Role of miRNAs in preimplantation embryo development and their potential as embryo selection biomarkers

Esmaeilivand, Masoumeh; Abedelahi, Ali; Hamdi, Kobra; Farzadi, Laya; Goharitaban, Sepide; Fattahi, Amir; Niknafs, Behrooz

doi:10.1071/rd21274

Cited by 5 publications

(2 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The search for non-invasive biomarkers came along with the evolution of embryo culture systems from undefined systems to complete chemicallydefined systems. Consequently, most of the already identified non-invasive biomarkers were detected in the spent media of embryos cultured under chemically-defined conditions (Hawke et al, 2021a;Navarro-Sánchez et al, 2022;Li et al, 2022;Esmaeilivand et al, 2022). Some research outputs on embryo biology demonstrate that the embryo is capable of modifying the maternal reproductive tract as early as at the blastocyst stage or even before.…”

Section: How Basic Science Could Helpmentioning

confidence: 99%

The embryo non-invasive pre-implantation diagnosis era: how far are we?

del Collado,

Andrade,

Gonçalves

et al. 2023

Anim. Reprod.

View full text Add to dashboard Cite

Advancements in assisted reproduction (AR) methodologies have allowed significant improvements in live birth rates of women who otherwise would not be able to conceive. One of the tools that allowed this improvement is the possibility of embryo selection based on genetic status, performed via preimplantation genetic testing (PGT). Even though the widespread use of PGT from TE biopsy helped to decrease the interval from the beginning of the AR intervention to pregnancy, especially in older patients, in AR, there are still many concerns about the application of this invasive methodology in all cycles. Therefore, recently, researchers started to study the use of cell free DNA (cfDNA) released by the blastocyst in its culture medium to perform PGT, in a method called non-invasive PGT (niPGT). The development of a niPGT would bring the diagnostics power of conventional PGT, but with the advantage of being potentially less harmful to the embryo. Its implementation in clinical practice, however, is under heavy discussion since there are many unknowns about the technique, such as the origin of the cfDNA or if this genetic material is a true representative of the actual ploidy status of the embryo. Available data indicates that there is high correspondence between results observed in TE biopsies and the ones observed from cfDNA, but these results are still contradictory and highly debatable. In the present review, the advantages and disadvantages of niPGT are presented and discussed in relation to tradition TE biopsy-based PGT. Furthermore, there are also presented some other possible non-invasive tools that could be applied in the selection of the best embryo, such as quantification of other molecules as quality biomarkers, or the use artificial intelligence (AI) to identify the best embryos based on morphological and/or morphokitetic parameters.

show abstract

Section: How Basic Science Could Helpmentioning

confidence: 99%

The embryo non-invasive pre-implantation diagnosis era: how far are we?

del Collado,

Andrade,

Gonçalves

et al. 2023

Anim. Reprod.

View full text Add to dashboard Cite

show abstract

“…8 Other candidates in implantation failure are low and high-expression endometrial miRNA. 5,9 miR-494 (antiimplantation) and miR-30d (pro-implantation) have different expressions in the endometrium of RIF patients compared to fertile women. 10 The use of calcitriol and letrozole in the treatment of infertility is common.…”

Section: Introductionmentioning

confidence: 99%

Study of LIF, NR3C1, BCL6, miR-494, and miR-30d in Endometrial Epithelial Cells of Repeated Implantation Failure and Healthy Women Treated with Calcitriol and Letrozole in a Fibrin Scaffold

Goharitaban,

Hamdi,

Khazaei

et al. 2022

ImmunoAnalysis

View full text Add to dashboard Cite

Background: Determining the exact time of window of implantation (WOI) in individuals with repeated implantation failure (RIF) is necessary for successful implantation. The present study aimed to compare the effects of letrozole and/or calcitriol on the expression of receptivity markers in endometrial epithelial cells (EECs) of RIF patients and healthy fertile women. Methods: Endometrial tissue was obtained from RIF patients (n=5) and healthy women (n=5) in the proliferation phase. EECs were isolated and cultured in a fibrin scaffold in 10 groups. The cells were treated with letrozole and/or calcitriol in three types of treatment. To check the proliferation and migration of cells in the scaffold, immunofluorescent and H&E staining was used, and gene expression was checked using real-time polymerase chain reaction (PCR). Results: This study showed that the expression levels of pro-implantation genes (NR3C1, LIF, and miR-30d) were higher in healthy women than in RIF patients, and the expression levels of anti-implantation genes (BCL6 and miR-494) were lower in healthy women than in RIF patients. Calcitriol and letrozole increased the expression level of pro-implantation genes (NR3C1, LIF, and miR-30d) in RIF patients. Furthermore, they reduced the expression level of anti-implantation genes (BCL6 and miR-494) in RIF patients. Conclusion: Our findings suggest that treatment with letrozole is more effective than co-treatment with letrozole and calcitriol or alone treatment with calcitriol on receptivity markers in RIF patients.

show abstract

Association of Trophectoderm mRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Esmaeilivand,

Ghasemzadeh,

Niknafs

et al. 2023

Reprod. Sci.

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) and mRNAs can serve as indicators of the chromosomal state of an embryo, with different pro les observed in euploid and aneuploid blastocysts. Examining the levels of miRNAs associated with aneuploidy and euploidy, as well as mRNAs related to implantation, can aid in predicting blastocyst chromosomal normality and improving assisted reproductive technology (ART) outcomes.This study analyzed chromosomal abnromality of 25 blastocysts using uorescence in situ hybridization (FISH) and also the expression of genes ERBB4, SELL, ITGB3, and ITGAV, as well as miRNAs, miR-339, miR-27b, miR-661, miR-30c, miR-191, miR-345, miR-142, miR-141, miR-20a and, miR-372. We found that 17 out of 25 embryos were aneuploid. Moreover, results revealed lower expression levels of miR-30c and

show abstract

Role of miRNAs in preimplantation embryo development and their potential as embryo selection biomarkers

Cited by 5 publications

References 67 publications

The embryo non-invasive pre-implantation diagnosis era: how far are we?

The embryo non-invasive pre-implantation diagnosis era: how far are we?

Study of LIF, NR3C1, BCL6, miR-494, and miR-30d in Endometrial Epithelial Cells of Repeated Implantation Failure and Healthy Women Treated with Calcitriol and Letrozole in a Fibrin Scaffold

Association of Trophectoderm mRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Contact Info

Product

Resources

About