Expression of markers for both neuronal and glial cells in human amniotic epithelial cells

Sakuragawa, Norio; Thangavel, Ramasamy; Mizuguchi, Masashi; Hirasawa, Motoyuki; Kamo, Isao

doi:10.1016/0304-3940(96)12599-4

Cited by 136 publications

(71 citation statements)

References 11 publications

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“…Our characterization is in accordance with previously published data, although most reports refer to human amniotic epithelial cells (HAEC), known to be part of the cellular content of amniotic fluid. In this regard, Sakuragawa et al [7] and Ishii et al [8] reported that this epithelial cell subpopulation derived from placenta may give rise to neurons and glial cells. In our hands, after the exposition to neural cell specific media, AFCs do not alter their morphology and no major modifications of neural cell markers were detected by RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

“…E-type cells have been thought to derive from fetal skin and urine, AF-type cells from fetal membranes and trophoblast and F-type cells from fibrous connective tissue and dermal fibroblasts [4][5][6]. Amniotic epithelial cells obtained from cesarean sections have been shown to express markers for neuronal, glial and progenitor cells and to differentiate into neuron-like cells in the ischemic brain of adult rats [7][8][9]. Moreover, there is evidence that amniotic fluid contains fetal mesenchymal stem cells with a multilineage differentiation potential [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

et al. 2006

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

et al. 2006

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“…We also reported the expression of Oct-4 in cells derived from the human amnion (Wei et al, 2003). In fact, hAE cells demonstrated the phenotypes and functions of pancreatic beta cells (Wei et al, 2003) and neural cells (Sakuragawa et al, 1996;Kakishita et al, 2000). Moreover, as the tissue is disposed of after parturition, it is easy to obtain and overcomes the ethical issues associated with use of fetal tissue.…”

Section: Introductionmentioning

confidence: 90%

Human Amniotic Epithelial Cells Possess Hepatocyte-like Characteristics and Functions

Takashima

Ise

Zhao

et al. 2004

Cell Struct. Funct.

133

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ABSTRACT. Hepatocyte transplantation is expected to become a novel method for treatment of liver disease. However, many questions remain regarding this approach, especially concerning donor cells. To evaluate whether human amniotic epithelial cells can be used as a cell source for hepatocyte transplantation, hepatic gene expression and functions of human amniotic epithelial cells were analyzed. Reverse transcription-polymerase chain reaction analysis demonstrated that human amniotic epithelial cells expressed albumin, α1-antitrypsin, and other hepatocyte-related genes. Cultivated human amniotic epithelial cells demonstrated albumin production, glycogen storage, and albumin secretion consistent with the hepatocyte gene expression profile. In organ culture, the amnion secreted 30-fold larger amounts of albumin than human amniotic epithelial cells in monolayer culture. Moreover, in organ culture the amnion also secreted α1-antitrypsin. Following transplantation into mice, the amnion survived and secreted albumin. These observations suggest that transplantation of human amniotic epithelial cells and/or amnion could be novel therapeutic strategy for treatment of hepatic diseases, including α1-antitrypsin deficiency.

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“…Pioneering research by Sakuragawa et al (1996) demonstrated not only that hAEC express markers of glial and neuronal progenitor cells, but also perform multiple neuronal functions such as synthesis and release of acetylcholine, catecholamines, neurotrophic factors, activin and noggin Koyano et al 2002;Sakuragawa et al 1997;Uchida et al 2000). As well as performing functions that are characteristic of neuronal cells, other studies report evidence that cells of the amniotic epithelium also provide support for growth of neuronal cell types in vitro.…”

Section: Human Amniotic Epithelial Cellsmentioning

confidence: 99%

Placenta-derived stem cells: new hope for cell therapy?

2008

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An urgent current need in regenerative medicine is that of identifying a plentiful, safe and ethically acceptable stem cell source for the development of therapeutic strategies to restore functionality in damaged or diseased organs and tissues. In this context, human term placenta represents a prime candidate, as it is available in nearly unlimited supply, is ethically problem-free and easily procured. Placental cells display differentiation capacity toward all three germ layers, while also displaying immunomodulatory effects, therefore supporting the possibility that they could be applied in an allogeneic transplantation setting. Although promising data have been reported to date, further study is required to fully characterize the differentiation potential of placenta-derived cells and to identify their possible clinical applications. Here, we provide a snapshot of current knowledge regarding the potential of cells from the amniotic membrane of human term placenta to address current shortcomings in the field of regenerative medicine.

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Expression of markers for both neuronal and glial cells in human amniotic epithelial cells

Cited by 136 publications

References 11 publications

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

Human Amniotic Epithelial Cells Possess Hepatocyte-like Characteristics and Functions

Placenta-derived stem cells: new hope for cell therapy?

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