Expression of platelet-derived growth factor BB, erythropoietin and erythropoietin receptor in canine and feline osteosarcoma

Abstract

The discovery of expression of the erythropoietin receptor (EPO-R) on neoplastic cells has led to concerns about the safety of treating anaemic cancer patients with EPO. In addition to its endocrine function, the receptor may play a role in tumour progression through an autocrine mechanism. In this study, the expression of EPO, EPO-R and platelet-derived growth factor BB (PDGF-BB) was analysed in five feline and 13 canine osteosarcomas using immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR).EPO expression was positive in all tumours by IHC, but EPO mRNA was only detected in 38% of the canine and 40% of the feline samples. EPO-R was expressed in all samples by quantitative RT-PCR (RT-qPCR) and IHC. EPO-R mRNA was expressed at higher levels in all feline tumours, tumour cell lines, and kidney when compared to canine tissues. PDGF-BB expression was variable by IHC, but mRNA was detected in all samples. To assess the functionality of the EPO-R on tumour cells, the proliferation of canine and feline osteosarcoma cell lines was evaluated after EPO administration using an alamarBlue assay and Ki67 immunostaining. All primary cell lines responded to EPO treatment in at least one of the performed assays, but the effect on proliferation was very low indicating only a weak responsiveness of EPO-R. In conclusion, since EPO and its receptor are expressed by canine and feline osteosarcomas, an autocrine or paracrine tumour progression mechanism cannot be excluded, although in vitro data suggest a minimal role of EPO-R in osteosarcoma cell proliferation.

Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines

“…Three markers differed between COS_1186w and the tumor, although one marker failed to amplify in the tumor sample, but not in the cell line. A complete list of the obtained microsatellite data as first published in [ 35 ] is supplied in Supplementary Tables S2 and S3 . These lists can be helpful in future experiments to exclude cross contaminations.…”

“…DNA was sent to Laboklin (Bad Kissingen, Germany) for DNA fingerprinting determining 18/16 marker regions for dogs or cats, respectively [ 35 ].…”

The expression of PDGF-BB predicts curative effect in locally advanced esophageal squamous cell carcinoma treated by radiotherapy

“…To our knowledge, this is the first study of the expression of PDGF-BB in radiotherapy treated EC. PDGF-BB was previously reported to be significantly associated with many human tumors including colorectal cancer [10,11], pancreatic cancer [11][12][13], non-small cell lung cancer [14], renal cancer [15], ovarian cancer [16], liver carcer [17,18] and even osteosarcoma [19]. Current thinking is that overexpression of the PDGF family probably leads to a poor prognosis by modulating both hematopoiesis and tumor angiogenesis [20].…”

Effect of erythropoietin on the content of lipid peroxidation products in lymphocytes in experimental thermal injury

“…Keywords: thermal trauma, erythropoietin, lipid peroxidation products. Выбор концентрации ЭПО обусловлен его средним содержанием в сыворотке крови в физиологических условиях, а также многократным увеличением концентрации ЭПО в крови при патологии, например при гипоксии различного генеза, что предполагает реализацию эффектов ЭПО на клетки в соответствующих концентрациях -100, 1000 и 10 000% физиологического уровня [5,10].…”