Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines

Meyer, Florian R. L.; Walter, Ingrid

doi:10.3390/vetsci3020009

Cited by 9 publications

(9 citation statements)

References 52 publications

(85 reference statements)

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“…The present study describes a 6-year-old gelding diagnosed with osteosarcoma, which is in agreement with the literature descriptions of the condition in younger animals. Similar fi ndings are described in dogs, in which the tumor affect both young and older animals, however, the condition is described only in older animals in the feline species [16,18].…”

Section: Discussionsupporting

confidence: 75%

Sinonasal Osteosarcoma in a Horse

Leite

Fabris

Silva

et al. 2019

Acta Scientiae. Vet.

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Background: Osteosarcomas are common malignant bone tumors described in dogs, humans and cats. However, there are rare reports in horses. The tumor etiology has not been fully elucidated. Clinical signs are associated to the tumor size, location and growth characteristics. When located on the face, the most common findings are facial distortion, nasal discharge and inspiratory dyspnea. The aim of this study was to report an osteoblastic osteosarcoma in a horse, located on the right maxillary region with projections into the corresponding nostril.Case: A 6-year-old mixbreed saddle gelding, presenting bay coat was admitted to a University Hospital with a chronic sinusitis history. The animal was previously examined and treated by other veterinarians, who suspected of odontogenic maxillary sinusitis, and therefore a repulsion of the fourth premolar from the right maxilla hemiarcade was performed. However, an improvement of the clinical signs was not observed and subsequently the gelding was referred to a Veterinary Hospital. During physical examination the patient presented dyspnea, tachypnea and tachycardia. An increase on the face size was observed, together with a fetid and purulent nasal discharge. During oral cavity inspection, a diagonal wear was detected at the occlusal surface of the right hemiarcade; food accumulation was seen at the dental extraction site, and a communication with the rostral maxillary sinus was evidenced, from where a purulent fetid discharge was draining. At the radiographic exam, well defined margins of a tumor were observed, with adjacent bone lysis and the presence of a central nucleus showing a gross granular mineral radiopacity, distorting the frontal and nasal bones. During the endoscopic exam, a mass was partially occluding the right nasal cavity close to the nasal opening, which was blocking the progression of the endoscopy. However, during the left cavity inspection, at the end of the nasal septum a mass with irregular surface was detected emerging from the border of the right choana, which extended up to the nasopharynx region. The diagnosis was established based on clinical evaluation and histopathological findings of the tumor, which confirmed osteoblastic osteosarcoma. The animal was euthanized due to poor clinical conditions and prognosis. There was no evidence of metastasis to other organs during necropsy.Discussion: The majority of reported osteosarcoma cases in horses do not define the tumor histopathological subtype. The occurrence of metastasis in equine osteosarcoma is not well established, however it seems to be uncommon. It is important to emphasize the relevance of performing a necropsy in patients presenting osteosarcoma, in order to establish a pattern concerning the metastasis incidence in the species. The predominant osteosarcoma location is the mandible, although there are few reports in the paranasal sinuses and appendicular skeleton. The tumor usual location makes the treatment difficult because of the impossibility of performing a complete surgical resection, which leads to a euthanasia decision. Although osteosarcoma has been little reported in horses, it should be a differential diagnosis for facial alterations and paranasal sinuses abnormalities, which would contribute to an early diagnosis and increase the chances of a favorable prognostic.

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Section: Discussionsupporting

confidence: 75%

Sinonasal Osteosarcoma in a Horse

Leite

Fabris

Silva

et al. 2019

Acta Scientiae. Vet.

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“…Luk et al, [51] had reported the characterization of osteosarcoma cell lines Saos-2 and U-2 OS successfully. In the canine osteosarcoma, the sarcosphers also showed strong calcification after three days in culture, as demonstrated by alizarin red staining [50].…”

Section: Discussionmentioning

confidence: 91%

“…We revealed that sarcospheres produced calcium in vitro. Sarcosphere showed the bright orange-red color that indicated the occurrence of alizarin red complex bonded with calcium [41,50]. Luk et al, [51] had reported the characterization of osteosarcoma cell lines Saos-2 and U-2 OS successfully.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Culture and Characterization of Cancer Stem Cells from Primary Osteosarcoma

Kamal¹,

Pranatha²,

Sugito³

et al. 2018

TOSCJ

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Background:Osteosarcoma cancer stem cells (CSCs) are defined as a subpopulation of osteosarcoma cells, which have the ability of self-renewal, proliferation and differentiation. This study aimed to identify CSCs from human osteosarcomain vitro.Methods:Osteosarcoma CSCs were isolated and cultured with sphere-forming assay technique on an ultra-low well attachment surface plate. After sarcosphere colonies were formed, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the expression of genes of embryonic stem cells such asNANOG, Oct3/4, STAT3 and gene of MSC CD133. Immunofluorescence analysis (IFA) of alkaline phosphatase (ALP), osteocalcin, and CD 133 was also performed to see the expression of osteosarcoma CSC surface protein with immuno-enzymatic staining principle. We also performed alizarin red staining to evaluate calcification in osteosarcoma CSCs.Results:The culture sphere-of the osteosarcoma cells showed three dimension round shaped colonies (sarcospheres) in slightly hypoxicand serum free condition which was not attached to the substrate with tight density. RT-PCR demonstrated that sarcospheres expressed genes which encodeNANOG, Oct3/4 STAT 3, but not for CD 133. IFA showed positive protein expression of ALP, osteocalcin and CD 133 which was moderate, strong, and weak positive respectively. Sarcospheres also had a positive reaction toward alizarin red staining.Conclusion:Osteosarcoma CSCs could be isolated from human osteosarcoma by sphere-forming assay technique and characterized by the expression of genes of embryonic stem cells,such asNANOG, Oct3/4, STAT3 and IFA of ALP, osteocalcin, and CD 133.

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“…Established cell lines have long been used to study tumor biology and response to targeted therapies. More recently, single gene evaluation and WES have been used to chart the mutational landscape of canine cancer cell lines, providing a crucial resource for prospective studies [1,[53][54][55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70]. The WGS data reported here identified many simple somatic mutations previously…”

Section: Discussionmentioning

confidence: 99%

The genomic landscape of canine osteosarcoma cell lines reveals conserved structural complexity and pathway alterations

et al. 2022

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The characterization of immortalized canine osteosarcoma (OS) cell lines used for research has historically been based on phenotypic features such as cellular morphology and expression of bone specific markers. With the increasing use of these cell lines to investigate novel therapeutic approaches prior to in vivo translation, a much more detailed understanding regarding the genomic landscape of these lines is required to ensure accurate interpretation of findings. Here we report the first whole genome characterization of eight canine OS cell lines, including single nucleotide variants, copy number variants and other structural variants. Many alterations previously characterized in primary canine OS tissue were observed in these cell lines, including TP53 mutations, MYC copy number gains, loss of CDKN2A, PTEN, DLG2, MAGI2, and RB1 and structural variants involving SETD2, DLG2 and DMD. These data provide a new framework for understanding how best to incorporate in vitro findings generated using these cell lines into the design of future clinical studies involving dogs with spontaneous OS.

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Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines

Cited by 9 publications

References 52 publications

Sinonasal Osteosarcoma in a Horse

Sinonasal Osteosarcoma in a Horse

Isolation, Culture and Characterization of Cancer Stem Cells from Primary Osteosarcoma

The genomic landscape of canine osteosarcoma cell lines reveals conserved structural complexity and pathway alterations

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