ALA Inhibits ABA-induced Stomatal Closure via Reducing H2O2 and Ca2+ Levels in Guard Cells

An, Yuyan; Liu, Longbo; Chen, Linghui; Wang, Liangju

doi:10.3389/fpls.2016.00482

Cited by 40 publications

(77 citation statements)

References 57 publications

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“…5-Aminolevulinic acid (ALA), a natural plant growth regulator, is known to improve plant photosynthesis under both normal (Hotta et al, 1997) and stressful conditions (Hotta et al, 1998; Nishihara et al, 2003; Wang et al, 2004; Liu et al, 2011; Zhang et al, 2012; Ali et al, 2013b). Recent investigations showed that ALA did not affect stomata development, but significantly inhibited stomatal closing (An et al, 2016a), indicating increasing stomatal aperture is an important mechanism of ALA-mediated improvement of photosynthesis. Most importantly, ALA improved plant drought tolerance simultaneously (An et al, 2016a), further suggesting its great application potential in agriculture and forestry.…”

Section: Introductionmentioning

confidence: 99%

“…Recent investigations showed that ALA did not affect stomata development, but significantly inhibited stomatal closing (An et al, 2016a), indicating increasing stomatal aperture is an important mechanism of ALA-mediated improvement of photosynthesis. Most importantly, ALA improved plant drought tolerance simultaneously (An et al, 2016a), further suggesting its great application potential in agriculture and forestry.…”

Section: Introductionmentioning

confidence: 99%

“…Hydrogen peroxide (H 2 O 2 ) is an important signaling molecule in plants and has been proved as an essential signal in ABA-induced stomatal closure (Pei et al, 2000; Rhee, 2006). We recently demonstrated that ALA treatment inhibited ABA-induced stomatal closure by reducing H 2 O 2 accumulation in guard cells (An et al, 2016a). These results revealed the interaction between ALA and ABA in regulating stomatal movement and indicated a crucial function of H 2 O 2 in this interaction.…”

Section: Introductionmentioning

confidence: 99%

“…Munemasa et al (2013) found that depletion of glutathione contributed to a higher level of ABA-induced H 2 O 2 accumulation, indicating glutathione is also a H 2 O 2 scavenger in ABA signaling. Our previous study suggested that ALA reduced H 2 O 2 in guard cells mainly through accelerating its elimination (An et al, 2016a). However, until now, little is known about how ALA scavenges H 2 O 2 in guard cells.…”

Section: Introductionmentioning

confidence: 99%

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ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Feng

Liu

et al. 2016

Front. Plant Sci.

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5-aminolevulinic acid (ALA), a new plant growth regulator, can inhibit stomatal closure by reducing H2O2 accumulation in guard cells. Flavonols are a main kind of flavonoids and have been proposed as H2O2 scavengers in guard cells. 5-aminolevulinic acid can significantly improve flavonoids accumulation in plants. However, whether ALA increases flavonols content in guard cells and the role of flavonols in ALA-regulated stomatal movement remains unclear. In this study, we first demonstrated that ALA pretreatment inhibited ABA-induced stomatal closure by reducing H2O2 accumulation in guard cells of Arabidopsis seedlings. This result confirms the inhibitory effect of ALA on stomatal closure and the important role of decreased H2O2 accumulation in this process. We also found that ALA significantly improved flavonols accumulation in guard cells using a flavonol-specific dye. Furthermore, using exogenous quercetin and kaempferol, two major components of flavonols in Arabidopsis leaves, we showed that flavonols accumulation inhibited ABA-induced stomatal movement by suppressing H2O2 in guard cells. Finally, we showed that the inhibitory effect of ALA on ABA-induced stomatal closure was largely impaired in flavonoid-deficient transparent testa4 (tt4) mutant. In addition, exogenous flavonols recovered stomatal responses of tt4 to the wild-type levels. Taken together, we conclude that ALA-induced flavonol accumulation in guard cells is partially involved in the inhibitory effect of ALA on ABA-induced H2O2 accumulation and stomatal closure. Our data provide direct evidence that ALA can regulate stomatal movement by improving flavonols accumulation, revealing new insights into guard cell signaling.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Feng

Liu

et al. 2016

Front. Plant Sci.

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“…Furthermore, the increase in ABA level is a central player controlling downstream responses essential for adaptation to different stresses, especially drought (Davies et al, 2002 andGiuliani et al, 2005). These responses include changes in stomatal conductance (closure of stomata), osmolyte accumulation, gene expression, a reduced leaf canopy, deeper root growth and changes in root system architecture (Davies et al, 2002;Giuliani et al, 2005;Lee et al, 2013 andAn et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Study of the Interactive Effects of Calcium and Abscisic Acid on Drought Stressed Triticum aestivum Seedlings

Sewelam

2017

Egypt. J. Bot.

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IN THEIR natural habitats, plants are continuously subjected to many severe environmental challenges including drought. In this study, the effects of drought on Triticum aestivum seedlings were assessed, and the alleviating roles and interactive effects of calcium (Ca 2+ ) and abscisic acid (ABA) to drought stress were studied. The results showed that pretreating wheat seedlings with calcium chloride (CaCl 2 ) or ABA led to alleviating most of the negative effects of 20% polyethylene glycol (PEG, as a drought imposing agent). Ca 2+ improved the growth criteria, photosynthetic activity, but ABA had no significant effects on growth parameters. Ca 2+ or ABA pretreatments have alleviated the toxic effects of the drought-induced oxidative stress which was marked with the reduction of MDA content. Ca 2+ and/or ABA reduced the PEGinduced catalase activity, and ascorbic acid content. The use of lanthanum chloride (LaCl 3 ) as a calcium channel blocker has confirmed the role of Ca 2+ in ameliorating the drought effects on plant. This study revealed that the application of Ca 2+ has increased the endogenous ABA level of the treated seedlings to a high extent exceeding that of PEG treatment itself suggesting that ABA requires Ca 2+ ions for its induction, and ABA is working downstream from Ca 2+ in the relevant signaling pathways activated by drought stress. The alleviating effects of Ca 2+ was prolonged to the yield stage. Overall, our results suggest that Ca 2+ (10 mM), and with a less extend ABA (0.1 mM), represent signaling agents that can partially alleviate drought stress in plants growing in dry environments.

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Morpho-Physiological, Biochemical, and Ultrastructural Modifications on Sugarcane to Prolonged Water Deficit

Song¹,

Rajput²,

Boldyreva³

et al. 2022

Agro-Industrial Perspectives on Sugarcane Production Under Environmental Stress

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ALA Inhibits ABA-induced Stomatal Closure via Reducing H2O2 and Ca2+ Levels in Guard Cells

Cited by 40 publications

References 57 publications

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Study of the Interactive Effects of Calcium and Abscisic Acid on Drought Stressed Triticum aestivum Seedlings

Morpho-Physiological, Biochemical, and Ultrastructural Modifications on Sugarcane to Prolonged Water Deficit

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