Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure

Abstract

Pericardial fluid, as a biochemical indicator of heart status, directly indicates pathological alteration to the heart. The accumulation of pericardial fluid can be attributed to an underlying systemic or local inflammatory process. However, the pericardial fluid expression of cellular surface markers, as well as several cytokines in chronic heart failure (CHF), remain unclear. In order to evaluate these issues further the pericardial fluid expression of several cytokines and the surface expression of activity markers between CHF patients and non-heart failure (NHF) patients were analyzed. The pericardial fluid expression of cytokines was measured by immunofluorescence and biomarker of plasma N-terminal propeptide of B-type natriuretic peptide (NT-proBNP), while pericardial fluid levels of soluble glycoprotein 130 (sgp130) were analyzed by ELISA in 50 CHF and 24 NHF patients. In addition, the surface expression of activation markers for T-cells was measured by immunohistochemistry. Patients with CHF demonstrated increased levels of plasma NT-proBNP and pericardial fluid sgp130. Surface expression of cellular activation markers CD25 and Foxp3 in the pericardial fluid was increased in patients with CHF. Moreover, the pro- and anti-inflammatory cytokines interferon (IFN)-γ, interleukin (IL)-6 and IL-10 in patients with CHF also demonstrated an increased expression within its pericardial fluid. In addition, there was infiltration of inflammatory cells and enhanced expression of inflammatory cytokines in the pericardial fluid of patients with CHF, which may reflect T cell activation, suggesting that systemic inflammation is important in the progression of CHF. This evidence could indicate a possible novel target for future therapeutics and prevention of CHF.

Isolation and Multiple Differentiation of Rat Pericardial Fluid Cells

“…Pericardial fluid could also be used as a signal to evaluate cardiac pathological changes ( Xiang et al, 2013 ; Foglio et al, 2015 ). A large amount of T cell infiltration and increased cytokines could be observed in the pericardial fluid of patients with chronic heart failure, leading to changes in the myocardial structural matrix and cause myocardial cell apoptosis that results in inflammation, which reflects T cell activation ( Iskandar et al, 2017 ). The tissue origins of PFCs remain unclear.…”

YQWY Decoction Improves Myocardial Remodeling via Activating the IL‐10/Stat3 Signaling Pathway

“…Enhanced proinflammatory cytokines secreted from these immune cells may contribute to the pathogenesis of myocardium dysfunction and the syndrome of HF [ 26 , 27 ]. Multiple clinical reports have revealed that patients with HF have elevated levels of inflammatory cytokines, such as TNF- α , IL-1 β , MCP-1, and IL-8 in circulation and myocardial tissues [ 28 , 29 ]. Increased circulating level of TNF- α appears to be associated with the deterioration of left ventricular function [ 30 ].…”

A native extracellular matrix material for tissue engineering applications: Characterization of pericardial fluid

“…Because of the content of PF changes in pathological situations, PF content can be a useful tool to detect pathological changes in the heart. 13,14 The porcine pericardium is commonly used in various applications, because of its high biocompatibility. It is FDA-approved for use in including stent covers, prosthetic valve materials, and the repair of soft tissue defects.…”

“…PF contents and amounts are used for diagnosing heart diseases. Because of the content of PF changes in pathological situations, PF content can be a useful tool to detect pathological changes in the heart 13,14 …”