Expression of p27 is associated with Bax expression and spontaneous apoptosis in oral and oropharyngeal carcinoma

Fujieda, Shigeharu; Inuzuka, Manabu; Tanaka, Nobuyuki; Sunaga, Hiroshi; Fan, Guo-Kang; Ito, Toshihisa; Sugimoto, Chizuru; Tsuzuki, Hideaki; Saitô, Hitoshi

doi:10.1002/(sici)1097-0215(19990621)84:3<315::aid-ijc20>3.0.co;2-u

Cited by 73 publications

(39 citation statements)

References 18 publications

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“…Overexpression of p27 by stable transfection triggers apoptosis in several human cancer cell lines 15 . In addition, we have found that p27 expression was correlated with spontaneous apoptosis in oral and oropharyngeal SCC 16 . Moreover, down‐regulation of p27 using antisense p27 cDNA led to a decrease in intercellular adhesion between cancer cells, suggesting that loss of p27 facilitates the metastasis of cancer cells by allowing the easy release of cancer cells from the original cancer tissue 17 .…”

Section: Discussionmentioning

confidence: 94%

Expression of protein p27 is associated with progression and prognosis in laryngeal cancer

et al. 1999

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Section: Discussionmentioning

confidence: 94%

Expression of protein p27 is associated with progression and prognosis in laryngeal cancer

et al. 1999

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“…In sharp contrast, a similar overexpression of p21/Cip1 results in G1‐S arrest, but minimum cytotoxicity was observed 33. Another study demonstrated that p27/Kip1 expression was associated with spontaneous apoptosis and Bax protein expression in tumor sections from oral and orthopharyngeal carcinoma in vivo and in vitro 34, 35. All these results imply that p27/Kip1 protein might play an important role in TB‐induced apoptosis, but not in cell growth arrest.…”

Section: Discussionmentioning

confidence: 96%

In vitro and in vivo studies of the anticancer action of terbinafine in human cancer cell lines: G0/G1 p53‐associated cell cycle arrest

Lee

Chen

Tseng

et al. 2003

Intl Journal of Cancer

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The current options for treating human cancer are limited to excision surgery, general chemotherapy, radiation therapy and, in a minority of breast cancers that rely on estrogen for their growth, antiestrogen therapy. Although there has been considerable improvement in the treatment of cancer, the overall prognosis remains not good. Therefore, investigators continue to search for new therapeutic strategies. One approach, as pursued in our study, seeks to identify medicinal agents capable of retarding the cell cycle and/or activating the cellular apoptotic response in the cancerous cells.Recently, we have shown that a number of antifungal agents exert antiproliferative and/or apoptotic activities in various malignant cells in vitro and in vivo. For instance, our previous studies showed that ketoconazole (Nizoral) induced cell cycle arrest at the G0/G1 phase of the cell cycle and the occurrence of apoptosis in hepatoma and colon cancer cells, 1,2 whereas griseofulvin (Grifulvin) induced apoptosis and cell cycle arrest at the G2/M phase through abnormal microtubule polymerization. 3 We also showed that combined treatment of griseofulvin and nocodazole (ND) significantly enhanced the therapeutic efficacy in the treatment of cancerous cells in athymic mice bearing COLO 205 tumor xenografts. 3 In the present study, we examined the antitumoral activity of terbinafine (TB) (Lamisil).TB is a newly synthesized oral antimycotic drug in the allylamines class: a fungicidal agent that inhibits ergosterol synthesis at the stage of squalene epoxidation. 4 It shows a good safety profile and relatively few drug interactions. 5 The cream form and oral tablet of TB have been approved for clinical uses in the United States. 6 The oral formulation has been on the market in various countries for more than 8 years, and as of 1997, more than 7.5 million individuals had been treated with this drug. 7 Here, we showed that TB inhibited the proliferation of tumor cells in vitro and in vivo. The experimental findings reported below highlight the molecular mechanisms of TB-induced antitumoral activity. MATERIAL AND METHODS Cell lines and cell cultureThe HT 29 (p53 mutant) 8 and COLO 205 (p53 wild) 9 cell lines were isolated from human colon adenocarcinoma (American Type

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“…Our previous study has shown, that differentiation of cells in tissue culture is associated with an increase in p27 expression [18]. Although a regular p27 expression protects normal cells from apoptosis, overexpression of p27 induces apoptosis of cells, through elevation of proapoptotic proteins, such as Bax [19,20]. These observations indicate that changes in p27 expression can determine the fate of muscle cells.…”

Section: Introductionmentioning

confidence: 99%

Abnormal expression of p27kip1 protein in levator ani muscle of aging women with pelvic floor disorders – a relationship to the cellular differentiation and degeneration

et al. 2001

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Background: Pelvic floor disorders affect almost 50% of aging women. An important role in the pelvic floor support belongs to the levator ani muscle. The p27/kip1 (p27) protein, multifunctional cyclin-dependent kinase inhibitor, shows changing expression in differentiating skeletal muscle cells during development, and relatively high levels of p27 RNA were detected in the normal human skeletal muscles.

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Expression of p27 is associated with Bax expression and spontaneous apoptosis in oral and oropharyngeal carcinoma

Cited by 73 publications

References 18 publications

Expression of protein p27 is associated with progression and prognosis in laryngeal cancer

Expression of protein p27 is associated with progression and prognosis in laryngeal cancer

In vitro and in vivo studies of the anticancer action of terbinafine in human cancer cell lines: G0/G1 p53‐associated cell cycle arrest

Abnormal expression of p27kip1 protein in levator ani muscle of aging women with pelvic floor disorders – a relationship to the cellular differentiation and degeneration

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