Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

Vo, Nguyen T. K.; Bols, Niels C.

doi:10.1007/s10695-015-0114-1

Cited by 11 publications

(7 citation statements)

References 65 publications

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“…As part of studying senescence (Vo, Mikhaeil, Lee, Pham, & Bols, ) and primary cilia in fish cell lines (Vo & Bols, ), we developed a walleye skin fibroblastoid cell line, WE‐skin11f, but did not characterize the cells further. WE‐skin11f is the first cell line from normal walleye skin and so further characterization is warranted.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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A walleye dermal fibroblastoid cell line, WE‐skin11f, was established and characterized. WE‐skin11f was immunocytochemically positive for two known dermal fibroblast protein markers: vimentin and collagen I. At passage 26, WE‐skin11f cultures contained both diploid and aneuploid populations. Ascorbic acid was required to produce extracellular collagen I fibres. Both of the skin fibroblastoid cell lines, WE‐skin11f and rainbow trout‐derived RTHDF, were not as good as the walleye caudal fin fibroblastoid cell line, WE‐cfin11f, at forming abundant dense extracellular collagen matrices. The thermobiology of WE‐skin11f was similar to that of other walleye cell lines with 26°C showing best temperature for growth and 4°C showing no growth but 100% viability. The transcript levels of b2m and mhIa genes of the major histocompatibility class I receptor in WE‐skin11f were largely similar at all temperatures examined (4, 14, 20 and 26°C). Cortisol had a variety of effects on WE‐skin11f cells: growth inhibition, morphological change from fibroblastoid to epithelioid, and enhancement of barrier function. Treatment of WE‐skin11f cells with the physiologically relevant concentration of 100 ng/ml cortisol inhibited collagen I synthesis and matrix formation. Thus, WE‐skin11f cell line could be useful in fish dermatology, endocrinology, and immunology research.

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Section: Introductionmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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“…Transmembrane proteins, polycystin-1 and polycystin-2, form a complex at the ciliary membrane to mediate calcium influx. Geerts et al, 2011;Egorova et al, 2012) and fluorescent confocal microscopy (Van der Heiden et al, 2006;Geerts et al, 2011;Russellpuleri et al, 2017;Nauli et al, 2008;Antal et al, 2017;Vo and Bols, 2016;Iomini et al, 2004;Ki et al, 2020;Dummer et al, 2018;Goetz et al, 2014). Transmission electron microscopy can provide the detailed ultrastructure information of the cilium and scanning electron microscopy can provide information regarding cell surface morphology, cilia density and the contact of the cilia with the cell surface.…”

Section: Dimensionsmentioning

confidence: 99%

Mechano-Sensing and shear stress-shielding by endothelial primary cilia: structure, composition, and function

YIN¹,

WANG²,

Fan³

et al. 2021

Biocell

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Primary cilium is an antenna-like and non-motile structure protruding from the apical surface of most mammalian cells including endothelial cells lining the inner side of all the blood vessels in our body. Although it has been over a century since primary cilia were discovered, the investigation about their mechano-sensing and other roles in maintaining normal functions of cardiovascular system has just started in recent years. This focused review aims to give an update about the current literature for the role of endothelial primary cilia in blood flow mechanosensing and shear stress-shielding. To do this, we first summarized the characteristic features of endothelial primary cilia in terms of structure, dimension, molecular composition, and mechanical properties (e.g., bending rigidity), which are the dominant factors for their functions in mechano-sensing and transduction, as well as vascular protection from the blood flow-induced wall shear stress. We also described the experimental techniques and mathematical models for determining the dimension and mechanical properties of the primary cilium. Then we reviewed the molecular mechanisms underlying mechano-sensing and transduction by endothelial primary cilia and the mathematical model prediction for their roles in redistribution and reduction of wall shear stresses. Finally, we briefly discussed the common cardiovascular diseases, e.g., atherosclerosis, hypertension, and aneurysm, due to defects and malfunction of endothelial primary cilia and suggested potential targets for therapeutic treatments.

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“…Tubulin and microtubules (MTs) are the largest cytoskeletal components ( 16 ), and posttranslational modifications of tubulin are found in all cells with MTs ( 17 , 18 ). For example, acetyl-α-tubulin regulates MT stabilization and cell morphology ( 19 , 20 ). In addition, the loss of acetyl-α-tubulin is associated with TGF-β-induced epithelial-mesenchymal transition and sulfur mustard-induced chronic airway remodeling ( 21 , 22 ).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‑29b‑3p promotes α‑tubulin deacetylation by targeting the interaction of matrix metalloproteinase‑9 with integrin β1 in nasal polyps

Liu

et al. 2021

Int J Mol Med

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Matrix metalloproteinase (MMP)-9 is a key enzyme responsible for extracellular matrix degradation and contributes to the progressive histological changes observed in lower respiratory tract infections. Integrin β1 and α-tubulin are potential MMP-9-interacting proteins, and microRNA (miR)-29b-3p can regulate MMP-9 expression. MMP-9 is highly expressed in chronic rhinosinusitis with nasal polyps (cRSwNPs), regardless of its effects on miR-29b-3p, integrin β1 and α-tubulin expression. In the present study, samples from 100 patients with cRSwNPs were examined via reverse transcription-quantitative PcR to assess the mRNA expression of miR-29b-3p, and western blotting was performed to assess the protein expression of MMP-2, MMP-9, acetyl-α-tubulin, integrin β1 and tissue inhibitor of metalloproteinase 1 (TIMP-1). A dual-luciferase reporter assay was used to verify the direct binding of miR-29b-3p and MMP-2/MMP-9. co-immunoprecipitation (co-IP) and GST pull-down assays showed that integrin β1 and α-tubulin were MMP-9-interacting proteins. Cell viability, apoptosis and inflammatory cytokine levels were determined via a Cell Counting Kit-8 assay, flow cytometry and ELISA, respectively. miR-29b-3p expression was found to be positively correlated with MMP-2 and MMP-9 expression. Whereas, TIMP-1 expression was negatively correlated with MMP-2 and MMP-9 expression. The dual-luciferase assay revealed that miR-29b-3p targeted the 3' untranslated region of MMP-2/MMP-9. The co-IP and GST pull-down assays showed that MMP-9 could directly bind to integrin β1 and indirectly bind to α-tubulin. Finally, the overexpression of miR-29b-3p decreased the expression of MMP-9 and increased the levels of acetyl-α-tubulin. By contrast, the knockdown of miR-29b-3p increased the expression of MMP-9 and decreased the levels of acetyl-α-tubulin. Additionally, MMP-9 expression was found to be negatively correlated with acetyl-α-tubulin expression. Of note, the expression of integrin β1 did not change following the overexpression and knockdown of MMP-9. Finally, the overexpression of miR-29b-3p not only decreased MMP-9 expression, but also alleviated lipopolysaccharide-induced inflammation in NP69 cells. The results showed that the downregulation of miR-29b-3p promoted α-tubulin deacetylation by increasing the number of MMP-9-integrin β1 complexes in cRSwNPs, thus targeting miR-29b-3p/MMP-9 may be a potential novel strategy for the clinical treatment of cRSwNPs.

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Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

Cited by 11 publications

References 65 publications

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Mechano-Sensing and shear stress-shielding by endothelial primary cilia: structure, composition, and function

Downregulation of miR‑29b‑3p promotes α‑tubulin deacetylation by targeting the interaction of matrix metalloproteinase‑9 with integrin β1 in nasal polyps

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