O2 accumulation in guard cells of Arabidopsis seedlings. This result confirms the inhibitory effect of ALA on stomatal closure and the important role of decreased H2O2 accumulation in this process. We also found that ALA significantly improved flavonols accumulation in guard cells using a flavonol-specific dye. Furthermore, using exogenous quercetin and kaempferol, two major components of flavonols in Arabidopsis leaves, we showed that flavonols accumulation inhibited ABA-induced stomatal movement by suppressing H2O2 in guard cells. Finally, we showed that the inhibitory effect of ALA on ABA-induced stomatal closure was largely impaired in flavonoid-deficient transparent testa4 (tt4) mutant. In addition, exogenous flavonols recovered stomatal responses of tt4 to the wild-type levels. Taken together, we conclude that ALA-induced flavonol accumulation in guard cells is partially involved in the inhibitory effect of ALA on ABA-induced H2O2 accumulation and stomatal closure. Our data provide direct evidence that ALA can regulate stomatal movement by improving flavonols accumulation, revealing new insights into guard cell signaling.

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Expression of yeast Hem1 gene controlled by Arabidopsis HemA1 promoter improves salt tolerance in Arabidopsis plants

Cited by 21 publications

References 20 publications

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

ALA Inhibits ABA-induced Stomatal Closure via Reducing H2O2 and Ca2+ Levels in Guard Cells

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

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