Expression of tapasin in rainbow trout tissues and cell lines and up regulation in a monocyte/macrophage cell line (RTS11) by a viral mimic and viral infection

Sever, Lital; Vo, Nguyen T. K.; Bols, Niels C.; Dixon, Brian

doi:10.1016/j.dci.2013.11.019

Cited by 22 publications

(13 citation statements)

References 41 publications

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“…Currently, several immortalized cells derived from various epithelial lining tissues are available for studying immune gene expression in trout (Bols et al, 1994;Kawano et al, 2011). Recently, the RTgutGC cell line from the intestinal epithelia of Oncorhynchus mykiss was shown to express several immune related molecules, such as TNF-α (Kawano et al, 2011), tapasin (Sever et al, 2014), MHC class I alpha chain and beta-2-microglobulin (Kawano et al, 2010), suggesting behavior comparable to IECs. Therefore, RTgutGC cells were considered a representative model that may be used to address the in vitro effect of zymosan in the intestinal immune response of the rainbow trout.…”

Section: Discussionmentioning

confidence: 95%

Immunomodulatory effect of cathelicidins in response to a β-glucan in intestinal epithelial cells from rainbow trout

Schmitt

Wacyk

Morales‐Lange

et al. 2015

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 95%

Immunomodulatory effect of cathelicidins in response to a β-glucan in intestinal epithelial cells from rainbow trout

Schmitt

Wacyk

Morales‐Lange

et al. 2015

Developmental & Comparative Immunology

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“…WE‐skin11f cells were kept at 26, 20, 14 or 4°C. Cells were harvested at 1, 2, 3, 5, 7 days post‐change in incubation temperature followed by total RNA extraction using the RNeasy kit (Qiagen), along with an on‐column DNaseI digestion (Invitrogen), according to the manufacturer's instructions as previously described (Sever, Vo, Bols, & Dixon, ). RNA was reverse transcribed to cDNA using the Quanta cDNA synthesis kit according to manufacturer's instructions, as previously described (Semple et al, ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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A walleye dermal fibroblastoid cell line, WE‐skin11f, was established and characterized. WE‐skin11f was immunocytochemically positive for two known dermal fibroblast protein markers: vimentin and collagen I. At passage 26, WE‐skin11f cultures contained both diploid and aneuploid populations. Ascorbic acid was required to produce extracellular collagen I fibres. Both of the skin fibroblastoid cell lines, WE‐skin11f and rainbow trout‐derived RTHDF, were not as good as the walleye caudal fin fibroblastoid cell line, WE‐cfin11f, at forming abundant dense extracellular collagen matrices. The thermobiology of WE‐skin11f was similar to that of other walleye cell lines with 26°C showing best temperature for growth and 4°C showing no growth but 100% viability. The transcript levels of b2m and mhIa genes of the major histocompatibility class I receptor in WE‐skin11f were largely similar at all temperatures examined (4, 14, 20 and 26°C). Cortisol had a variety of effects on WE‐skin11f cells: growth inhibition, morphological change from fibroblastoid to epithelioid, and enhancement of barrier function. Treatment of WE‐skin11f cells with the physiologically relevant concentration of 100 ng/ml cortisol inhibited collagen I synthesis and matrix formation. Thus, WE‐skin11f cell line could be useful in fish dermatology, endocrinology, and immunology research.

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“…Homogenates were prepared as previously described by Sever et al (2014) for Western blot analysis with mAb 6-11B-1 from the organs of a sexually mature female walleye. Tissue fragments of approximately 50-80 mg of caudal fin, skin, brain, retina, muscle, gill, spleen, bulbus arteriosus, heart, head kidney, ovary, posterior intestine and liver were placed in the microfuge tubes on ice.…”

Section: Organ Homogenatesmentioning

confidence: 99%

Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

Bols

2015

Fish Physiol Biochem

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Primary cilia (PC) were demonstrated for the first time in fish endothelial, epithelial and fibroblast cell lines through immunofluorescence staining with the monoclonal antibody, 6-11B-1, against acetylated α-tubulin. The study was carried out with eight recently developed cell lines from the walleye, Sander vitreus (Mitchill). These were three fibroblast-like cell lines, WE-cfin11f, WE-skin11f and WE-liver3 from, respectively, the caudal fin, skin and liver, and three epithelial-like cell lines, WE-cfin11e, WE-spleen6 and WErpe from, respectively, the caudal fin, spleen and retina. Also, endothelial-like WEBA from the bulbus arteriosus and glial-like WE-brain5 from the brain were used. Immunocytochemistry revealed strong staining for acetylated α-tubulin in mitotic spindles and midbodies for all cell lines, and in PC for all cell lines except WE-skin11f. Staining of cytoplasmic microtubules (fibrils) was absent in three cell lines, including WEBA, but present in the others, especially WE-skin11f, which might have obscured PC detection in these cells. Tubacin, an inhibitor of histone deacetylase 6, induced cytoplasmic fibrils in WEBA and the intensity of their staining in WE-cfin11f. These results suggest that the cell lines might differ in their deacetylase activities, making them useful for studying this tubulin modification in teleosts, as well as for studying PC.

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Expression of tapasin in rainbow trout tissues and cell lines and up regulation in a monocyte/macrophage cell line (RTS11) by a viral mimic and viral infection

Cited by 22 publications

References 41 publications

Immunomodulatory effect of cathelicidins in response to a β-glucan in intestinal epithelial cells from rainbow trout

Immunomodulatory effect of cathelicidins in response to a β-glucan in intestinal epithelial cells from rainbow trout

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

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