Expression of miR-302 in human embryo derived from in-vitro matured oocyte

Abstract

Background: The expression of miR-302 over the period of early embryogenesis could possibly regulate the maternal transcript clearance. Zygotic transcription activation is mostly related to maternal messages degradation.
 Objective: In this study, the effects of in-vitro maturation technique (IVM) on the expression of miR-302 in human embryo produced from immature and mature human oocytes (matured in vitro and in vivo, before sperm exposure) obtained from females under gonadotrophin therapy were evaluated for assisted reproduction.
 Materials and Methods: Immature oocytes were cultured in vitro. The injection of oocytes-producing polar bodies was given using fresh sperm. Then, the embryo quality score was assessed in the IVM group compared with the control group. In both the groups, embryos with normal morphology were included in the molecular study. Only one blastomere was removed from three-day embryos and then the embryos were frozen. The expression of miR-302 in embryos was measured through quantitative realtime polymerase chain reaction.
 Results: Our data showed a significant reduction of miR-302 expression in the IVM group vs. the control group (p = 0.02). The embryo quality score showed a significant difference between the two groups (p = 0.01).
 Conclusion: The present study demonstrated that the IVM process had a negative effect on the expression level of miR-302 in human pre-implantation embryos. Considering the major role of expression miR-302, a reduced potential in miR-302 expression could be related to a decrease in the early embryonic development.

Detection of Novel Potential Regulators of Stem Cell Differentiation and Cardiogenesis through Combined Genome-Wide Profiling of Protein-Coding Transcripts and microRNAs

“…Notably, several of the identified miRNAs have already been associated with development processes or used as biomarkers for the identification of different pathologies. Previous studies showed that the miR-302 family targets several genes that regulate cell cycle [ 54 ], and a decreased expression of members of this miRNA family affects early embryonic development [ 54 ]. In the context of heart development, miRNAs with peaks at days 8, 12 and 16 seem especially relevant.…”

Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application

“…1 ) and in molecular modulation ( Kashir et al , 2018 ). Other components at the transcriptional and translational level certainly play a role ( Dorfeshan et al , 2019 ; Rong et al , 2019 ; Sha et al , 2019 ; Zhang et al , 2019 ; Kang et al , 2020 ; Sacha et al , 2020 ; Sha et al , 2020 ; Zhang et al , 2020 ), but we focus herein on the wave-like Ca 2+ diffusion that is integral to oocyte activation.…”